Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Aged Black Garlic Extracts on the Tight Junction Permeability and Cell Invasion in Human Gastric Cancer Cells

흑마늘 추출물이 인체위암세포의 tight junction 투과성 조절과 세포 침윤성 억제에 미치는 영향

  • Shin, Dong-Yeok (Department of Biochemistry, College of Oriental Medicine, Dongeui University) ;
  • Yoon, Moo-Kyoung (Vegetable Research Division, National Institute of Horticulture & Herbal Science) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resource and Life Sciences, Pusan National University) ;
  • Gweon, Oh-Cheon (Department of Hotel Culinary Arts & Bakery, Gyeongnam Provincial Namhae College) ;
  • Kim, Jung-In (School of Food and Life Science, Inje University) ;
  • Choi, Tae-Hyun (Department of Ophthalmic Optics, Daegu Technical University) ;
  • Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
  • 신동역 (동의대학교 한의과대학 생화학교실) ;
  • 윤무경 (국립원예특작과학원 채소과) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 권오천 (경남도립남해대학 호텔조리제빵과) ;
  • 김정인 (인제대학교 식품생명과학부) ;
  • 최태현 (대구공업대학 안경광학과) ;
  • 최영현 (동의대학교 한의과대학 생화학교실)
  • Received : 2010.02.11
  • Accepted : 2010.02.17
  • Published : 2010.04.30
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Abstract

Garlic (Allium sativum) has been well-known as a folk remedy for a variety of ailments since ancient times, and it is well documented that enhanced garlic consumption leads to a decrease in incidences of cancer. Tight junctions (TJs) are critical structures for the maintenance of cellular polarity, acting as paracellular permeability barriers and playing an essential role in regulating the diffusion of fluid, electrolytes and macromolecules through the paracellular pathway. Matrix metalloproteinases (MMPs) have been implicated as possible mediators of invasiveness and metastasis in some cancers. In this study, we investigated the potential effects of water extract of aged black garlic (ABG) on the correlation between tightening of TJs and anti-invasive activity in human gastric carcinoma AGS cells. The inhibitory effects of ABG on cell motility and invasiveness were found to be associated with increased tightness of TJs, which was demonstrated by an increase in transepithelial electrical resistance. Additionally, the activities of MMP-2 and -9 in AGS cells were inhibited by treatment with ABG, and this was also correlated with a decrease in the expression of their mRNA and proteins. Furthermore, RT-PCR and immunoblotting results indicated that ABG repressed the levels of the claudin proteins, major components of TJs that play a key role in the control and selectivity of paracellular transport. In conclusion, these results suggest that ABG treatment may inhibit tumor metastasis and invasion, and therefore may act as a dietary source to decrease the risk of developing cancer.

본 연구에서는 흑마늘 열수 추출물(ABG)의 암세포 전이억제 효능을 검정하였다. AGS AGS 인체위암세포의 이동성과 침윤성을 억제하였으며, 이는 TER의 증가와 연관성이 있었다. 또한 ABG에 의한 AGS 위암세포의 침윤성 억제는 TIMPs 발현 증가에 의한 MMPs의 발현 및 활성 저하에 의한 것임을 알 수 있었다. 아울러 AGS 위암세포에서 과발현을 나타내는 TJ 단백질인 claudins의 발현 저하 등이 ABG의 항전이 효과에 연관되어 있음을 알 수 있었다. 이상의 결과는 흑마늘 열수 추출물이 단순한 암세포의 증식억제를 통한 암예방 및 항암활성 뿐만 아니라 암세포의 전이 또한 효과적으로 억제할 수 있음을 보여주며, 이와 연관된 보다 구체적인 분자세포생물학적 접근 및 in vivo 연구의 필요성이 요구됨을 의미한다.

Keywords

References

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