사상체질의학회지 (Journal of Sasang Constitutional Medicine)

  • 제22권4호
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  • Pages.65-76
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  • 2010
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  • 1226-4075(pISSN)
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  • 2287-786X(eISSN)
사상체질의학회 (The Society Of Sasang Constitutional Medicine)
권호 표지

형방도적산(荊防導赤散)이 급성코카인 투여로 인해 유도된 흰쥐의 행동량과 c-Fos 발현에 미치는 영향

Effect of Hyeongbangdojeok-san on Acute Cocaine-induced Behavioral Effect and Immediate Early Gene Expression in Rats.

  • 서지용 (대구한의대학교 한의학과 사상체질과) ;
  • 최애련 (대구한의대학교 한의학과 사상체질과) ;
  • 구덕모 (대구한의대학교 한의학과 사상체질과)
  • Seo, Ji-Yong (Dept. of Sasang Constitutional Medicine, college of Oriental Medicine, Deagu Haany Univ.) ;
  • Choi, Ae-Ryun (Dept. of Sasang Constitutional Medicine, college of Oriental Medicine, Deagu Haany Univ.) ;
  • Koo, Deok-Mo (Dept. of Sasang Constitutional Medicine, college of Oriental Medicine, Deagu Haany Univ.)
  • 투고 : 2010.08.13
  • 심사 : 2010.08.27
  • 발행 : 2010.12.31
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초록

1. Objectives The present study was designed to investigate the effect of Soyangin Hyeongbangdojeok-san(HBDJS) on acute cocaine-induced behavior effect and gene expression in the rat brain. 2. Methods Experimental animals were composed of saline(SAL), cocaine(COC), HBDJS + COC, HBDJS + SAL group. Rats received HBDJS(100, 200 mg/kg, p.o.) 1 h prior to cocaine hydrochloride(20 mg/kg, i.p.) treatment respectively. After cocaine injection, locomotor activity and rearing were measured in a rectangular container equipped with a video camera above the center of the floor for 60 min. In addiction, c-Fos expression in the rat brain was detected using immunohistochemistry 2 h after cocaine injection. And the effect of HBDJS on acute cocaine-induced pERK, pElk, pCREB upstream of c-Fos expression was detected using western blotting and immunohistochemistry 15 min after cocaine challenge. 3. Results The present results show that HBDJS at dose of 200 mg/kg attenuated cocaine-induced both locomotor activity and rearing. Also HBDJS at dose of 200 mg/kg significantly decreased c-Fos expression in the rat brain(nucleus accumebns and striatum). However HBDJS at dose of 200 mg/kg have no effect on cocaine-induced pERK, pCREB, pElK-1 expression. HBDJS is c-Fos expression through ERK-independent pathway. 4. Conclusions. These results suggest that HBDJS may be effective in suppressing the reinforcing effects of cocaine.

키워드

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