The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effect of Yukgunja-tang on Glutamate-induced Apoptosis in C6 Glial Cells

육군자탕(六君子湯)이 Glutamate에 의한 C6 신경교세포의 Apoptosis에 미치는 영향

  • Jang, Won-Seok (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Shin, Yong-Jeen (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Ko, Seok-Jae (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Ha, Ye-Jin (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Kwon, Young-Mi (Dept. of Diagnostic Radiology, College of Oriental Medicine, Won-Kwang University) ;
  • Shin, Sun-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
  • 장원석 (원광대학교 한의과대학 내과학교실) ;
  • 신용진 (원광대학교 한의과대학 내과학교실) ;
  • 고석재 (원광대학교 한의과대학 내과학교실) ;
  • 하예진 (원광대학교 한의과대학 내과학교실) ;
  • 권영미 (원광대학교 한의과대학 영상의학과) ;
  • 신선호 (원광대학교 한의과대학 내과학교실)
  • Published : 2010.09.30
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Abstract

Objective : The water extract of Yukgunja-tang(YGJT) has been traditionally used in treatment of qi deficiency and phlegm in Oriental medicine. However, little is known about the mechanism by which YGJT protects neuronal cells from injury damages. Therefore, this study was designed to evaluate the protective effects of YGJT on C6 glial cells by glutamate-induced cell death. Methods : The present study describes glutamate, which is known as an excitatory neurotransmitter, related with oxidative damages, and YGJT, which shows protective effects against glutamate-induced C6 glial cell death. One of the main mediators of glutamate-induced cytotoxicity was known on the generation of reactive oxygen species(ROS) via activation of NADPH oxidase (NOX). The protective effects of antioxidant(NAC) and NOX inhibitor(apocynin) on the glutamate-induced C6 glial cells were determined by a MTT reduction assay. Result : YGJT inhibited glutamate-induced ROS generation via inhibition of NOX expression on glutamate-stimulated C6 glial cells. Furthermore, YGJT attenuated glutamate-induced caspase activation. These results suggest that YGJT could be a new potential candidate against glutamate-induced oxidative stress and cell death. Conclusion : These findings indicate that in C6 glial cells, ROS plays an important role of glutamate-induced cell death and that YGJT may prevent cell death from glutamate-induced cell death by inhibiting the ROS generation.

Keywords

References

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