생명과학회지 (Journal of Life Science)

  • 제20권4호
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  • Pages.623-631
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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노루궁뎅이(Hericium erinaceus) 자실체 추출 조다당류의 생쥐 Sarcoma 180에 대한 면역증강 및 항암 효과

Immuno-stimulating and Antitumor Effects on Mouse Sarcoma 180 by Crude Polysaccharides Extracted from Fruiting Body of Hericium erinaceus

  • 투고 : 2010.02.26
  • 심사 : 2010.04.12
  • 발행 : 2010.04.30
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초록

노루궁뎅이는 담자균문, 민주름버섯목, 산호침버섯과에 속하는 식의약용버섯으로 예로부터 위궤양이나 위암의 치료에 효과가 있으며 생쥐의 Sarcoma 180과 Ehrlich Sarcoma에도 효과가 있다고 알려져 왔다. 노루궁뎅이의 자실체로부터 중성염용액, 열수 및 메탄올 등을 이용하여 조다당류를 추출하고 ICR mice에 주사하여 항암 및 면역증강 효과를 조사하였다. Sarcoma 180, HepG2, HT-29, NIH3T3 등의 세포에 대한 세포독성을 조사한 결과 $10{\sim}2,000\;{\mu}g/ml$의 조다당류 농도에서 각각의 암세포는 세포독성을 거의 나타내지 않았다. Sarcoma 180으로 접종된 ICR mouse에 자실체에서 추출한 각각의 조다당류를 투여한 실험군은 대조군에 비해 수명이 각각 29.1~54.1% 연장되었다. 노루궁뎅이 자실체를 중성염 용액으로 추출한 조다당류는 $50\;{\mu}g/ml$의 농도에서 비장세포의 수를 2.9배 증가시켰으며 또한 중성염 추출 조다당류를 $200\;{\mu}g/ml$의 농도로 투여한 실험군 생쥐의 B 임파구 alkaline phosphatase의 활성은 대조군에 비해 5.5배의 증가하였다. 중성염 용액 추출 조다당류를 50 mg/kg body weight의 농도로 투여한 실험군 생쥐의 총 복강 세포 수와 백혈구의 수는 대조군에 비하여 각각 4배와 2.3배로 증가하였다. 또한 면역 관련 장기인 간, 비장 및 흉선의 체중에 대한 무게 비율은 대조군에 비하여 유의성을 보이지 않았다. 따라서 노루궁뎅이의 자실체에서 추출한 조다당류는 생쥐의 면역을 증강시키고 생쥐의 Sarcoma 180에 대해 항암효과를 나타내었다.

Hericium erinaceus, an edible and medicinal mushroom belonging to the Basidiomycota family, has been used for curing gastric ulcers and stomach cancers in human beings and is also known to have good inhibitory effects on sarcoma 180 and Ehrlich carcinoma in mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to as Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from the fruiting body of the mushroom. In in vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cell lines such as Sarcoma 180, HepG2, HT-29 and NIH3T3 at concentrations of $10{\sim}2,000\;{\mu}g/ml$. Intraperitoneal injection with crude polysaccharides exhibited a life prolongation effect of 29.1~54.1% in mice previously inoculated with Sarcoma 180. Fr. Na increased the numbers of spleen cells by 2.9 fold at a concentration of $50\;{\mu}g/ml$ compared with the control. Fr. Na improved the immuno-potentiating activity of B lymphocytes by increasing alkaline phosphatase activity by 5.5 fold compared with the control at a concentration of $200\;{\mu}g/ml$. Fr. NaCl increased the numbers of peritoneal exudate cells and circulating leukocytes by 4 and 2.3 folds at a concentration of 50 mg/kg, respectively. Therefore, the crude polysaccharides extracted from the fruiting body of H. erinaceus could improve antitumor activities in mice.

키워드

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