Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Physicochemical Characterization and NMR Assignments of Ginsenosides Rb1, Rb2, Rc, and Rd Isolated from Panax ginseng

  • Cho, Jin-Gyeong (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University) ;
  • Lee, Min-Kyung (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University) ;
  • Lee, Jae-Woong (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Park, Hee-Jung (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University) ;
  • Lee, Dae-Young (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University) ;
  • Lee, Youn-Hyung (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Yang, Deok-Chun (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and Department of Oriental Medicinal Materials & Processing, Kyung Hee University)
  • Received : 2010.02.08
  • Accepted : 2010.03.31
  • Published : 2010.06.30
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Abstract

The fresh ginseng roots were extracted with aqueous methanol, and the obtained extracts were partitioned using ethyl acetate, n-butanol, and water, successively. The repeated silica gel and octadecyl silica gel column chromatogaraphy for n-butanol fraction afforded four diol ginseng saponins, ginsenosides $Rb_1$, $Rb_2$, $R_c$, and Rd. The physicochemical, spectroscopic, and chromatographic characteristics of these ginsenosides were measured and compared with those reported in the literature. Some of the peak assignments in previously published $^1H$- and $^{13}C$-nuclear magnetic resonance (NMR) spectra were inaccurate. This study employed two-dimensional NMR experiments, including $^1H-^1H$ correlation spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity, to determine exact peak assignments.

Keywords

References

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