Mono sodium glutamate (MSG) 발효 GABA의 수면유도 효과

Sleep-Inductive Effect of GABA on the Fermentation of Mono Sodium Glutamate (MSG)

  • 김승섭 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 오성호 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 정명훈 (강원대학교 BT특성화학부대학 생물소재공학과) ;
  • 조석철 (경희대학교 피부생명공학센터) ;
  • 국무창 (바이오벤) ;
  • 이석호 (바이오벤) ;
  • 변유량 (바이오벤) ;
  • 이현용 (강원대학교 BT특성화학부대학 생물소재공학과)
  • Kim, Seung-Seop (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Oh, Sung-Ho (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Myoung-Hoon (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University) ;
  • Cho, Seok-Cheol (Skin Biotechnology Center, Kyung Hee University) ;
  • Kook, Moo-Chang (Biovan Co., Ltd.) ;
  • Lee, Seok-Ho (Biovan Co., Ltd.) ;
  • Pyun, Yu-Ryang (Biovan Co., Ltd.) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University)
  • 투고 : 2009.06.30
  • 심사 : 2009.10.31
  • 발행 : 2010.04.30

초록

미배아 발효로부터 얻은 GABA 시료를 이용하여 양성대조군으로 사용된 우유와 doxylamine succinate, 음성대조군으로 사용된 caffeine, 일반대조군으로 사용된 증류수와 함께 실험군인 GABA 시료를 농도별로 투여하여 실험을 수행하였다. 멜라토닌 함량을 측정한 결과, 대조군의 $2.607{\pm}0.410\;pg/mL$에 비해 GABA 60 mg/mL 투여군은 $3.243{\pm}0.154\;pg/mL$, GABA 120 mg/mL 투여군은 $3.425{\pm}0.182\;pg/mL$, 우유에 녹인 GABA 120 mg/mL 투여군은 $3.464{\pm}0.205\;pg/mL$으로 나타났다. 반면에 GABA 시료의 섭취 기간에 따른 멜라토닌 호르몬의 누적효과는 유의성이 없었다. 세로토닌 함량을 측정한 결과 대조군의 $4.73{\pm}0.67\;ng/mL$에 비해 GABA 60 mg/mL 투여군은 $4.71{\pm}1.22\;ng/mL$, GABA 120 mg/mL 투여군은 $5.37{\pm}0.96\;ng/mL$, 우유에 녹인 GABA 120 mg/mL 투여군은 $6.34{\pm}0.59\;pg/mL$으로 대조군의 평균값과 같거나 비교적 높은경향을 보였으며 농도별로 유의성이 있음을 확인하였다. 특히, GABA 120 mg/mL 투여시 멜라토닌과 세로토닌 모두 시판되는 수면보조제보다 높은 것으로 나타났다.

Relatively large amounts of GABA can be produced by the fermentation of rice bran. Therefore, this study was conducted to investigate the effects of GABA on the secretion of melatonin and serotonin for the development of a sleep inductive compound. The secretion levels of melatonin and serotonin from mice were found to be $3.425{\pm}0.182\;pg/mL$ and $5.37{\pm}0.963\;ng/mL$, respectively, in response to feeding 120 mg/mL of GABA while they were $2.607{\pm}0.41\;pg/mL$ in the control. The secretion of both melatonin and serotonin was increased up to the 13.51% and 34.99%, respectively, when compared to the negative control. However, the feeding of milk alone did not have a great effect on the melatonin and serotonin secretions. Conversely, feeding of milk with GABA enhanced the secretion of serotonin. The amounts of both melatonin and serotonin secreted increased with respect to the increase in GABA concentrations during feeding. Interestingly, the induction level of melatonin was relatively higher than that of serotonin in response to feeding 120 mg/mL of GABA. This is the first study to report that GABA has an ability to induce sleep related hormones in mice; therefore, it has the potential for use as a natural sleep aid.

키워드

참고문헌

  1. Kang TJ, Oh SH. Generation and utilization of GABA. Biowave 9: 1-18 (2007)
  2. Mody I, Dekoninck Y, Otis TS, Soltesz I. Bringing the cleft at GABA synapses in the brain. Trends. Neurosci. 17: 517-525 (1994) https://doi.org/10.1016/0166-2236(94)90155-4
  3. Abe Y, Umemura S, Sugimotto K, Hirawa N, Kato Y, Yokoyama T, Iwai J, Ishii M. Effect of green tea rich in γ-aminobutyric acid on blood pressure on Dahl salt-sensitive rats. Am. J. Hypertens. 8: 74-79 (1995) https://doi.org/10.1016/0895-7061(94)00141-W
  4. Namchuk M, Lindsay L, Turck CW, Kanaani J, Baekkeskov S. Phosphorylation of serine residues 3, 6, 10, and 13 distinguishes membrane anchored from soluble glutamic acid decarboxylase 65 and is restricted to glutamic acid decarboxylase $\alpha$6. J. Biol. Chem. 272: 1548-1557 (1997) https://doi.org/10.1074/jbc.272.3.1548
  5. Nomura M, Nakajima I, Fujita Y, Kobayashi M, Kimoto H, Suzuki I, Aso H. Lactococcus latis contains only one glutamate decarboxylase gene. Microbiol. 145: 1375-1380 (1999) https://doi.org/10.1099/13500872-145-6-1375
  6. Lee JY, Moon JS, Han BG, Yang HD, Kwon JB, Lee SI, Lee SS. The influence of acute cerebral infarction on the circadian rhythm of melatonin secretion. J. Korean Neurol. Assoc. 19: 359-363 (2001)
  7. Leventhal AG, Wang YC, Pu ML, Zhou YF, Ma Y. GABA and its agonists improved visual cortical function in senescent monkeys. Science 300: 812-815 (2003) https://doi.org/10.1126/science.1082874
  8. Oh SH, Oh CH. Brown rice extracts with enhanced levels of GABA stimulate immune cells. Food Sci. Biotechnol. 12: 248-252 (2003)
  9. Oh CH, Oh SH. Effect of geminated brown rice extract with enhanced levels of GABA on cancer cell proliferation and apoptosis. J. Med. Food. 7: 19-23 (2004) https://doi.org/10.1089/109662004322984653
  10. Kang SY, Lee KY, Park MJ, Kim YC, Maekelonis GJ, Oh TH, Kim YC. Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Neurobiol. Learn. Mem. 79 : 11-18 (2003) https://doi.org/10.1016/S1074-7427(02)00007-2
  11. Kook MC, Cho SC, Cheigh CI, Park H, Kim SS, Jeong MH, Pyun YR, Lee HY. Study of γ-aminobutyric acid (GABA) production by Lactobacillus sakei B2-16. Food. Eng. Prog. 13: 183-189 (2009)
  12. Yun JO, Kwon KI. Pharmacokinehcs of caffeine sensitive and non-senstive volunteers and in the obese rat and the lean rat. Korean Pharm. Sci. 37: 341-348 (1993)
  13. Zhang DJ, Elswick RK, Miller WG, Bailey JL. Effect of serumclot contact time on clinical chemistry laboratory results. Clin. Chem. 44: 1325-1333 (1998)
  14. Czeisler CA. Suppression of melatonin secretion in some blind patients by exposure to bright light. N. Engl. J. Med. 332: 6-11 (1995) https://doi.org/10.1056/NEJM199501053320102
  15. Hong YY, Moon IS, Kim DI, Lee TK. A study on melatonin, serotonin change, and behavior in the rats treated with Yiseontang, Gammakdaejotang, Sanjointang, and Sanjogammaktang. J. Orient. Gynecol. 12: 209-230 (1999)