Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of a Carduus spp. Showing Anti-Mycobacterial Activity by DNA Sequence Analysis of Its ITS1, 5.8S rRNA and ITS2

Mycobacteria에 대해 항균력을 나타내는 엉겅퀴의 분류를 위한 ITS1, 5.8S rRNA, ITS2의 염기서열 분석

  • 배영민 (창원대학교 미생물학과)
  • Received : 2010.03.09
  • Accepted : 2010.04.19
  • Published : 2010.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

It has been reported that extracts of globe thistle (Echinops spp.) and thistle (Circium spp., Carduus spp. and Onopordum spp.) have anti-bacterial and anti-fungal activities. Methanol extracts of Echinops setifer and Carduus spp. were used to test and see if the extracts of these plants could suppress growth of Mycobacterium smegmatis and Mycobacterium fortuitum. Although extract of Echinops setifer showed no anti-mycobacterial activities, extract of Carduus spp. showed inhibition zones when tested with filter discs. Genomic DNA was isolated from Carduus spp. and PCR was performed to clone a DNA fragment containing ITS1, 5.8S rRNA gene and ITS2. A 733-bp PCR product was obtained and its DNA sequence was reported to the GenBank (accession number GU188570). BLAST search of the obtained DNA sequence did not show a match with any DNA sequences in the Genbank. Carduus crispus and Carduus defloratus had the closest phylogenetic relationships to this plant.

세균 및 진균류의 증식을 억제하는 능력이 있는 것으로 보고된 누로와 대계의 추출물을 사용하여 Mycobacterium smegmatis 및 Mycobacterium fortuitum의 증식을 억제하는 능력이 있는지를 시험하였다. 그 결과, 누로의 추출물에서는 증식억제능을 발견할 수 없었으나, 대계의 추출물에서는 뚜렷한 증식억제능이 관찰되었다. 따라서 본 연구에 사용된 대계(엉겅퀴)에 대한 분류학적 또는 진화적 분석을 수행하기 위하여 genomic DNA를 추출한 후, ITS1, 5.8S rRNA 유전자 및 ITS2를 포함하는 부분을 PCR로 증폭시켰다. PCR 산물의 염기서열을 분석한 결과, 733-bp의 염기서열이 얻어졌고, 이것을 GenBank에 등록하였다(accession number GU188570). 이렇게 얻어진 염기서열을 사용하여 BLAST analysis를 수행한 결과, 염기서열이 일치하는 생물체는 아직까지 GenBank에 보고된 적이 없고, 가장 가까운 식물들로는 귀화식물로서 전국적으로 분포하는 Carduus crispus (지느러미엉겅퀴) 및 현재까지 국내에 자생하는 것으로 보고된 적이 없는 Carduus defloratus로서 각각 3개씩의 염기가 다른 것으로 나타났다.

