대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)

  • 제23권2호
  • /
  • Pages.57-70
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  • 2010
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  • 1229-4292(pISSN)
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  • 2508-3619(eISSN)
대한한방부인과학회 (The Society of Korean Medicine Obstetrics and Gynecology)
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수지상세포에서 지대방(止帶方)의 T 세포 활성화에 미치는 영향

Study of Gidaebang on the T cells activation using dendritic cells

  • 김지양 (원광대학교 한의과대학 부인과학교실) ;
  • 정지혜 (원광대학교 한의과대학 부인과학교실) ;
  • 정현철 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실) ;
  • 조한백 (원광대학교 한의과대학 부인과학교실) ;
  • 김송백 (원광대학교 한의과대학 부인과학교실)
  • Kim, Ji-Ryang (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Jeong, Ji-Hye (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Cheong, Hyun-Cheol (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Choe, Chang-Min (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Cho, Han-Baek (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University) ;
  • Kim, Song-Baeg (Dept. of Oriental Obstetric and Gynecology, college of Oriental Medicine, Wonkwang University)
  • 투고 : 2010.04.28
  • 심사 : 2010.05.12
  • 발행 : 2010.05.31
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초록

Purpose: In this study, we investigated the effects of GDB(Gidaebang) on the immune response to establish the treatment mechanism of vaginitis. Methods: We examined the effects of GDB on the DCs(Dendritic cells) phenotypic and functional maturation. iDCs were cultured in the presence of GM-CSF and the generated iDCs were respectively stimulated by GDB or LPS as the control group for 24 hours. To evaluate the DCs phenotypic and functional maturation, we used flow cytometric analysis, RT-PCR and ELISA. Results: 1. GDB upregulated the expression of class II MHC and CD40 on DCs. 2. GDB upregulated the expression of CD80 and CD86 on DCs. 3. GDB induced cytokine IL-12 production and mRNA expression in DCs. Conclusion: These results suggest that GDB is able to improve the antigen-presenting capacity of DCs through the upregulation of their maturation, and might induce proliferation of T cells. In conclusion, this immunomodulatory properties of GDB may be useful in the treatment of vaginitis.

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