Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of Integron-Associated Multi-Drug Resistance of Acinetobacter baumannii Isolated in Korea

국내에서 분리된 Acinetobacter baumannii의 Integron과 연관된 다제내성 분석

  • Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Choi, Ji-Hye (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Park, Eun-Jin (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Suh, In-Won (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Son, Seung-Yeol (Department of Microbiology and Institute of Basic Sciences, Dankook University)
  • 김성환 (단국대학교 첨단과학대학 미생물학과 및 기초과학연구소) ;
  • 최지혜 (단국대학교 첨단과학대학 미생물학과 및 기초과학연구소) ;
  • 박은진 (단국대학교 첨단과학대학 미생물학과 및 기초과학연구소) ;
  • 서인원 (단국대학교 첨단과학대학 미생물학과 및 기초과학연구소) ;
  • 손승렬 (단국대학교 첨단과학대학 미생물학과 및 기초과학연구소)
  • Received : 2010.07.30
  • Accepted : 2010.09.16
  • Published : 2010.09.30
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Abstract

Acinetobacter baumannii 1625, a clinical isolate identified by Vitek and 16S rDNA sequence, showed an extended resistance to most ${\beta}$-lactams including imipenem, kanamycin, gentamicin, tobramycin, and cephalosporins of the third and fourth generations, and produced metallo-${\beta}$-lactamase (MBL) of IMP-1 type which is rare in Korea. The isolate contained a class 1 integron of about 2.5 kb in size and the integron included accA4 (aminoglycoside resistance gene), $bla_{IMP-1}$ (carbapenem resistance gene), and $bla_{OXA-2}$ (extended-spectrum ${\beta}$-lactam resistance gene) gene cassettes in order. The coexistence of IMP-1 type and OXA-2 type ${\beta}$-lactamase gene cassettes in an integron has not been reported in Korea. The transformed integron rendered the E. coli transformant resistant more than eight folds against imipenem, ampicilin, piperacillin, cefazolin, cefoperazone, and aztreonam comparing to the reference strain. This study clearly showed that the extended multi-drug resistance of A. baumannii 1625 was mainly due to the integron.

임상분리 균주인 A. baumannii 1625는 imipenem (carbapenem 계열)을 포함한 대부분의 ${\beta}$-lactam계열의 항생제들과 kanamycin, gentamicin, tobramycin 및 제 3세대와 4세대 cephalosporin 계열의 항생제들에 광범위한 내성을 나타내었고, 한국에서는 드문 IMP-1형 metallo-${\beta}$-lactamase (MBL)를 생성하는 균주임이 확인되었다. A. baumannii 1625는 약 2.5 kb 크기의 class 1 integron을 갖고 있었으며, 이 integron내에 aminoglycoside계열 내성 유전자인 accA4, carbapenem 계열 내성 유전자인 $bla_{IMP-1}$, 광범위한 ${\beta}$-lactam 내성 유전자인 $bla_{OXA-2}$ 유전자 cassette들이 차례대로 위치해 있음을 확인하였는데, 이것은 IMP-1형과 OXA-2형 ${\beta}$-lactamase의 유전자를 같은 integron내에 동시에 갖는 새로운 배열 및 구조로서 이전에 국내에서 보고된 바 없는 것이다. 이 2.5 kb 크기의 integron을 항생제 내성이 없는 E. coli에 형질전환 시켰을 때, imipenem, ampicillin, piperacillin, cefazolin, cefoperazone, aztreonam 등의 항생제들에 대하여 8배 이상 증가된 내성정도를 보였다. 이는 A. baumannii 1625의 integron이 다제내성을 부여하는 기능을 하고 있음을 보여준다.

Keywords

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