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Molecular Characterization of Echovirus 30-Associated Outbreak of Aseptic Meningitis in Korea in 2008

  • Choi, Young-Jin (Departments of Clinical Pathology, Soonchunhyang University Cheonan Hospital) ;
  • Park, Kwi-Sung (Chungcheongnam-Do Health and Environment Research Institute) ;
  • Baek, Kyoung-Ah (Chungcheongnam-Do Health and Environment Research Institute) ;
  • Jung, Eun-Hye (Chungcheongnam-Do Health and Environment Research Institute) ;
  • Nam, Hae-Seon (Department of Parasitology, College of Medicine, Soonchunhyang University) ;
  • Kim, Yong-Bae (Department of Preventive Medicine, College of Medicine, Soonchunhyang University) ;
  • Park, Joon-Soo (Department of Pediatrics, Soonchunhyang University Cheonan Hospital)
  • 투고 : 2009.09.03
  • 심사 : 2009.11.06
  • 발행 : 2010.03.31

초록

Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. In Korea, an outbreak of aseptic meningitis caused by echovirus type 30 (E30) occurred from May to October in 2008. In order to determine the etiologic agent, CSF and/or stool specimens from 140 children hospitalized for aseptic meningitis at Soonchunhyang University Cheonan Hospital between June and October of 2008 were tested for virus isolation and identification. E30 accounted for 61.7% (37 cases) and echovirus 6 accounted for 21.7% (13 cases) of all the human enteroviruses (HEVs) isolates (60 cases in total). For the molecular characterization of the isolates, the VP1 gene sequence of 18 Korean E30 isolates was compared pairwise using the MegAlign with 34 reference strains from the GenBank database. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the Korean strains differed from those of lineage groups A, B, C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the complete VP1 nucleotide sequences resulted in a monophyletic tree, with eight clustered lineage groups. All Korean isolates were segregated from other lineage groups, thus suggesting that the Korean strains were a distinct lineage of E30, and a probable cause of this outbreak. This manuscript is the first report, to the best of our knowledge, of the molecular characteristics of E30 strains associated with an aseptic meningitis outbreak in Korea, and their respective phylogenetic relationships.

