Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibition of Cyclooxygenase-2 Activity and Prostaglandin E2 Production through Down-regulation of NF-κB Activity by the Extracts of Fermented Beans

발효 콩의 NF-κB 활성 억제를 통한 cyclooxgenase-2 활성과 prostaglandin E2 생성 억제

  • Lee, Hye-Hyeon (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Cheol (Blue-Bio Industry Regional Innovation Center, Dongeui University) ;
  • Kim, Min-Jeong (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Seo, Min-Jeong (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Choi, Sung-Hyun (Department of Elevator Maintenance, Korea Lift College) ;
  • Jeong, Yong-Kee (Department of Medical Bioscience, Graduate School and Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Choi, Yung-Hyun (Blue-Bio Industry Regional Innovation Center, Dongeui University)
  • 이혜현 (동아대학교 생명공학과 및 대학원 의생명과학과) ;
  • 박철 (동의대학교 블루바이오 소재개발센터) ;
  • 김민정 (동아대학교 생명공학과 및 대학원 의생명과학과) ;
  • 서민정 (동아대학교 생명공학과 및 대학원 의생명과학과) ;
  • 최성현 (한국승강기대학 승강기보수과) ;
  • 정영기 (동아대학교 생명공학과 및 대학원 의생명과학과) ;
  • 최영현 (동의대학교 블루바이오 소재개발센터)
  • Received : 2009.12.14
  • Accepted : 2010.01.07
  • Published : 2010.03.30
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Abstract

Cyclooxygenase (COX)-2 is generally known as an inducible enzyme, and it produces arachidonic acid to prostaglandin $E_2$ ($PGE_2$), which has been demonstrated to play a critical role in inflammation. In the present study, we investigated the effects of the extracts of fermented beans including soybean (FS), black agabean (FBA) and yellow agabean (FYA), on the expression of COXs and production of $PGE_2$ in U937 human promonocytic cells. Treatment of phorbol 12-myristate 13-acetate (PMA) significantly induced pro-inflammatory mediators such as COX-2 expression and $PGE_2$ production, whereas the levels of COX-1 remained unchanged. However, pre-treatment with FS, FBA and FYA significantly decreased PMA-induced COX-2 protein as well as mRNA, which is associated with inhibition of $PGE_2$ production. Moreover, FS, FBA and FYA markedly prevented the increase of nuclear translocation of nuclear factor kappa B (NF-${\kappa}B$) p65 by PMA. Our data indicate that the extracts of fermented beans exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-${\kappa}B$ signaling pathway.

본 연구에서는 콩 발효 산물 추출물의 항염증 효능에 관한 기초 자료를 제시하기 위하여 PMA에 의해 유도되는 COX-2의 발현 및 $PGE_2$의 생성 증가에 미치는 이들 추출물의 영향을 조사하였다. 본 연구에서 조사된 3가지 콩 발효 산물은 PMA에 의한 COX-2의 발현 증가를 매우 유의적으로 차단하였으며, 이는 $PGE_2$의 생성 억제와 연관성이 있었다. 아울러 PMA에 의한 NF-${\kappa}B$ 활성 증가 또한 콩 발효 산물들에 의하여 유의적으로 억제되었다. 이러한 결과들은 콩 발효 산물에 의한 NF-${\kappa}B$ 활성의 억제가 COX-2의 발현을 저하시켰으며, 이로 인한 $PGE_2$의 생성이 억제된 것으로 추정되어진다. 이러한 콩 발효 산물의 항염증 효과는 대두 추출물보다 아가콩 추출물에서 더욱 효과가 높게 나타났으며, 이는 향후 아가콩 추출물은 염증성 질환 예방/치료를 위한 적용 가능성이 매우 우수함을 제시하여 주는 결과이다.

Keywords

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