Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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The Effect of Autophagy to Cell Death in Nutrient-Deprived H460 Cells

영양분이 결핍된 H460 세포주에서 자가포식이 세포사멸에 미치는 영향

  • Jang, Hye-Yeon (Department of Internal Medicine, Wonkwang University College of Medicine) ;
  • Jo, Hyang-Jeong (Department of Pathology, Wonkwang University College of Medicine) ;
  • Hwhang, Ki-Eun (Department of Internal Medicine, Wonkwang University College of Medicine) ;
  • Kim, So-Young (Department of Internal Medicine, Wonkwang University College of Medicine) ;
  • Lee, Kang-Kyoo (Department of Radiation Oncology, Wonkwang University College of Medicine) ;
  • Moon, Sun-Rock (Department of Radiation Oncology, Wonkwang University College of Medicine) ;
  • Shin, Jeong-Hyun (Department of Internal Medicine, Wonkwang University College of Medicine) ;
  • Cho, Kyung-Hwa (Department of Internal Medicine, Wonkwang University College of Medicine) ;
  • Lee, Mi-Kung (Department of Thoracic Surgery, Wonkwang University College of Medicine) ;
  • Lee, Sam-Youn (Department of Thoracic Surgery, Wonkwang University College of Medicine) ;
  • Park, Soon-Ah (Department of Nuclear Medicine, Wonkwang University College of Medicine) ;
  • Park, Jong-Kun (Department of Biological Science, Wonkwang University College of Medicine) ;
  • Kim, Hui-Jung (Department of Internal Medicine, Wonkwang University College of Medicine) ;
  • Yang, Sei-Hoon (Department of Internal Medicine, Wonkwang University College of Medicine)
  • 장혜연 (원광대학교 의과대학 내과학교실) ;
  • 조향정 (원광대학교 의과대학 병리학교실) ;
  • 황기은 (원광대학교 의과대학 내과학교실) ;
  • 김소영 (원광대학교 의과대학 내과학교실) ;
  • 이강규 (원광대학교 의과대학 방사선종양학교실) ;
  • 문성록 (원광대학교 의과대학 방사선종양학교실) ;
  • 신정현 (원광대학교 의과대학 내과학교실) ;
  • 조경화 (원광대학교 의과대학 내과학교실) ;
  • 이미경 (원광대학교 의과대학 흉부외과학교실) ;
  • 이삼윤 (원광대학교 의과대학 흉부외과학교실) ;
  • 박순아 (원광대학교 의과대학 핵의학교실) ;
  • 박종군 (원광대학교 의과대학 생명과학부) ;
  • 김휘정 (원광대학교 의과대학 내과학교실) ;
  • 양세훈 (원광대학교 의과대학 내과학교실)
  • Received : 2010.05.08
  • Accepted : 2010.06.29
  • Published : 2010.08.30
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Abstract

Background: Autophagy is an important adaptive mechanism in normal development and in response to changing environmental stimuli in cancer. Previous papers have reported that different types of cancer underwent autophagy to obtain amino acids as energy source of dying cells in nutrient-deprived conditions. However, whether or not autophagy in the process of lung cancer causes death or survival is controversial. Therefore in this study, we investigated whether nutrient deprivation induces autophagy in human H460 lung cancer cells. Methods: H460, lung cancer cells were incubated in RPMI 1640 medium, and the starved media, which are BME and RPMI media without serum, including 2-deoxyl-D-glucose according to time dependence. To evaluate the viability and find out the mechanism of cell death under nutrient-deprived conditions, the MTT assay and flow cytometry were done and analyzed the apoptotic and autophagic related proteins. It is also measured the development of acidic vascular organelles by acridine orange. Results: The nutrient-deprived cancer cell is relatively sensitive to cell death rather than normal nutrition. Massive cytoplasmic vacuolization was seen under nutrient-deprived conditions. Autophagic vacuoles were visible at approximately 12 h and as time ran out, vacuoles became larger and denser with the increasing number of vacuoles. In addition, the proportion of acridine orange stain-positive cells increased according to time dependence. Localization of GFP-LC3 in cytoplasm and expression of LC-3II and Beclin 1 were increased according to time dependence on nutrient-deprived cells. Conclusion: Nutrient deprivation induces cell death through autophagy in H460 lung cancer cells.

Keywords

References

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