The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지

Effects of SPDJTK(SoPungDoJeokTangKami) and Concurrent Administration of AJ (Atopy cream, Jawoongo) Plus SPDJTK on Atopic Dermatitis-like Skin Lesions in NC/Nga Mouse Induced by BMAC

아토피양(樣) 피부염 NC/Nga 생쥐에서 소풍도적탕가미(消風導赤湯加味)와 아토피크림, 자운고(紫雲膏) 및 소풍도적탕가미(消風導赤湯加味)의 병용투여가 피부염에 미치는 영향

  • Han, Dal-Soo (Department of Pediatrics, College of Oriental Medicine, Dae Jeon University) ;
  • Han, Jae-Kyung (Department of Pediatrics, College of Oriental Medicine, Dae Jeon University) ;
  • Kim, Yun-Hee (Department of Pediatrics, College of Oriental Medicine, Dae Jeon University)
  • 한달수 (대전대학교 한의과대학 소아과학교실) ;
  • 한재경 (대전대학교 한의과대학 소아과학교실) ;
  • 김윤희 (대전대학교 한의과대학 소아과학교실)
  • Received : 2010.03.21
  • Accepted : 2010.04.24
  • Published : 2010.04.30
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Abstract

Objectives The purpose of this study is to investigate the effect of SPDJTK(SoPungDoJeokTangKami) and concurrent administration of AJ(Atopy cream, Jawoongo)+SPDJTK on atopic dermatitis-like skin lesions by using in NC/Nga atopic dermatitis mouse induced by BMAC-induced mice. Methods Clinical skin score, hematology and Serum total IgE and IgG1 of NC/Nga atopic dermatitis mice were evaluated. Moreover, the cytokine level, total cell number, Immunohistochemical staining and Histological features of axillary lymph node(ALN), draining lymph node(DLN), peripheral blood mononuclear cells(PBMCs) and dorsal skin tissue were used in NC/Nga mice. Results Orally administrated SPDJTK with concurrent administration of SPDJTK and AJ decreased the clinical skin score, total cell number of WBC, eosinophils in blood, serum total IgE & IgG1, IL-5, IL-13, IFN-$\gamma$. Also, total cell number of ALN and dorsal skin tissue, absolute cell number of CD4+, CD8+, CD3+CD69+, CD3+CCR3+, CCR3+, CD4+CXCR5+ in ALN, absolute cell number of CD3+CCR3+, CCR3+ in DLN, granulocytes in PBMCs, activation cell number of CD3+CD69+, CCR3+, total cell number of CD3+ T cell in dorsal skin tissue were significantly decreased. Furthermore, thickness of epidermis, infiltrated inflammatory immune cell and mast cell in dermis, amount of Eotaxin2 mRNA, CCR3 mRNA in dorsal skin tissue, gene expression of IL-5, IL-13 mRNA in ALN, CD4+ Th cell in dorsal skin tissue and CCR3+ eosinophils in ALN were all significantly decreased. However, total number of DLN, absolute number of CD3e+ T cell and CD19+ B cell, absolute number of CD4+, number of Th cell in DLN and gene expression of foxp3 mRNA were significantly increased significantly. Conclusions Concurrent administration of SPDJTK and AJ on atopic dermatitis in NC/Nga atopic dermatitis mouse was very effective treatment for atopic dermatitis.

