Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Prediction and Annotation of ABC Transporter Genes from Magnaporthe oryzae Genome Sequence

벼도열병균 게놈서열로부터 ABC transporter 유전자군의 예측 및 특성 분석

  • Kim, Yong-Nam (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Jin-Soo (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Su-Young (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Jeong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Lee, Jong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Choi, Woo-Bong (Department of Biomaterial Control, Dong-Eui University)
  • 김용남 (동의대학교 바이오물질제어학과) ;
  • 김진수 (동의대학교 바이오물질제어학과) ;
  • 김수영 (동의대학교 바이오물질제어학과) ;
  • 김정환 (동의대학교 바이오물질제어학과) ;
  • 이종환 (동의대학교 바이오물질제어학과) ;
  • 최우봉 (동의대학교 바이오물질제어학과)
  • Received : 2009.12.02
  • Accepted : 2009.12.10
  • Published : 2010.02.28
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Abstract

Magnaporthe oryzae is destructive plant-pathogenic fungus and causes rice blast. The pathogen uses several mechanisms to circumvent the inhibitory actions of fungicides. ATP-binding cassette (ABC) transporters are known to provide protection against toxic compounds in the environment. PC facilitated bioinformatic analysis, particularly with respect to accessing and extracting database information and domain identification. We predicted ABC transporter genes from the M. oryzae genome sequence with computation and bioinformatics tools. A total of thirty three genes were predicted to encode ABC transporters. Three of thirty three putative genes corresponded to three known ABC transporter genes (ABC1, ABC2 and ABC3). Copy numbers of the ABC transporter genes were proven by Southern blot analysis, which revealed that twenty genes tested exist as a single copy. We amplified the DNA complementary to RNA corresponding to eleven of these by reverse transcriptase polymerase chain reaction.

벼의 생산에 있어 가장 큰 문제 요인 중 하나인 벼도열병의 발생 원인균인 벼도열병균은 다양한 기작에 의해 방제 약제에 대한 내성을 가지는 것으로 알려져 있다. 막 운반단백질인 ABC transporter의 경우 환경으로부터의 다양한 독성 물질들을 배출하는 것으로 알려져 있다. 이미 알려진 벼도열병균의 게놈 서열로부터 생물정보학적 분석을 통하여 ABC transporter 단백질의 도메인 특성을 보이는 33개의 유전자군 서열을 예측하였다. 이중 3개의 경우는 이미 알려진 유전자로 판명되었다. Southern Hybridization 분석에 적용한 20개의 유전자들이 모두 게놈상에 단일 copy로 존재함을 확인하였다. 새로 예측된 30개의 유전자중 11개는 RT-PCR을 통하여 전사단계에서의 유전자 발현이 확인되었다.

Keywords

References

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