Keywords

References

  1. Barbour, E. K., M. Al Sharif, V. K. Sagherian, A. N. Habre, R. S. Talhouk, and S. N. Talhouk. 2004. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J. Ethnopharmacol. 93, 1-7. https://doi.org/10.1016/j.jep.2004.02.027
  2. Bremmer, K. 1994. Asteraceae: cladistics and classification. Timber Press. Poland, OR.
  3. Dye, C., M. A. Espinal, C. J. Watt, C. Mbianga, and B. G. Williams. 2002. World-wide incidence of multidrug-resistant tuberculosis. J. Infect. Dis. 185, 1197-1202. https://doi.org/10.1086/339818
  4. Fokialakis, N., C. L. Cantrell, S. O. Duke, A. L. Skaltsounis, and D. E. Wedge. 2006. Antifungal activity of thiophenes from Echinops ritro. J. Agric. Food Chem. 54, 1651-1655. https://doi.org/10.1021/jf052702j
  5. Haffner, E. and F. H. Hellwig. 1999. Phylogeny of the tribe Cardueae (Compositae) with emphasis on the subtribe Carduinae: an analysis based on ITS sequence data. Willdenowia 29, 27-39. https://doi.org/10.3372/wi.29.2902
  6. Heo, J. 2005. Dong-Eui-Bo-Gam. pp. 2977, 3006. Yeogang Press. Seoul.
  7. Hymete, A., T. H. Iversen, J. Rohloff, and B. Erko. 2005. Screening of Echinops ellenbeckii and Echinops longisetus for biological activities and chemical constituents. Phytomedicine 12, 675-679. https://doi.org/10.1016/j.phymed.2004.01.013
  8. Kelch, D. G. and B. G. Baldwin. 2003. Phylogeny and ecological radiation of New World thistles (Cirsium, Cardueae - Compositae) based on ITS and ETS rDNA sequence data. Mol. Ecol. 12, 141-151. https://doi.org/10.1046/j.1365-294X.2003.01710.x
  9. Kress, W. J., K. J. Wurdack, E. A. Zimmer, L. A. Weigt, and D. H. Janzen. 2005. Use of DNA barcodes to identify flowering plants. Proc. Natl. Acad. Sci. USA 102, 8369-8374. https://doi.org/10.1073/pnas.0503123102
  10. Jeong, D. M., H. A. Jung, and J. S. Choi. 2008. Comparative antioxidant activity and HPLC profiles of some selected Korean thistles. Arch. Pharm. Res. 31, 28-33. https://doi.org/10.1007/s12272-008-1116-7
  11. Lee, T. B. 2003. Coloured flora of Korea. pp. 183, Hyangmun Publishing Co. Seoul.
  12. Lee, W. B., H. C. Kwon, O. R. Cho, K. C. Lee, S. U. Choi, N. I. Baek, and K. R. Lee. 2002. Phytochemical constituents of Cirsium setidens Nakai and their cytotoxicity against human cancer cell lines. Arch. Pharm. Res. 25, 628-635. https://doi.org/10.1007/BF02976934
  13. Lee, Y. M., S. H. Park, J. C. Yang, and H. J. Choi. 2008. Two new naturalized species from Korea, Carduus natans and Lepidium latifolium. Korean J. Pl. Taxon. 38, 187-196.
  14. Liu, S., X. Luo, D. Li, J. Zhang, D. Qiu, W. Liu, L. She and Z. Yang. 2006. Tumor inhibition and improved immunity in mice treated with flavone from Cirsium japonicum DC. Int. Immunopharmacol. 6, 1387-1393. https://doi.org/10.1016/j.intimp.2006.02.002
  15. Liu, S., J. Zhang, D. Li, W. Liu, X. Luo, R. Zhang, L. Li, and J. Zhao. 2007. Anticancer activity and quantitative analysis of flavone of Cirsium japonicum DC. Nat. Prod. Res. 21, 915-922. https://doi.org/10.1080/14786410701494686
  16. Loizzo, M. R., G. A. Statti, R. Tundis, F. Conforti, S. Ando, and F. Menichini. 2004. Antimicrobial activity and cytotoxicityb of Cirsium tenoreanum. Fitoterapia. 75, 577-580. https://doi.org/10.1016/j.fitote.2004.03.011
  17. Nazaruk, J. and J. Gudej. 2003. Flavonoid compounds from the flowers of Cirsium rivulare (Jacq.) All. Acta. Pol. Pharm. 60, 87-89.
  18. Nazaruk, J. and P. Jakoniuk. 2005. Flavonoid composition and antimicrobial activity of Cirsium rivulare (Jacq.) All. flowers. J. Ethnopharmacol. 102, 208-212. https://doi.org/10.1016/j.jep.2005.06.012
  19. Park, J. C., J. M. Hur, J. G. Park, S. C. Kim, J. R. Park, S. H. Choi, and J. W. Choi. 2004. Effects of methanol extract of Cirsium japonicum var. ussuriense and its principle, hispidulin-7-O-neohesperidoside on hepatic alcohol-metabolizing enzymes and lipid peroxidation in ethanol-treated rats. Phytother. Res. 18, 19-24. https://doi.org/10.1002/ptr.1299
  20. Park, J. H. 2004. Atlas of Korean Medicinal Plants. pp.1201-1202, Shin-Il Press. Seoul.
  21. Segovia-Juarez, J. L., S. Ganguli and D. Kirschner. 2004. Identifying control mechanisms of granuloma formation during M. tuberculosis infection using an agent-based model. J. Theor. Biol. 231, 357-376. https://doi.org/10.1016/j.jtbi.2004.06.031
  22. Song, M. J. and H. Kim. 2007. Taxonomic study on Cirsium Miller (Asteraceae) in Korea on based on external morphology. Korean J. Pl. Taxon. 37, 17-40.
  23. Wang, Y. J., J. Q. Liu, and G. Miehe. 2007. Phylogenetic origins of the Himalayan endemic Dolomiaea, Diplazoptilon and Xanthopappus (Asteraceae: Cardueae) based on three DNA regions. Ann. Bot. 99, 311-322.

Cited by

  1. Phylogenetic and Chemical Analyses of Cirsium pendulum and Cirsium setidens Inhabiting Korea vol.22, pp.8, 2012, https://doi.org/10.5352/JLS.2012.22.8.1120