The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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The Preliminary Study for Therapeutic Mechanism of Gami-Bojungikgitang and Gami-Jwagwieum for Pulmonary Fibrosis

가미보중익기탕과 가미좌귀음의 폐 섬유화 치료 기전에 대한 예비 연구

  • Lee, Hai-Ja (Department of Pediatrics, College of Oriental Medicine, WonKwang University) ;
  • Shin, Gwon-Sung (Department of Pediatrics, College of Oriental Medicine, WonKwang University) ;
  • Ahn, Jae-Sun (Department of Pediatrics, College of Oriental Medicine, WonKwang University)
  • 이해자 (원광대학교 한방소아과) ;
  • 신권성 (원광대학교 한방소아과) ;
  • 안재선 (원광대학교 한방소아과)
  • Received : 2010.04.03
  • Accepted : 2010.04.24
  • Published : 2010.04.30
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Abstract

Objectives In this study, we evaluated the therapeutic effects of Gami-Bojungikgitang and Gami-Jwagwieum for bleomycin-induced lung fibrosis in mice. Methods Extracted lyophilization, Gami-Bojungikgitang (96g) and Gami-Jwagwieum (118g) boiled, filtered, depressed, concentrated, and are obtained. They were divided into five groups: normal, group IA; Animal group treated with bleomycin observed on the 21th day, group IB; Animal group treated with bleomycin observed on the 42th day, group IIA; Animal group treated with bleomycin and Gami-Bojungikgitang. Gami-Jwagwieum observed on the 21th day, group IIB; Animal group treated with bleomycin and Gami-Bojungikgitang/Gami-Jwagwieum observed on the 42th day. Mice are used on the 42th day and as a result, bronchoalveolar lavages fluid is obtained. Counting total number of cells, different ratio of macrophage, lymphocyte, and neutrophil are established. Results In animal group treated with bleomycin and Gami-Bojungikgitang, total cell count decreased by 50% in 3 weeks compared to animal group with non-administrated Gami-Bojungikgitang. However, total cell count in 6 weeks increased compared to 3 weeks although total cell count still decreased compared to animal group with non-administrated Gami-Bojungikgitang. In the view of differential cell counts in bronchoalveolar lavages fluid in treatment group on 3 and 6 weeks, neutrophile was a few and lymphocyte decreased. In animal group treated with bleomycin and Gami-Jwagwieum, total cell count decreased by 50% in 3 and 6 weeks compared to animal group with non-administrated Gami-Jwagwieum. In the view of differential cell counts in bronchoalveolar lavages fluid in treatment group on 3 and 6 weeks, lymphocyte also decreased. Conclusions Gami-Bojungikgitang and especially Gami-Jwagwieum for bleomycin-induced lung fibrosis in mice were effective in total cell count and differential cell count.

Keywords

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