Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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The Effect of the Bujeonghangam-tang Extract on Hepatocellular Carcinogenesis and Hepatic Cirrhosis Induced by Diethylnitrosarnine and CCl4 in Rats

부정항암탕(扶正抗癌湯) 추출액이 Diethylnitrosamine과 CCl4로 유발된 흰쥐의 간암 형성 및 간경화에 미치는 영향

  • Moon, Young-Ho (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Won, Jin-Hee (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Moon, Goo (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Heo, Rae-Kyong (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Seung, Kee-Moon (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Lee, In-Young (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Jang, Myung-Joon (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Kwon, So-Yeon (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Won-kwang University) ;
  • Yu, Deok-Seon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine. Won-kwang University)
  • 문영호 (원광대학교 한의과대학 내과학교실) ;
  • 원진희 (원광대학교 한의과대학 내과학교실) ;
  • 문구 (원광대학교 한의과대학 내과학교실) ;
  • 허래경 (원광대학교 한의과대학 내과학교실) ;
  • 성기문 (원광대학교 한의과대학 내과학교실) ;
  • 이인영 (원광대학교 한의과대학 내과학교실) ;
  • 장명준 (원광대학교 한의과대학 내과학교실) ;
  • 권소연 (원광대학교 한의과대학 내과학교실) ;
  • 유덕선 (원광대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.05.17
  • Accepted : 2010.06.11
  • Published : 2010.06.30
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Abstract

Objective : Bujeonghangam-tang(BH) has been used for cure of tumor as a traditional medicine. This study was carried out to clarify the effect of BH extract on hepatocellular carcinogenesis and hepatic cirrhosis induced by Diethylnitrosamine(DENA) and $CCl_4$ in Rats. Method : Experimental groups were divided into two, 8th and 12th week groups, and subdivided into four; normal group(Nor), cirrhosis and hepatic cancer inducing control group(Con), and BH extract 320 mg/kg/day(BHA) or 640 mg/kg/day(BHB) administered groups to Con. Results: In the 8th week group: The body weight decreased significantly in Con compared with the Nor. The activities of transaminase, alkaline phosphatase(ALP), and lactacte dehydrogenase(LDH) were significantly increased(p<0.05) in the Con compared with Nor, but decreased in the BHA and BHB compared with Con. Alpha fetoprotein(AFP) were the most increased in the Con compared to BHA and BHB. The results of light microscopical observation, a number of hepatocytes were damaged in the Con compared with Nor and BH extract administerd groups. The number of hepatic p53 positive cells was reduced in the BH extract administered groups. According to the electron microscopical observation, hepatocarcinoma cells were observed distinctly in the Con compared with BH extract administered groups. In the 12 weeks group: The results of body were similar to 8th week groups. The activities of transaminase and ALT were significantly increased(p<0.05) in the Con compared with Nor. LDH was significantly(p<0.05) increased in the Con compared with Nor but significantly (p<0.05) decreased in the BHB. Alpha fetoprotein(AFP) were the most increased in the Con among ex perimental groups. The activities of superoxide dismutase(SOD) were significantly (p<0.05) increased in the Con, but the activities of catalase were not increased(p<0.05) compared with Nor. The number of hepatic p53 positive cells was increased in the Con. The results of electron microscopical observation were similar to 8th week groups. Conclusion : These results suggest that ad ministration of BH extract suppress or retard DENA and $CCl_4$-induced hepatocellular carcinogenesis and hepatic cirrhosis in rats.

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References

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