Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Verification of ET and AI Derived Offspring Using on the Genetic Polymorphisms of Microsatellite and Coat Color Related Genes in Jeju Black Cattle

제주흑우 집단에서 모색 관련 유전자와 microsatellite marker의 다형현상을 이용한 수정란이식 및 인공수정 유래 후대우 검증

  • Han, Sang-Hyun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Ko, Jin-Cheul (Institute for Livestock Promotion, Jeju Special Self-governing Province) ;
  • Kim, Young-Hoon (Institute for Livestock Promotion, Jeju Special Self-governing Province) ;
  • Kim, Nam-Young (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Jae-Hwan (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Ko, Moon-Suck (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Jeong, Ha-Yeon (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, In-Cheol (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Yang, Young-Hoon (Department of Animal Biotechnology, Jeju National University) ;
  • Lee, Sung-Soo (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
  • 한상현 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 고진칠 (제주특별자치도 축산진흥원) ;
  • 김영훈 (제주특별자치도 축산진흥원) ;
  • 김남영 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 김재환 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 고문석 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 정하연 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 조인철 (농촌진홍청 국립축산과학원 동물유전체과) ;
  • 양영훈 (제주대학교 동물자원과학과) ;
  • 이성수 (농촌진흥청 국립축산과학원 난지축산시험장)
  • Received : 2009.12.03
  • Accepted : 2010.01.14
  • Published : 2010.03.30
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Abstract

To find offspring of Jeju Black cattle (JBC) produced by embryo transfer (ET) and artificial insemination (AI), a molecular genetic study was carried out in candidate cattle populations collected from cattle farms in Jeju Island, Korea. The genetic marker set was composed of 11 ISAG microsatellite (MS) markers, 11 SAES MS markers selected by our preliminary analysis for population diversity of JBC and two major coat color related genes: MC1R and ASIP. The results showed a combined non-exclusion probability for first parent (NE-P1) that was higher than that recommended by ISAG (above 0.9995), and a combined non-exclusion probability for sib identity of $5.3{\times}10^{-10}$. Parentage analysis showed that the cases identified the candidate's father only (77.0%), mother only (54.0%), and both parents (40.5%) in the candidate offspring population. The ET and AI calves were identified as 14.7% in the in vitro fertilized eggs provided and 32.4% in total population, respectively. However, the result from ISAG marker analysis showed 3 identical allele-combinations in 7 calves, and that from ISAG/SAES MS marker combination also showed 1 identical allele-combination in 2 calves. Data from MS and coat-color gene analyses provided information for complete identification of all animals tested. Because the present JBC population was mostly bred using small nuclear founders through bioengineering techniques such as AI and ET, the genetic diversity levels obtained from MS analysis in the JBC population were relatively lower than those of other cattle populations, including Hanwoo. The results suggested that the more efficient marker combinations, including coat color related genotypes, should be studied and used for constructing a system for identification and molecular breeding of JBC as well.

농가에 보급된 제주흑우 수정란이식 및 인공수정 생산축의 확인을 위하여 분자유전학적 실험기법을 이용한 개체 추척을 수행하였다. 유전자 marker 체계는 ISAG 권장 MS marker 11종, 예비시험 후 선발된 SAES marker 11종, 흑모색 관련 MC1R과 ASIP 유전자들을 조합하여 분석하였다. 분석결과 부모 정보가 없는 상태에서의 부권 부정율이 국제권장기준보다 높은 수준을 보였으며, 형매간 동일개체출현률은 $5.3{\times}10^{-10}$으로 조사되었다. 친자검정 결과 후보축에 대한 후보 부, 모, 부모 모두가 확인되는 경우는 각각 77.0, 54.0, 40.5%였다. 부-모-자간 trio-mismatch가 전혀 없는 수정란이식 개체는 공급 수정란 대비 14.7%로 확인되었고, 전체 후보축군 중 32.4%는 후보 부와의 mismatch가 없는 인공수정에 의해 생산된 개체들로 판정하였다. ISAG marker들만을 분석한 결과에서는 7두가 동일한 3가지 유전자형 조합을 나타내었으나, ISAG/SAES marker들을 조합했을 때에는 2두에서만 동일 유전자형 조합을 나타내었다. MS와 모색유전자 분석자료를 모두 조합했을 때는 조사된 모든 개체들이 서로 구분되었다. 현재의 제주흑우집단이 소수 핵군에서 인공수정과 수정란이식 등 생명공학 기법으로 육성된 집단이기에 제주흑우집단의 유전적 다양성은 낮게 나타났다. 본 연구는 유전자 개체식별과 혈통관리 체계의 구축을 위해서는 적어도 20개 이상의 MS marker와 모색관련 유전자형 자료가 필수적으로 활용되어야함을 제안하고 있으며, 연구결과는 향후 제주흑우의 분자육종에 있어 유용한 자료가 될 것으로 시사하고 있다.

Keywords

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