Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Application of Bacteria Isolated from Dok-do for Improving Compressive Strength and Crack Remediation of Cement-sand Mortar

독도산 탄산칼슘형성세균에 의한 모르타르 균열보수와 압축강도 증진

  • Park, Sung-Jin (School of Life Sciences, Kyungpook National University) ;
  • Lee, Na-Young (School of Architecture & Architectural Engineering, Kyungpook National University) ;
  • Kim, Wha-Jung (School of Architecture & Architectural Engineering, Kyungpook National University) ;
  • Ghim, Sa-Youl (School of Life Sciences, Kyungpook National University)
  • 박성진 (경북대학교 생명과학부) ;
  • 이나영 (경북대학교 건축토목공학부) ;
  • 김화중 (경북대학교 건축토목공학부) ;
  • 김사열 (경북대학교 생명과학부)
  • Received : 2010.05.04
  • Accepted : 2010.06.07
  • Published : 2010.06.28
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Abstract

This study shows an application of microbiologically induced carbonate precipitate for strength improvement and crack remediation of cement-sand mortar. Seven calcium carbonate-forming bacteria (CFB) were isolated from Dok-do and partially identified by DNA sequence analysis of the 16s rRNA gene. Crystal aggregates were apparent around the bacterial colonies grown on an agar medium. These strains showed strain specific $CaCO_3$ precipitation on urea-$CaCl_2$ medium. Among 7 isolates, Arthrobacter nicotinovorans KNUC601, Microbacterium resistens KNUC602, Agrobacterium tumefaciens KNUC603, Exiguobacterium acetylicum KNUC604, and Bacillus thuringiensis KNUC606 showed a repairing of artificial forced cracks in cement-sand mortar. Compressive strength of cement-sand mortar consolidated with Stenotrophomonas maltophilia KNUC605 was increased around 14.07% compared with that of negative control.

본 연구는 탄산칼슘형성세균을 이용하여 시멘트-모래 모르타르의 압축강도증진 및 균열보수의 응용에 연구의 목적이 있다. 독도로부터 분리한 7가지의 탄산칼슘형성세균을 16S rDNA 염기서열을 이용하여 동정했다. 고체배지상의 콜로니 주변부에 형성되는 광물결정을 확인했다. Urea-$CaCl_2$ 배지에서 형성되는 광물의 모양은 종 특이적인 것을 확인했다. Arthrobacter nicotinovorans KNUC601, Microbacterium resistens KNUC602, Agrobacterium tumefaciens KNUC603, Exiguobacterium acetylicum KNUC604, 및 Bacillus thuringiensis KNUC606균주는 인위적으로 만든 모르타르 균열부위를 메우는 것을 확인했다. Stenotrophomonas maltophilia KNUC605가 혼입된 시멘트-모래 모르타르는 음성대조구에 비해 14.7%정도 강도가 증가됐다.

Keywords

References

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