Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Different Dietary Vitamin E Levels on Growth Performance, Non-specific Immune Responses, and Disease Resistance against Vibrio anguillarum in Parrot Fish (Oplegnathus fasciatus)

  • Galaz, German Bueno (Departamento de Ciencias del Mar, Universidad Artuto Prat) ;
  • Kim, Sung-Sam (Department of Marine Life Science, Jeju National University) ;
  • Lee, Kyeong-Jun (Marine and Environmental Research Institute, Jeju National University)
  • Received : 2009.09.22
  • Accepted : 2010.01.05
  • Published : 2010.07.01
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Abstract

We report nutritional physiology and non-specific immune responses of vitamin E in parrot fish for the first time. This study aimed to investigate the essentiality and requirements in diets based on growth performances, non-specific immune responses and a challenge test against Vibrio angullarum. Six casein-gelatin based semi-purified diets were formulated to contain six graded levels of DL-${\alpha}$-tocopheryl acetate (${\alpha}$-TA) at 0, 25, 50, 75, 100 and 500 mg/kg diet (designated as E0, E25, E50, E75, E100 and E500, respectively) and fed to triplicate groups of juvenile parrot fish for 12 weeks. The analyzed dietary concentrations of vitamin E were 0, 38, 53, 87, 119 and 538 mg/kg diet for E0, E25, E50, E75, E100 and E500, respectively. At the end of the feeding trial, growth performance and feed utilization of fish fed the E25 were significantly higher compared to that of fish fed the other diets. Liver ${\alpha}$-tocopherol concentration was significantly increased with an increase in dietary ${\alpha}$-TA in a dose dependent manner. No apparent clinical signs of vitamin E deficiency and mortality were observed in fish fed the basal diet for 12 weeks. Among the immune responses assayed, phagocytic (NBT assay) and myeloperoxidase activities were significantly increased with increment of dietary ${\alpha}$-TA levels. During the challenge test with V. anguillarum, E75, E100, and E500 diets resulted in higher survivals than E0, E25 and E50 diets. The findings of this study suggest that parrot fish require exogenous vitamin E and the optimum dietary level could be approximately 38 mg ${\alpha}$-TA/kg diet for normal growth and physiology. Dietary ${\alpha}$-TA concentration over 500 mg/kg could be required to enhance the nonspecific immune responses and improve the resistance of juvenile parrot fish against V. anguillarum.

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References

  1. Anderson, D. P. and A. K. Siwicki. 1995. Basic haematology and serology for fish health programs. Pages 185-202 in M. Shariff, J. R. Arthur and R. P. Subasinghe, editors. Fish Health Section, Asian Fisheries Society, Manila, Philippines.
  2. Anderson, D. P. 1992. Immunostimulants, adjuvants, and vaccine carriers in fish: application to aquaculture. Annu. Rev. Fish Dis. 2:281-307. https://doi.org/10.1016/0959-8030(92)90067-8
  3. AOAC (Association of Official Analytical Chemists). 2000. Official methods of analysis of AOAC International, 17th edition. AOAC International, Gaithersburg, Maryland, USA.
  4. Bai, S. C. and D. M. III. Gatlin. 1993. Dietary vitamin E concentration and duration of feeding affect tissue $\alpha$-tocopherol concentrations of channel catfish (Ictalurus punctatus). Aquaculture 113:129-135. https://doi.org/10.1016/0044-8486(93)90346-Z
  5. Bai, S. C. and K. J. Lee. 1998. Different levels of dietary DL-$\alpha$-tocopheryl acetate affect the vitamin E status of juvenile Korean rockfish, Sebastes schlegeli. Aquaculture 161:405-414. https://doi.org/10.1016/S0044-8486(97)00288-3
  6. Baker, R. T. M. and S. J. Davies. 1996. Changes in tissue $\alpha$-tocopherol status and degree of lipid peroxidation with varying $\alpha$-tocopheryl acetate inclusion in diets for the African catfish. Aquacult. Nutr. 2:71-79. https://doi.org/10.1111/j.1365-2095.1996.tb00011.x
  7. Blazer, V. S. 1992. Nutrition and disease resistance in fish. Annu. Rev. Fish Dis. 2:309-323. https://doi.org/10.1016/0959-8030(92)90068-9
  8. Chen, R., R. Lochmann, A. Goodwin, K. Praveen, K. Dabrowski, and K. J. Lee. 2004. Effects of dietary vitamins C and E on alternative complement activity, hematology tissue composition, vitamin concentrations and response to heat stress in juvenile golden shiner (Notemigonus crysoleucas). Aquaculture 242:553-569. https://doi.org/10.1016/j.aquaculture.2004.09.012
  9. Clem, L. W., R. C. Sizemore, C. F. Ellsaesser and N. W. Miller. 1985. Monocytes as accessory cells in fish immune responses. Dev. Comp. Immunol. 9:803-809. https://doi.org/10.1016/0145-305X(85)90046-1
  10. Cort, W. M., T. S. Vicente, E. H. Waysek and B. D. Williams. 1983. Vitamin E content of feedstuffs determined by high performance liquid chromatographic fluorescence. J. Agric. Food Chem. 31:1330-1333. https://doi.org/10.1021/jf00120a045
  11. Cowey, C. B., J. W. Adron and A. Youngson. 1983. The vitamin E requirement of rainbow trout (Salmo gairdneri) given diets containing polyunsaturated fatty acids derived from fish oil. Aquaculture 30:85-93. https://doi.org/10.1016/0044-8486(83)90154-0
  12. Dalmo, R. A., K. Ingebrightsen and J. Bogwald. 1997. Nonspecific defense mechanisms in fish, with particular reference to the reticuloendothelial system (RES). J. Fish Dis. 20:241-273. https://doi.org/10.1046/j.1365-2761.1997.00302.x
  13. Eo, J. and K. J. Lee. 2008. Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer, Takifugu rubripes. Fish Shellfish Immunol. 25:611-616. https://doi.org/10.1016/j.fsi.2008.08.009
  14. Fevolden, S. E., K. H. Roed and B. Gjerde. 1994. Genetic components of post-stress cortisol and lysozyme activity in Atlantic salmon; correlation to disease and resistance. Fish Shellfish Immunol. 4:507-519. https://doi.org/10.1006/fsim.1994.1045
  15. Fletcher, M. P., M. E. Gershwin, C. L. Keen and L. S. Hurley. 1988. Trace element deficiencies and immune responsiveness in human and animal models. Pages 215-239 in R. J. Chandra, editor. Contemporary Issues in Clinical Nutrition. Vol. II. Nutrition and Immunology. Alan R. Liss, Inc, New York, USA.
  16. Garling, D. L. J. and R. P. Wilson. 1976. Optimum dietary protein to energy ratio for channel catfish fingerlings, Ictalurus punctatus. J. Nutr. 106:1368-1375.
  17. