Properties of Bacterial Cellulose Produced in Agitated Culture Using Three Kinds of Medium

3 종류의 배지를 이용하여 진탕배양에서 생산된 Bacterial Cellulose의 물성

  • Park, Sang-Min (Department of Biomaterial Engineering, Pusan National University) ;
  • Yoon, Sang-Jun (Department of Biomaterial Engineering, Pusan National University) ;
  • Son, Hong-Joo (Department of Life Science and Environmental Biochemistry, Pusan National University) ;
  • Lee, Chung-Yeol (Department of Plant Bioscience, Pusan National University) ;
  • Kim, Hong-Sung (Department of Biomaterial Engineering, Pusan National University)
  • 박상민 (부산대학교 바이오소재공학과) ;
  • 윤상준 (부산대학교 바이오소재공학과) ;
  • 손홍주 (부산대학교 생명환경화학과) ;
  • 이충렬 (부산대학교 식물생명과학과) ;
  • 김홍성 (부산대학교 바이오소재공학과)
  • Received : 2010.06.03
  • Accepted : 2010.08.11
  • Published : 2010.08.31

Abstract

Bacterial cellulose is formed by microbial Gluconacetobacter xylinus, which produces a cellulose pellicle in its culture medium. The bacterium Gluconacetobacter sp. V6 was cultured in three types of media using an agitation method: a standard Hestrin-Schramm medium and a medium modified with either glycerol or molasses as the carbon sources. The structural properties of the cultured bacterial cellulose, such as crystallinity, viscosity and mechanical properties, were examined. Cell growth was excellent in the agitated culture with the molasses medium, and the cellulose yield was excellent in the agitated culture with the glycerol medium. The agitated culture with the molasses medium resulted in increased cell growth and cellulose yield, as well as improved mechanical properties of cellulose compared to the static culture with the molasses medium.

Keywords

Acknowledgement

Supported by : 부산대학교

References

  1. W. Czaja, D. Romanovicz, and R. Brown, "Structural Investigations of Microbial Cellulose Produced in Stationary and Agitated Culture", Cellulose, 2004, 11, 403-411. https://doi.org/10.1023/B:CELL.0000046412.11983.61
  2. R. E. Cannon and S. M. Anderson, "Biogenesis of Bacterial Cellulose", Critical Reviews in Microbiology, 1991, 17, 435-447. https://doi.org/10.3109/10408419109115207
  3. W. Cornelia and K. Dieter, "Influence of Protective Agents for Preservation of Gluconacetobacter Xylinus on Its Cellulose Production", Cellulose, 2006, 13, 485-492. https://doi.org/10.1007/s10570-005-9022-3
  4. H. I. Jung, O. M. Lee, J. H. Jeong, Y. D. Jeon, K. H. Park, H. S. Kim, W. G. An, and H. J. Son, "Production of Cellulose by Acetobacter sp. V6 Using a Cost-Effective Molasses-Corn Steep Liquor Medium", Appl Biochem Biotechnol, 2010, 162, 486-497. https://doi.org/10.1007/s12010-009-8759-9
  5. S. Kaewnopparat, K. Sansernluk, and D. Faroongsarng, "Behavior of Freezable Bound Water in the Bacterial Cellulose Produced by Acetobacter xylinum: An Approach Using Thermoporosimetry", AAPS Pharm Sci Tech, 2008, 9, 701-707. https://doi.org/10.1208/s12249-008-9104-2
  6. S. H. Moon, J. M. Park, H. Y. Chun, and S. J. Kim, "Comparisons of Physical Properties of Bacterial Celluloses Produced in Different Culture Conditions Using Saccharified Food Wastes", Biotechnol Bioprocess Eng, 2006, 11, 26-31. https://doi.org/10.1007/BF02931864
  7. S. J. Kim, H. J. Song, M. H. Chang, and C. N. Choi, "Production of Bacterial Cellulose by Pilot Scale and Its Properties", Korean J Biotechnol Bioengineering, 2007, 22, 91-96.
  8. K. Watanabe, M. Tabuchi, Y. Morinaga, and F. Yashinaga, "Structural Features and Properties of Bacterial Cellulose Produced in Agitated Culture", Cellulose, 1998, 5, 187-200. https://doi.org/10.1023/A:1009272904582
  9. S. Keshk and K. Sameshima, "Evaluation of Different Carbon Sources for Bacterial Cellulose Production", African J Biotechnol, 2005, 4, 478-482.
  10. D. Mikkelsen, B. M. Flanagan, and G. A. Dykes, "Influence of Different Carbon Sources on Bacterial Cellulose Production by Gluconacetobacter Xylinus Strain ATCC 53524", J Appl Microbiol, 2009, 107, 576-583. https://doi.org/10.1111/j.1365-2672.2009.04226.x
  11. C. Tokoh, K. Takabe, and M. Fujita, "Cellulose Synthesized by Acetobacter Xylinum in the Presence of Acetyl Glucomannan", Cellulose, 1998, 5, 249-261. https://doi.org/10.1023/A:1009211927183
  12. S. M. Park, S. J. Yoon, H. I. Jung, H. J. Son, C. Y. Lee, and H. S. Kim, "Properties of Bacterial Cellulose Cultured in Different Carbon Sources", Polymer(Korea), 2010, 34(5), in print.
  13. Y. J. Jung, "Properties of Regenerated Cellulose Films Prepared from the Tunicate Styela Clava", Bulletin of the Korean Fisheries Society, 2008, 41, 237-242.
  14. H. Yamamoto, F. Horii, and A. Hirai, "In Situ Crystallization of Bacterial Cellulose II. Influences of Different Polymeric Additives on the Formation of Celluloses $I_{\alpha}$ and $I_{\beta}$ at the Early Stage of Incubation", Cellulose, 1996, 3, 229-242. https://doi.org/10.1007/BF02228804