한국균학회지 (The Korean Journal of Mycology)

  • 제38권1호
  • /
  • Pages.40-47
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  • 2010
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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핵 및 미토콘드리아 DNA 염기서열을 이용한 국내 Phytophthora 속의 Multi-locus phylogeny 분석

Multi-locus Phylogeny Analysis of Korean Isolates of Phytophthora Species Based on Sequence of Ribosomal and Mitochondrial DNA

  • 서문원 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 송정영 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김홍기 (충남대학교 농업생명과학대학 응용생물학과)
  • Seo, Mun-Won (Department of Applied Biology, Chungnam National University) ;
  • Song, Jeong-Young (Department of Applied Biology, Chungnam National University) ;
  • Kim, Hong-Gi (Department of Applied Biology, Chungnam National University)
  • 투고 : 2010.06.07
  • 심사 : 2010.06.17
  • 발행 : 2010.06.30
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초록

Phytophthora 속의 핵(ypt 유전자, rDNA-IGS region) 및 미토콘드리아(Cox 유전자, $\beta$-tubline 유전자, EF1A 유전자) 내에 존재하는 5가지 유전자 영역을 이용하여 국내 Phytophthora 속 14종의 유전적 다양성을 분석하였다. 국내 Phytophthora 속은 외국의 Phytophthora 속과 동일한 clade를 형성하였으나, 외국의 Phytophthora 속과 마찬가지로 본 연구에서도 분자생물학적 분류와 형태학적 분류와는 연관성을 찾기 어려웠다. 기존에 보고된 국내 P. palmivora KACC 40167 균주의 그룹이 국내에서 보고된 분류체계와 일치하지 않아 추후 재검토가 필요하였다. P. cryptogea-P. drechsleri complex group 내 국내 P. cryptogea KACC 40161 균주와 P. drechsleri KACC 40195 균주는 서로 94% 이상의 유사도를 보여 재동정이 필요하였으며, P. parasitica와 P. nicotianae간의 유사도가 99% 이상으로 나타나 이 두 종간에 통일된 종명이 요구된다. 또한 현재 분자계통학상 5그룹으로 구분된 국내 Phytophthora 속을 외국균주들과 비교하여 10개의 그룹으로 새롭게 재분류하였다. 이러한 결과들은 국내 Phytophthora 속의 유전적 다양성 연구를 위해 유용한 자료가 될 것이다.

To investigate genetic relationships either interspecies or intraspecies of 14 Korean Phytophthora species, sequence analyses of nuclear DNA (ypt gene and rDNA-IGS region) and mitochondrial DNA (Cox gene, $\beta$-tubuline gene, and EF1A gene) were performed. All of 14 Korean Phytophthora species clearly clustered into foreign isolates of each species. These Korean isolates in Phytophthora species also showed no correlation between molecular classification and morphological classification like as in case of foreigners. P. palmivora KACC 40167 reported previously from genetic groups of Phytophthora species in Korea was not consistent with the classification system, and therefore was required re-examination for the genetic group analysis. Korean isolates of P. drechsleri KACC 40195 showed very close relationship with P. cryptogea KACC 40161 above 94% bootstrap value in P. cryptogea-P. drechsleri complex group. Identification of these isolates is still unclear, because P. cryptogea and P. drechsleri were not differentiated in this study. On the other hand, it was required to unify species for these two species, since P. parasitica and P. nicotianae were clustered into a group on the level of 99 to 100% sequence homology. Comparing to the sequences of foreigners, Korean isolates were newly divided to ten groups in the phylogenic system. These results could be prepared useful informations to understand genetic diversity of Phytophthora species in Korea.

키워드

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