Keywords

References

  1. 丁奎萬. Allergy와 韓方. 서울:第一路. 1990:101-108.
  2. 장호선. 아토피 피부염의 진단과 치료. 가정의학회지. 2002;23:831-838.
  3. Morren M, Przybilla B, Bamelis M, et al.. Atopic dermatitistriggering factors. J Am Acad Dermatol. 1994;467-473.
  4. 박민철, 김진만, 홍철희, 황충연. 아토피 피부염의동, 서의학적 문헌고찰. 대한안이비인후피부과학학회지. 2002;15(1):226-252.
  5. 馬紹堯. 現代中醫皮膚性病學. 上海:上海中醫藥大學出版社. 2001:153-60.
  6. 金起鳳, 周德瑛 主編. 中醫皮膚病學. 北京:中國醫藥科技出版社. 1999:193-8
  7. 馬紹堯, 趙尙華 主編. 現代中醫皮膚性病診療大全. 山西:山西科學技術出版社. 1999: 475-93.
  8. Halbert AR, Weston WL, Moreli JG. Atopic dermatitis : is it an allergic disease?. J Am Acad Dermatol. 1995;33:1008-1018. https://doi.org/10.1016/0190-9622(95)90295-3
  9. Leung DYM. Pathogenesis of atopic dermatitis. J Allergy Clin Immunol. 1999;104:99-108. https://doi.org/10.1016/S0091-6749(99)70150-8
  10. Mosmann TR, Coffman RI. Th1 and Th2 cells : Different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 1989;7:145-73. https://doi.org/10.1146/annurev.iy.07.040189.001045
  11. Tone Y, Furuuchi K, Kojima Y, Tykocinski ML, Greene MI, Tone M.. Smad3 and NFAT cooperate to induce foxp3 expression through its enhancer. 2008;9(2):194-202.
  12. Guin JD. Complications of topical hydrocortisone. J Am Acad Dermatol. 1981;4:417-22. https://doi.org/10.1016/S0190-9622(81)70040-9
  13. Graham-Brown R. Managinf adults with atopic dermatitis. Dermatol Clin. 1996;14:531-7. https://doi.org/10.1016/S0733-8635(05)70381-8
  14. Smith CH. New approaches to atopical therapy. Clin Exp Dermatol. 2000;25:250-4. https://doi.org/10.1046/j.1365-2230.2000.00628.x
  15. 허인희, 홍성민, 엄유식, 변학성, 심성용, 김경준. 외치요법을 위주로 한 아토피 피부염 환자 증례. 한방안이비인후과피부과학회지. 2006;9(3):201-208.
  16. 顧伯華. 實用中醫外科學. 上海:上海科學技術出版社. 1994:461.
  17. 황치환․윤채성․송승현․원영호․황충연. 葛根解肌湯加味方과 三黃洗劑加味方 병용이 NC/Nga생쥐의 아토피 피부염에 미치는 영향. 한방안이비인후피부과학회지. 2008;21(2):1-18.
  18. 민영규, 최정화, 김종한, 박수연. 消風散加減方과 外治方 병용이 NC-Nga 아토피 생쥐에 미치는 영향. 대한이비인후피부과학회지. 2005;18(1):116-134.
  19. 홍철희, 서은성, 원영호, 김영근, 황충연. 溫淸飮과 三黃洗劑加味方 병용이 NC/Nga 아토피 생쥐에 미치는 영향. 동의생리병리학회지. 2007;21(3): 679-687.
  20. 吳謙. 醫宗金鑑(武英殿版排印本). 北京:人民衛生出版社. 1998:907-908.
  21. 歐陽恒, 楊志波. 新編中醫皮膚病學. 북경:인민군의출판사. 2000:263, 612.
  22. 조성준. 향기치료의 기적. 서울:도서출판 宇石. 2000:120, 140, 169-171.
  23. 陳實功. 外科正宗. 北京:人民衛生出版社. 1983:269
  24. 失數道明. 韓方治療百話. 서울:동남출판사. 1991:322.
  25. 윤지연, 한재경, 김윤희. 加味淸心蓮子湯이 NC/Nga mouse의 아토피樣 피부염에 미치는 영향. 대한한방소아과학회지. 2007;21 (1):87-116.
  26. 김윤희, 한재경, 김윤희. 加味羌活散이 집먼지 진드기 추출물로 유도된 NC/Nga mouse의 아토피 피부염에 미치는 영향. 대한한방소아과학회지. 2008;22(1):69-94.
  27. 나동규, 한재경, 김윤희. 除濕胃笭湯加減이 집먼지 진드기 추출물로 유도된 NC/Nga mouse의 아토피 피부염에 미치는 영향. 대한한방소아과학회지. 2008;22(2):81-114.
  28. 여의주, 한재경, 김윤희. 아토피 피부염 동물 모델에서 아토피 크림과 紫雲膏의 병용도포가 피부염에 미치는 영향. 대전대학교 대학원 박사학위논문. 2009.
  29. 송현지, 한재경, 김윤희. 아토피樣 피부염 NC/Nga생쥐에서 加減消毒飮과 아토피크림-紫雲膏의 병용투여가 피부염에 미치는 영향. 대전대학교 대학원 박사학위논문. 2009.
  30. 이남열, 한재경, 김윤희. 아토피樣 피부염 NC/Nga 생쥐에서 滋陰除濕湯加減과 아토피 크림, 紫雲膏의 병용투여가 Th17 세포의 분화억제 및 피부염에 미치는 영향. 대전대학교 대학원 박사학위논문. 2009.
  31. 大田大學校 韓方病院. 韓方病院 處方集. 大田:韓國出版社. 2001:542.
  32. Skehan P, Storeng R, Scudiero D, Monk A, McMahon J, Visca D, Warren JT, Kennedy S, Boyd MR. New colorimetric cytotoxicity assay for anticancer drug screening. J Natl Cancer Inst. 1990;82(13):1107-1112. https://doi.org/10.1093/jnci/82.13.1107
  33. Dogru M., Katakami C., Nakagawa N., Tetsumoto K., and Yamamoto M.. Impression cytology in atopic dermatitis. Ophthalmology. 1998; 105:1478-1484. https://doi.org/10.1016/S0161-6420(98)98032-0
