The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Genetic Association of SNPs Located at PON1 Gene with Dampness and Phlegm Pattern Identification among Korean Stroke Patients

Human Paraoxonase 1(PON1)의 유전자 다형성에 따른 중풍환자의 습담 변증과의 상관성 연구

  • Lim, Ji-Hye (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Ko, Mi-Mi (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jung-Sup (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Bang, Ok-Sun (Brain Disease Research Center, Korea Institute of Oriental Medicine) ;
  • Cha, Min-Ho (Brain Disease Research Center, Korea Institute of Oriental Medicine)
  • 임지혜 (한국한의학연구원 뇌질환연구센터) ;
  • 고미미 (한국한의학연구원 뇌질환연구센터) ;
  • 이정섭 (한국한의학연구원 뇌질환연구센터) ;
  • 방옥선 (한국한의학연구원 뇌질환연구센터) ;
  • 차민호 (한국한의학연구원 뇌질환연구센터)
  • Published : 2010.12.30
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Abstract

Objective : In the present study, we investigated genetic distribution of eight single nucleotide polymorphisms of PON1 between Dampness and Phlegm and non Dampness and Phlegm pattern identification(PI) among Korean stroke patients. Materials and Methods : One hundred forty stroke subject without Dampness and Phlegm and fifty eight stroke subjects with Dampness and Phlegm were participated in this study. After informed consents, eight single nucleotide polymorphisms(SNPs) in PON1 of each subjects were identified by DNA sequencing and primer extension method and statistical analysis was performed to determine the significant difference between Dampness and Phlegm and non Dampness and Phlegm groups. Results : Among anthropometric characteristics and blood parameters, waist circumference and total cholesterol were significantly higher in Dampness and Phlegm. Among 8 SNPs of PON1, frequency of M allele and subjects with M allele in L55M SNP were significantly higher in Dampness and Phlegm group (p=0.0032 and p=0.0053, respectively) but subjects with T allele in C-2033T SNP were lower in Dampness and Phlegm group(p=0.0302). Effect of L55M and C-2033T on Dampness and Phlegm were 3.07% and 1.75%, respectively. Conclusion : Our results suggest that L55M SNP in exon and C-2033T in promoter region of PON1 maybe affect to Dampness and Phlegm pattern identification. However, further study should be carried out to find out the detailed mechanism how L55M and C-2033T can affect Dampness and Phlegm stroke patients.

Keywords

References

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