KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Relationship of Optical Density and Wavelength on Light Source for Activated Sludge Concentration

활성슬러지 농도에 따른 광학밀도와 광원파장의 상관관계

  • Kim, Do-Han (Ulsan Regional Innovation Agency) ;
  • Lee, Chang-Han (Department of Environmental Engineering, Catholic University of Pusan) ;
  • Wang, Bo-Kwon (Miwon Commercial Co., Ltd.) ;
  • Suh, Jung-Ho (Department of Environmental Life Chemistry, Ulsan College) ;
  • Song, Seung-Koo (Department of Chemical Engineering, Pusan National University)
  • Received : 2009.11.15
  • Accepted : 2009.12.11
  • Published : 2010.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we developed a comprehensive correlation for better prediction of the activated sludge concentration in the relationship between measurement of optical density by the Beer's law and sludge properties. Effects of activated sludge properties, which are sludge types and anaerobic storage time, show that absorbance deviation for sludge properties decreased on wavelength of light source near to 950 nm. We also developed a method to measure limitation with non-linear correlation on the measurement of high concentration by the Beer's law using a double layer probe cell and empirical equations.

Keywords

References

  1. Hong, K., R. D. Tanner, G. W. Malaney, and D. J. Wilson (1987) A spectrophotometric method for estimating the yeast cell concentration in a semi-solid state fermentation. Process Biochem. 22: 149-153.
  2. Finguerut, J., E. T. L. Guarda, and E. Camargo (1978) Influence of the growth on the spectrophotometric determination of the yeast concentration in liquid hydrocarbon fermentation. Biotech. & Bioeng. 20: 1285-1286. https://doi.org/10.1002/bit.260200812
  3. Huang, T. L., Y. W. Han, and C. D. Callihan (1971) Application of the Lowry method for determination of cell concentration in fermentation of waste cellulosics. J. Ferment. Technol. 49: 574-576.
  4. Moreira, A. R., J. A. Phillips, and A. E. Humphrey (1978) Method for determining the concentration of adsorbed protein and cell biomass in cellulose fermentations. Biotech. & Bioeng. 20: 1501-1505. https://doi.org/10.1002/bit.260200917
  5. Hysert, D. W., F. B. Knudsen, N. N. M. Morrison, G. Gheluwe, and T. Lom (1979) Application of a bioluminescence ATP assay in brewery wastewater treatment studies. Biotech. & Bioeng. 21: 1301-1314. https://doi.org/10.1002/bit.260210716
  6. Solomon, B. O. and L. E. Erickson (1983) Estimation of biomass concentration in the presence of solids for the purpose of parameter estimation. Biotech. & Bioeng. 25: 2469-2477. https://doi.org/10.1002/bit.260251015
  7. Kang, S. J., L. B. Pugh, and J. A. Borchardt (1984) ATP as a measure of active biomass concentration and inhibition in biological wastewater treatment processes. 44th Purdue Ind. Waste Conf., May 9-11. Indiana, USA.
  8. Taya, M., N. Aoki, and T. Kobayashi (1986) Estimation of microbial mass concentration based on fluorometric measurement of cell protein. J. Ferment. Technol. 64: 411-417. https://doi.org/10.1016/0385-6380(86)90028-2
  9. Kubitschek, H. E. (1969) Counting and sizing micro-organisms with the Coulter counter : Methods in Microbiology. pp. 593-610. Academic Press, London, UK.
  10. Hatch, R. T., C. Wilder, and T. W. Cadman (1979) Analysis of control of mixed cultures. Biotech. & Bioeng. 9: 25-39.
  11. Battye, F. L., W. Darling, and J. Beall (1985) A feast cell sampler for flow cytometer. Cytometry. 6: 492-495. https://doi.org/10.1002/cyto.990060516
  12. Lee, C. and H. Lim (1981) New devices for continuously monitoring the optical density of concentrated microbial cultures. Biotech. & Bioeng. 22: 639-642.
  13. Thatipamala, R., S. Rohani, and G. A. Hill (1991) Spectrophotometric method high biomass concentration measurements. Biotechnol. & Bioeng. 38: 1001-1011. https://doi.org/10.1002/bit.260380907
  14. Lima Filho, J. L. and W. M. Ledingham (1987) Continuous measurement of biomass concentration in laboratory-sacle fermentaters using LED-eletrode system. Biotechnol. Tech. 1: 145-150.
  15. Lee, C. H., Y. S. Na, D. H. Kim, S. W. Lee, and S. K. Song (2002) Changes of Physico-chemical proportics of the activated sludge with anaerobic storage time. Journal of Environmental Sciences. 11: 339-346. https://doi.org/10.5322/JES.2002.11.4.339