대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

흰쥐의 손상된 뇌조직에서의 유전자 발현 변화에 대한 홍화(紅花) 추출물 투여의 작용

Genome Wide Expression Analysis of the Restored Changes by Carthami Flos Extract Treatment on Rat Brain Injury

  • 김부여 (한국원자력의학원 분자암연구실) ;
  • 임세현 (고려대학교 간호대학) ;
  • 이금산 (부산대학교 한의학전문대학원) ;
  • 김형우 (부산대학교 한의학전문대학원) ;
  • 임지연 (동국대학교 대학원 의학과) ;
  • 조수인 (부산대학교 한의학전문대학원)
  • Kim, Bu-Yeo (Laboratory of Radiation Molecular Cancer, Korea Institute of Radiological and Medical Sciences) ;
  • Limb, Se-Hyun (College of Nursing, Korea University) ;
  • Lee, Guem-San (School of Korean Medicine, Pu-San National University) ;
  • Kim, Hyung-Woo (School of Korean Medicine, Pu-San National University) ;
  • Lim, Chi-Yeon (Dept. of Medicine, Graduate School, Dong-Guk University) ;
  • Cho, Su-In (School of Korean Medicine, Pu-San National University)
  • 발행 : 2010.12.30
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초록

Objectives : The source is from the flower of Carthamus tinctorius L., family Compositae. It is used in clinical medicine to promote blood circulation, remove blood stasis, promote menstruation and alleviate pain. In the present study, we investigated the genome wide analysis of Carthami Flos on the intra-cranial hemorrhage(ICH) model. Methods : ICH in rat was induced by injection of collagenase type IV and Carthami Flos extract(CFe) was administered orally. The molecular profile of cerebral hemorrhage in rat brain tissue was measured using microarray technique to identify up- or down- regulated genes in brain tissue. Results : Expression profile showed that diverse genes were up- or down-regulated by ICH induction. Administration of CFe restored the expression level of some of altered genes by ICH to normal expressional level. Interestingly, these recovered genes by CFe were involved in the same biological pathways which were significantly activated or suppressed by ICH. Conclusion : The above results might explain the therapeutic mechanism of CFe on ICH. Further, by analyzing interaction network, core genes was identified which could be key molecular targets of CFe against ICH.

키워드

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