  34. Hoffler U., Oette K.. Comparative studies on four thrombocyte counting methods: counting in the smear preparation after Fonio, chamber counting in the phase contrast microscope, counting with the Coulter Thrombocounter, and the Technicon Auto- Counter. Z Klin Chem Klin Biochem. 1974; 12(5):198-206.
  35. Matsuda, H., N. Watanabe, G.P. Geba, J. Sperl, M. Tsudzuki, J. Hiroi.. Development of atopic dermatitis like skin lesion with IgE hyperproduction in NC/Nga mouse. Int Immunol. 1998;9:461-466.
  36. Galli SJ. Allergy. Curr. Biol. 2006;10:93-95.
  37. Yuki, Hashimoto., Iwao Arai, Yutaka Nakanishi, Takanobu Sakurai, Atsushi Nakamura.. Scratching of their skin by NC/Nga mouse leads to development of dermatitis. Life Sciences, Japan 17 March. 2004;76:783-794. https://doi.org/10.1016/j.lfs.2004.07.022
  38. K Jo hrens, I Anagnostopoulos, H Du rkop & H Stein. Different T-bet expression patteerns characterize particular reactive lymphoid tissue lesions. Histopathology. 2006;48:343- 352. https://doi.org/10.1111/j.1365-2559.2005.02305.x
  39. Danniel W. W.. A foundation for analysis in the health science. Biostics. 1983;136-146.
  40. 피부과학원색도감 편찬위원회 편, Thomas B.Fitzpatrick 외 3명공저. 皮膚과학원색도감. 서울: 정담. 1999:54.
  41. 안성구 외 2명 공저. 흔히 보는 피부질환. 서울:고려의학. 1993:6.
  42. 홍창의. 소아과학. 서울:대한교과서(주). 1994: 4-6,1066-8.
  43. Matsuda H, Watanabe N, Gregory PG et al.. Development of atopic dermatitis-like skin lesion with hyperproduction in NC/Nga mouse. Int. Immunol. 1996;9:461-466.
  44. Morita E, Kaneko S, Hiragun T et al.. Fur Mite Ext.s induce dermatitis associated with IgE hyperproduction in an inbred strain of mouse. NCKuj. J. Dermatol. Sci. 1999; 19:37-43. https://doi.org/10.1016/S0923-1811(98)00047-4
  45. Okudaira H, Hongo O, Ogita T, et al. Serum IgG and IgE antibody levels in patients with bronchial asthma, atopic dermatitis, eosinophilic granulomas of the soft tissue (Kimura's disease) and other diseases. Ann Allergy. 1983;50:51–4.
  46. Iijima OT, Takeda H, Komatsu Y, Matsumiya T, Takahashi H.. Atopic dermatitis in NCJic mouse associated with Myobia musculi infestation. Comp. Med. 2000;50:225-228.
  47. Yamaguchi T, Ono K, Shigeta S et al.. Differences in response of patient's blood cells with bronchial asthma to diverse house dust Mite Ext. allergens by histamine release assay. Jpn. J. Allergol. 1993;42: 1754-63.
  48. Leung DYM, Bieber T.. Atopic dermatitis. Lancet. 2003;361:151-160. https://doi.org/10.1016/S0140-6736(03)12193-9
  49. Kang K, Stevens SR.. Pathophysiology of atopic dermatitis. Clin. Dermatol. 2003; 21:116-121. https://doi.org/10.1016/S0738-081X(02)00361-9
  50. Ellis C, Luger T, Abeck D et al.. International Consensus Conference on Atopic Dermatitis II (ICCAD II): clinical update and current treatment strategies. Br. J. Dermatol. 2003;63:3-10.
  51. Hamid Q, Boguniewicz M, Leung DYM. Diffential in situ cytokine gene expression in acute versus chronic atopic dermatitis. J. Clin. Invest. 1994;94:870-876. https://doi.org/10.1172/JCI117408
  52. Ohmen JD, Hanifin JM, Nickoloff BJ et al.. Overexpression of IL-10 in atopic dermatitis: Contrasting cytokine patterns with delayed- type hypersensitivity reactions. J. Immunol. 1995;154: 1956-63.
  53. 박슬기, 한재경, 김윤희. NC/Nga 생쥐에서 加味淸心蓮子湯의 GATA-3 조절에 의한 항아토피 피부염 효과. 대전대학교 대학원 석사학위논문. 2009.
