생명과학회지 (Journal of Life Science)

  • 제20권3호
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  • Pages.374-380
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Biomass를 위해 제조한 청목노상 callus로부터 Helicobacter pylori 억제물질의 분리 및 동정

Purification and Identification of Inhibitory Compounds on Helicobacter pylori from Cheongmoknosang Callus for Biomass

  • 윤동혁 (경북대학교 식품공학과) ;
  • 차원섭 (경북대학교 식품공학과) ;
  • 이선호 (안동대학교 해양바이오산업연구소) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 김정환 (엔아이피 바이오텍) ;
  • 천성숙 (엔아이피 바이오텍) ;
  • 배종호 (대구미래대학 제과데코레이션과) ;
  • 조영제 (경북대학교 식품공학과)
  • 투고 : 2009.12.02
  • 심사 : 2009.12.20
  • 발행 : 2010.03.30
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초록

청목노상 잎 시료로부터 spot 배양 후 bioreactor에서 배양된 청목노상 뽕잎callus 추출물을 이용하여 H. pylori 균에 대한 항균활성을 측정한 결과, 높은 항균활성을 나타내는 것을 알 수 있었다. 배양된 청목노상 뽕잎 callus 추출물의 H. pylori 균에 대한 항균활성 물질을 정제하여 $^1H$-NMR, $^{13}C$-NMR, IR 및 FAB-MS spectrum 등을 활용하여 구조분석 한 결과 protocatechuic acid, chlorogenic acid, caffeic acid 및 rosemarinic acid로 동정하였으며, 단일물질 보다 혼합물질에 의한 synergy 효과에 의해 H. pylori 균에 대한 항균활성이 나타나는 것으로 판단되었다.

The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Cheomoknosang callus on Helicobacter pylori. Total phenolic compounds of 80% ethanol extracts from callus were 15.3 mg/g. The activity of H. pylori inhibition at 80% ethanol extracts from Cheongmoknosang callus was determined as 14 mm clear zone. Isolation of inhibitory compounds was carried out on Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in $H_2O$. The chemical structure of the inhibitory compound against Helicobacter pylori was confirmed as protocatechuic acid, chlorogenic acid, caffeic acid and rosemarinic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.

키워드

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피인용 문헌

  1. Evaluation of In Vivo Safety of Inhibitory Compounds from Cheongmoknosang Mulberry Leaves against Helicobacter pylori vol.40, pp.10, 2011, https://doi.org/10.3746/jkfn.2011.40.10.1404
  2. Evaluation of Medicinal Activity on Isolated Inhibitory Compounds against Helicobacter pylori from Cheongmoknosang Mulberry Leaves vol.54, pp.4, 2011, https://doi.org/10.3839/jabc.2011.043
  3. Inhibitory Activity against Helicobacter pylori of Isolated Compounds from Pinus koraiensis Siebold et Zucc Leaves vol.59, pp.1, 2016, https://doi.org/10.3839/jabc.2016.005
  4. Production of Inhibitory Compounds against Helicobacter pylori by Culture Condition of Morus alba cv. Cheongmoknosang Callus vol.23, pp.3, 2013, https://doi.org/10.5352/JLS.2013.23.3.368