생명과학회지 (Journal of Life Science)

  • 제20권4호
  • /
  • Pages.487-495
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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해양미생물 Psychrobacter aquimaris LBH-10가 생산하는 산성 carboxymethylcellulase의 특성에 대한 연구

Characterization of Acidic Carboxymethylcellulase Produced by a Marine Microorganism, Psychrobacter aquimaris LBH-10

  • 김혜진 (동아대학교 대학원 의생명공학과) ;
  • 고와 (동아대학교 대학원 의생명공학과) ;
  • 이유정 (동아대학교 대학원 의생명공학과) ;
  • 정정한 (동아대학교 BK21 생물자원 실버바이오 산업화 인력양성 사업단) ;
  • 이진우 (동아대학교 BK21 생물자원 실버바이오 산업화 인력양성 사업단)
  • Kim, Hye-Jin (Department of Medical Bioscience, Graduate School of Donga-A University) ;
  • Gao, Wa (Department of Medical Bioscience, Graduate School of Donga-A University) ;
  • Lee, You-Jung (Department of Medical Bioscience, Graduate School of Donga-A University) ;
  • Chung, Chung-Han (BK21 Bio-Silver Project of Dong-A University) ;
  • Lee, Jin-Woo (BK21 Bio-Silver Project of Dong-A University)
  • 투고 : 2010.01.18
  • 심사 : 2010.03.08
  • 발행 : 2010.04.30
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초록

Carboxymethylcellulose (CMC)를 분해하는 미생물을 해수에서 분리하였으며, 16S rDNA의 염기서열을 분석하여 동정한 결과, Psychrobacter aquinaris로 학인 되어 P. aquinaris LBH-10로 명명하였다. 이 균주는 CMC, 셀로바이오스, 커드란, 여과지, p-nitrophenyl-$\beta$-D-glucopyranoside (pNPG), 풀루란 및 자일란을 분해하였으나, avicel 및 섬유소는 분해하지 못하였다. P. aquinaris LBH-10이 생산하는 carboxymethylcellulase (CMCase)의 최적 반응 온도는 $50^{\circ}C$이었으며, $20^{\circ}C$에서 $50^{\circ}C$의 온도 범위에서 24시간이 경과한 후에도 90% 이상의 활성을 유지하였다. 또한, 이 균주가 생산하는 CMCase의 최적 반응 pH는 3.5이었으며 pH 2.5에서 pH 7.0 사이의 산성 조건하에서 24시간이 경과한 후에도 70% 이상의 활성을 유지하였다. P. aquinaris LBH-10이 생산하는 CMCase의 최적 반응 pH는 지금까지 발견된 섬유소 분해효소 중에서 가장 낮은 pH로 판단된다. 제한된 농도의 $CoCl_2$, EDTA, 및 $PbCl_2$은 P. aquinaris LBH-10가 생산하는 CMCase의 활성을 증가시켰으나, $HgCl_2$, KCl, $MnCl_2$, $NiCl_2$, 및 $SrCl_2$는 이 균주가 생산하는 CMCase의 활성을 감소시켰다.

A microorganism hydrolyzing carboxymethylcellulose (CMC) was isolated from seawater, identified as Psychrobacter aquimaris by analysis of 16S rDNA sequences, and named P. aquimari LBH-10. This strain produced an acidic carboxymethylcellulase (CMCase), which hydrolyzed carboxymethylcellulose (CMC), cellobiose, curdlan, filter paper, p-nitrophenyl-$\beta$-D-glucopyranoside (pNPG), pullulan, and xylan, but there was no detectable activity on avicel and cellulose. The optimal temperature for CMCase produced by P. aquimari LBH-10 was $50^{\circ}C$ and more than 90% of its original activity was maintained at broad temperatures ranging from 20 to $50^{\circ}C$ after 24 hr. The optimal pH of the CMCase was 3.5, and more than 70% of its original activity was maintained under acidic conditions between pH 2.5 and 7.0 at $50^{\circ}C$ after 24 hr. The optimal pH of CMCase produced by P. aquimaris LBH-10 seems to be lower than those produced by any other bacterial and fungal strain. $CoCl_2$, EDTA, and $PbCl_2$ at a concentration of 0.1 M enhanced CMCase-produced P. aquimaris LBH-10, whereas $HgCl_2$, KCl, $MnCl_2$, $NiCl_2$, and $SrCl_2$ inhibited it.

키워드

과제정보

연구 과제 주관 기관 : Korea Science and Engineering Foundation (KOSEF)

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