  54. Leung DY, Boguniewicz M, Howell MD et al.. New insights into atopic dermatitis. J. Clin. Invest. 2004;113:651-657. https://doi.org/10.1172/JCI21060
  55. Brunkow ME, Jeffery EW, Hjerrild KA, Paeper B, Clark LB, Yasayko SA, Wilkinson JE, Galas D, Ziegler SF, Ramsdell F.. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. 2001;27(1):68-73. https://doi.org/10.1038/83784
  56. 공남미, 지선영. 아토피 피부염의 양․한방적 고찰. 대한외관과학회지. 1999;12(1):241-53.
  57. 김미정, 이승연. 소아 아토피 피부염에 대한 문헌적고찰. 대한한방소아과학회지. 2002;14(2):169-83.
  58. 王伯岳, 江育仁. 中醫兒科學. 上海:上海科學技術出版社. 1984:.203-204.
  59. 전국한의과대학 본초학교수 공편저. 본초학. 서울:영림사. 2000:144, 170, 180, 186, 190, 198-199,302, 315, 326, 540.
  60. 최경임. 아로마 미용학. 서울:뷰티프라자. 2000: 30.
  61. Ouyang, W., S. H. Ranganath, K. Weindel, D. Bhattacharya, T. L. Murphy and W. C. Sha.. Inhibition of Th1 development mediated by GATA-3 through an IL-4-independent mechanism. Immunity. 1998;(9)745-755.
  62. Gavett, S. H., D. J. O'Hearn, X. Li, S. K. Huang, F. D. Finkelman and M. K. Wills.. Interleukin 12 inhibits antigen-induced airway hyperresponsiveness, inflammation, and Th2 cytokine expression in mice. J. Exp. Med. 1995;(182)1527-1536.
  63. Schreiber SL and Crabtree GR,. The mechanism of action of cyclosporin A and FK506. Immunol Today. 1992;13(4):136-142. https://doi.org/10.1016/0167-5699(92)90111-J
  64. Elena Gonzalez-Rey, Alejo Chorny and Mario Delgado. Regulation of immune tolerance by anti-inflammatory neuropeptides. Nat Rev Immunol. 2007;7:52-63. https://doi.org/10.1038/nri1984
  65. R Yagi, H Nagai, Y Iigo, T Akimoto, T Arai, M Kubo. Development of Atopic Dermatitis-Like Skin Lesions in STAT6-Deficient NC/Nga mouse. The Journal of Immunology. 2002;168:2020-7. https://doi.org/10.4049/jimmunol.168.4.2020
  66. Yang-soon Kim et. al,. Improvement of Atopic Dermatitis in NC/Nga mouse by Topical Application of CpG Phosphodiester- ODN. Int Arch Allergy Immunol. 2007; 144:315-24. https://doi.org/10.1159/000106458
  67. Tanaka A., Susumu Muto2, Kyungsook Jung1, Akiko Itai2 and Hiroshi Matsuda. Topical Application with a New NF-jB Inhibitor Improves Atopic Dermatitis in NC/NgaTnd Mice. Journal of Investigative Dermatology. 2007;127:855-863. https://doi.org/10.1038/sj.jid.5700603
  68. Ryouji Yagi, Haruko Nagai, Yutaka Iigo, Toshihiro Akimoto, Takao Arai and Masato Kubo. Development of Atopic Dermatitis-Like Skin Lesions in STAT6- Deficient NC/Nga mouse. The Journal of Immunology. 2002;168:2020-2027. https://doi.org/10.4049/jimmunol.168.4.2020
  69. Tatsuya Sasakawaa, Yasuyuki Higashib, Syozo Sakumac, Yoshitaka Hirayamaa, Yuka Sasakawaa, Yoshitaka Ohkuboa, Toshio Gotoa, Masahiro Matsumotod, Hiroshi Matsudae. Atopic Dermatitis-Like Skin Lesions Induced by Topical Application of Mite Antigens in NC/Nga mouse. Int Arch Allergy Immunol. 2001;126:239-247. https://doi.org/10.1159/000049520
  70. SENEVIRATNE S.L, A.P. BLACK, L. JONES, A.S. BAILEY and G.S. OGG. The role of skin-homing T cells in extrinsic atopic dermatitis. Oxford University Press on behalf of the Association of Physicians. 2006:1-9.
  71. Radeke HH, Ludwig RJ, Boehncke W-H. Experimental approaches to lymphocyte migration in dermatology in vitro and in vivo. Exp Dermatol. 2005;14:641–666.
  72. Wolfgang Weninger, and Ulrich H. von Andrian.. Chemokine regulation of naive T cell traffic in health and disease. Seminars in Immunology. 2003;15: 257-270. https://doi.org/10.1016/j.smim.2003.08.007