생약학회지 (Korean Journal of Pharmacognosy)

  • 제41권1호
  • /
  • Pages.21-25
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  • 2010
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  • 0253-3073(pISSN)
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  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

3T3-L1 세포에 대한 옻나무 추출물의 지방축적 억제효과

Inhibitory Effect of Heartwood of Rhus verniciflua Stokes on Lipid Accumulation in 3T3-L1 Cells

  • 김세건 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 류동영 (목포대학교 한약자원학과) ;
  • 김도국 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 고다형 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 김윤경 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 정현주 (원광대학교 약학대학 한약학과 및 원광한약연구소)
  • Kim, Se-Gun (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Rhyu, Dong-Young (Department of Medicinal Plant Resources, Mokpo National University) ;
  • Kim, Do-Kuk (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Ko, Da-Hyung (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Yun-Kyung (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Lee, Young-Mi (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Jung, Hyun-Ju (Department of Oriental Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University)
  • 투고 : 2010.01.23
  • 발행 : 2010.03.31
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초록

The MeOH extract of Rhus verniciflua heartwood inhibited lipid accumulation in 3T3-L1 adipocytes. Chromatographic methods including of silica gel, RP-18 and high-pressure liquid chromatography isolated sulfuretin and fisetin from the extract as active constituents. The isolated compounds, especially sulfuretin, strongly inhibited lipid accumulation in adipocytes. The treatment of sulfuretin and fisetin led to decreased expression of peroxisome proliferator-activated receptor-gamma ($PPAR{\gamma}$), as an important transcription factor in fat cell differentiation, which was equal to the decrease in the quercetin positive control. The presence of a hydroxyl group (C-5) in quercetin compared to fisetin, and the presence of C-2 double bonds in fisetin compared with fustin increased the inhibitory effect of lipid accumulation.

키워드

참고문헌

  1. Bray, G. A. (2006) Obesity: The disease. J. Med. Chem. 49: 4001-4007. https://doi.org/10.1021/jm0680124
  2. Friedman, J. M. (2000) Obesity in the new millennium. Nature 404: 632-634. https://doi.org/10.1038/35007504
  3. Scott, M. and Grundy (2004) Obesity, metabolic syndrome, and cardiovascular disease. J. Clin. Endocr. Metab. 89: 2595-2600. https://doi.org/10.1210/jc.2004-0372
  4. Kopelman, P. G. (2000) Obesity as a medical problem. Nature 404: 635-643. https://doi.org/10.1038/35007508
  5. Spiegelman, B. M. and Flier, J. S. (1996) Adipogenesis and Obesity: Rounding Out the Big Picture. Cell 87: 377-389. https://doi.org/10.1016/S0092-8674(00)81359-8
  6. Bravo, L. (1998) Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56: 317-333.
  7. Hertog, M. G. and Hollman, P. C. (1996) Potential health effects of the dietary flavonol quercetin. Eur. J. Clin. Nutr. 50: 63-71.
  8. Aherne, S. A. and O'Brien, N. M. (1999) Protection by the flavonoids myricetin, quercetin, and rutin against hydrogen peroxide-induced DNA damage in Caco-2 and Hep G2 cells. Nutr. Cancer 34: 160-166. https://doi.org/10.1207/S15327914NC3402_6
  9. 이창복 (2003) 대한식물도감. p. 687. 향문사, 서울.
  10. 김만조, 김갑태, 최태봉, 현정오 (1998) 기상요인과 채취시기가 옻나무 칠액채취량 및 칠액의 질에 미치는 영향. 한국자원식물학회지 11: 70-79.
  11. Choi, J. W., Yoon, B. J., Huh, K., Park, K. U., Lee, K. T. and Park, H. J. (2002) Anti-rheumatoidal effect of sulfuretin isolated from the heartwood of Rhus veniciflua in rats and Mice. Nutraceuticals & Food 7: 347-352. https://doi.org/10.3746/jfn.2002.7.4.347
  12. Anees, A. A. (2008) $^{1}H$ NMR, spectroscopic and molecular modeling studies on paramagnetic lanthanide(III)-quercetin complexes. Main Group Chemistry 7: 15-30. https://doi.org/10.1080/10241220801890072
  13. Yoshioka, T., Inokuchi, T., Fujioka, S. and Kimura, Y. (2004) Phenolic compounds and flavonoids as plant growth regulators from fruit and leaf of Vitex rotundifolia. Z. Naturforsch. 59: 509-514.
  14. V. Junior, G. M., De M. Sousa C. M., J. Cavalheiro, A., G. Lago, J. H. and H. Chaves, M. (2008) Phenolic derivatives from fruits of Dipteryx lacunifera Ducke and evaluation of their antiradical activities. Helvetica Chimica Acta 91: 2159-2167. https://doi.org/10.1002/hlca.200890233
  15. Ahn, J. Y., Lee, H. J., Kim, S. N., Park, J. H. and Ha, T. Y. (2008) The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways. Biochem. Biophys. Res. Commun. 373: 545-549. https://doi.org/10.1016/j.bbrc.2008.06.077
  16. Park, H. J., Kwon, S. H., Kim, G. T., Lee, K. T., Choi, J. H., Choi, J. and Park, K. Y. (2000) Phsicochemical and biological characteristics of flavonoids isolated from the heartwoods of Rhus verniciflua. Kor. J. Pharmacogn. 31: 345-350.
  17. Rosen, E. D. (2005) The transcriptional basis of adipocyte development. Prostaglandins Leukot Essent Fatty Acids 73: 31-34. https://doi.org/10.1016/j.plefa.2005.04.004
  18. Evans, R. M., Barish, G. D. and Wang, Y. X. (2004) PPARs and the complex journey to obesity. Nat. Med. 10: 355-361. https://doi.org/10.1038/nm1025