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참고문헌

  1. Akiyoshi, K., N. Nakagawa, and T. Suga. 2007. An outbreak of aseptic meningitis in a nursery school caused by echovirus type 30 in Kobe, Japan. Jpn. J. Infect. Dis. 60: 66-68.
  2. Bailly, J. L., D. Brosson, C. Archimbaud, M. Chambon, C. Henquell, and H. Peigue-Lafeuille. 2002. Genetic diversity of echovirus 30 during a meningitis outbreak, demonstrated by direct molecular typing from cerebrospinal fluid. J. Med. Virol. 68: 558-567. https://doi.org/10.1002/jmv.10235
  3. Casas, I., G. F. Palacios, G. Trallero, D. Cisterna, M. C. Freire, and A. Tenorio. 2001. Molecular characterization of human enteroviruses in clinical samples: Comparison between VP2, VP1, and RNA polymerase regions using RT nested PCR assays and direct sequencing of products. J. Med. Virol. 65: 138-148. https://doi.org/10.1002/jmv.2013
  4. Cha, S. H. 2008. Recently prevalent infectious diseases among children: Meningitis due to enteroviral infection. J. Korean Med. Assoc. 51: 935-941. https://doi.org/10.5124/jkma.2008.51.10.935
  5. Irani, D. N. 2008. Aseptic meningitis and viral myelitis. Neurol. Clin. 26: 635-655. https://doi.org/10.1016/j.ncl.2008.03.003
  6. Kim, H. K., H. J. Kang, and K. M. Lee. 1994. Echovirus type 9 infection in an epidemic of aseptic meningitis in 1993. Korean J. Clin. Pathol. 14: 185-192.
  7. Minor, P. D. 1990. Antigenic structure of picornaviruses. Curr. Top. Microbiol. Immunol. 161: 121-154.
  8. Mirand, A., C. Henquell, C. Archimbaud, M. Chambon, F. Charbonne, H. Peigue-Lafeuille, and J. L. Bailly. 2008. Prospective identification of enteroviruses involved in meningitis in 2006 through direct genotyping in cerebrospinal fluid. J. Clin. Microbiol. 46: 87-96. https://doi.org/10.1128/JCM.01020-07
  9. Mirand, A., C. Henquell, C. Archimbaud, H. Peigue-Lafeuille, and J. L. Bailly. 2007. Emergence of recent echovirus 30 lineages is marked by serial genetic recombination events. J. Gen. Virol. 88: 166-176. https://doi.org/10.1099/vir.0.82146-0
  10. Modlin, J. F. 2000. Coxsackievirus, echoviruses, and newer enteroviruses, pp. 1904-1914. In G. L. Mandell, J. E. Bennett, and R. Dolin (eds.). Principles and Practice of Infectious Diseases, 4th Ed. Churchill Livingstone, Philadelphia, PA.
  11. Norder, H., L. Bjerregaard, L. Magnius, B. Lina, M. Aymard, and J. J. Chomel. 2003. Sequencing of 'untypable' enteroviruses reveals two new types, EV-77 and EV-78, within human enterovirus type B and substitutions in the BC loop of the VP1 protein for known types. J. Gen. Virol. 84: 827-836. https://doi.org/10.1099/vir.0.18647-0
  12. Oberste, M. S., K. Maher, M. L. Kennett, J. J. Campbell, M. S. Carpenter, D. Schnurr, and M. A. Pallansch. 1999. Molecular epidemiology and genetic diversity of echovirus type 30 (E30): Genotypes correlate with temporal dynamics of E30 isolation. J. Clin. Microbiol. 37: 3928-3933.
  13. Oberste, M. S., K. Maher, D. R. Kilpatrick, and M. A. Pallansch. 1999. Molecular evolution of the human enteroviruses: Correlation of serotype with VP1 sequence and application to picornavirus classification. J. Virol. 73: 1941-1948
  14. Oberste, M. S., K. Maher, D. R. Kilpatrick, M. R. Flemister, B. A. Brown, and M. A. Pallansch. 1999. Typing of human enteroviruses by partial sequencing of VP1. J. Clin. Microbiol. 37: 1288-1293.
  15. Oberste, M. S., W. A. Nix, K. Maher, and M. A. Pallansch. 2003. Improved molecular identification of enteroviruses by RT-PCR and amplicon sequencing. J. Clin. Virol. 26: 375-377. https://doi.org/10.1016/S1386-6532(03)00004-0
  16. Palacios, G., I. Casas, D. Cisterna, G. Trallero, A. Tenorio, and C. Freire. 2002. Molecular epidemiology of echovirus 30: Temporal circulation and prevalence of single lineages. J. Virol. 76: 4940-4949. https://doi.org/10.1128/JVI.76.10.4940-4949.2002
  17. Park, S. Y., O. S. Kwon, W. Y. Kim, W. J. Jung, S. H. Ma, K. S. Kim, et al. 2008. Epidemics of aseptic meningitis in Kyoungsangnamdo from March to October, 1997. Korean J. Pediatr. Infect. Dis. 5: 104-114.
  18. Racaniello, V. R. 2007. Picornaviridae: The viruses and their replication, p. 795-838. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (eds.). Fields Virology, 5th Ed. Lippincott Williams and Wilkins, Philadelphia, PA.
  19. Robbins, F. C., J. F. Enders, T. H. Weller, and G. L. Florentino. 1951. Studies on the cultivation of poliomyelitis viruses in tissue culture. V. The direct isolation and serologic identification of virus strains in tissue culture from patients with nonparalytic and paralytic poliomyelitis. Am. J. Hyg. 54: 286-293.
  20. Sawyer, M. H. 2002. Enterovirus infection: Diagnosis and treatment. Semin. Pediatr. Infect. Dis. 13: 40-47.
  21. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
  22. Savolainen, C., T. Hovi, and M. N. Mulders. 2001. Molecular epidemiology of echovirus 30 in Europe: Succession of dominant sublineages within a single major genotype. Arch. Virol. 146: 521–537.
  23. The Immunization Control Team, KCDC. 2008. General characteristics of viral meningitis in 2008. Public Health Weekly Report KCDC 18: 296-298.
  24. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  25. Thoelen, I., P. Lemey, I. van der Donck, K. Beuselinck, A. M. Lindberg, and M. van Ranst. 2003. Molecular typing and epidemiology of enteroviruses identified from an outbreak of aseptic meningitis in Belgium during the summer of 2000. J. Med. Virol. 70: 420-429. https://doi.org/10.1002/jmv.10412
  26. Wang, J. R., H. P. Tsai, S. W. Huang, P. H. Kuo, D. Kiang, and C. C. Liu. 2002. Laboratory diagnosis and genetic analysis of an echovirus 30-associated outbreak of aseptic meningitis in Taiwan in 2001. J. Clin. Microbiol. 40: 4439-4444. https://doi.org/10.1128/JCM.40.12.4439-4444.2002
  27. Wang, H. Y., A. Q. Xu, Z. Zhu, Y. Li, F. Ji, Y. Zhang, L. Zhang, and W. B. Xu. 2006. The genetic characterization and molecular evolution of echovirus 30 during outbreaks of aseptic meningitis. Zhonghua Liu Xing Bing Xue Za Zhi 27: 793-797.
  28. Yoshida, H., Z. Hong, T. Yoneyama, K. Yoshii, H. Shimizu, K. Ota, et al. 1999. Phylogenic analysis of echovirus type 30 isolated from a large epidemic of aseptic meningitis in Japan during 1997-1998. Japan. J. Infect. Dis. 52: 160-163.
  29. Zhao, Y. N., Q. W. Jiang, R. J. Jiang, L. Chen, and D. S. Perlin. 2005. Echovirus 30, Jiangsu Province, China. Emerg. Infect. Dis. 11: 562-567. https://doi.org/10.3201/eid1104.040995
  30. Zhao, Y. N., D. S. Perlin, S. Park, R. J. Jiang, L. Chen, Y. Chen, R. Gardiner, and Q. W. Jiang. 2006. FDJS03 isolates causing an outbreak of aseptic meningitis in China that evolved from a distinct echovirus 30 lineage imported from countries of the Commonwealth of Independent States. J. Clin. Microbiol. 44: 4142-4148. https://doi.org/10.1128/JCM.01049-06

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