Mycobiology

The Korean Society of Mycology (한국균학회)
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Fruit Body Formation on Silkworm by Cordyceps militaris

  • Hong, In-Pyo (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kang, Pil-Don (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Ki-Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Nam, Sung-Hee (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Man-Young (National Academy of Agricultural Science, Rural Development Administration) ;
  • Choi, Yong-Soo (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Nam-Suk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Hye-Kyung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Kwang-Gill (National Academy of Agricultural Science, Rural Development Administration) ;
  • Humber, Richard A. (USDA-ARS Biological Integrated Pest Management Research Unit, Robert W. Holly Center for Agriculture & Health)
  • Received : 2010.03.31
  • Accepted : 2010.04.11
  • Published : 2010.06.30
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Abstract

Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a $100\;{\mu}L$ injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than $2\;{\times}\;10^5$ colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.

Keywords

References

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