생명과학회지 (Journal of Life Science)

  • 제20권4호
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  • Pages.467-473
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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NIH-미니돼지의 간과 심장에서 갱글리오시드의 서로 다른 발현 패턴

Differential Expression Patterns of Gangliosides in the Liver and Heart of NIH-miniature Pigs

  • 유재성 (원광대학교 자연과학대학 생명과학부) ;
  • 장규태 (한국생명공학연구원 국가영장류센터) ;
  • 김지수 (한국생명공학연구원 국가영장류센터) ;
  • 곽동훈 (원광대학교 자연과학대학 생명과학부) ;
  • 이영춘 (동아대학교 생명자원과학대학 생명공학과) ;
  • 오건봉 (농촌진흥청 국립축산과학원 축산생명환경부 동물바이오공학과) ;
  • 추영국 (원광대학교 자연과학대학 생명과학부)
  • Ryu, Jae-Sung (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Chang, Kyu-Tae (National Primate Research Center (NPRC), Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Kim, Ji-Su (National Primate Research Center (NPRC), Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Kwak, Dong-Hoon (Department of Biological Science, College of Natural Sciences, Wonkwang University) ;
  • Lee, Young-Choon (Department of Biotechnology, College of Natural Resources and Life Sciences, Dong-A University) ;
  • Oh, Keon-Bong (Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choo, Young-Kug (Department of Biological Science, College of Natural Sciences, Wonkwang University)
  • 투고 : 2009.12.02
  • 심사 : 2010.02.22
  • 발행 : 2010.04.30
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초록

갱글리오시드는 포유동물 세포막의 중요한 구성요소로서 세포와 세포 혹은 세포와 단백질간의 상호작용을 포함한 다양한 면역학적 역할을 수행하고 있다. 이 연구는 NIH-미니돼지의 간과 심장을 인간에게 이식할려고 할 때 예측되어지는 거부 반응과 관련된 구성성분들 중 시알산을 함유하고 있는 스핑고당지질인 갱글리오시드에 대해 조사하였다. 얇은막크로마토그래피와 면역조직화학적분석을 실시한 결과 NIH-미니돼지의 간은 갱글리오시드의 발현이 심장보다 높게 나타났다. 갱글리오시드 GD3, GD1a, GD1b, GT1b는 간과 심장의 두 기관에서 발견되었다. 그러나 GQ1b는 간에서만 발견되었고 심장에서는 검출되지 않았다. 이러한 결과는 갱글리오시드의 발현양상은 간과 심장에서 조직특이적이라는 것을 의미한다. 한편, GM3를 포함한 다른 갱글리오 시리즈인 갱글리오시드들은 NIH-미니돼지의 간과 심장에서 검출되어지지 않았다. 이와 같은 연구결과로부터 갱글리오시드는 미니돼지의 장기중 특히, 간과 심장의 이종장기이식과 관련된 면역거부반응에서 어떤 역할을 수행하고 있다고 여겨진다.

Gangliosides are a major component of the plasma membrane of mammalian cells, which are directly involved in a variety of immunological events, including cell-to cell or cell-to-protein interactions. In this study, we investigated whether gangliosides, sialic acid-containing glycosphingolipids, are related to rejection during the xenotransplantation of NIH-miniature pig livers and hearts to humans. Both high performance thin-layer chromatography and immunohistochemistry analyses revealed that the expression of gangliosides in the liver tissue of NIH-miniature pigs was higher than that in the heart. Gangliosides GD3, GD1a, GD1b, GT1b and GQ1b were observed in both the liver and heart, whereas GQ1b was detected only in the liver, indicating that the ganglioside expression profiles are tissue specific. Moreover, other ganglio-series gangliosides, including GM3, were not detected in the livers and hearts of NIH-miniature pigs. Taken together, these results suggest that gangliosides may play important roles in immune responses in clinical xenotransplants of pig livers and hearts.

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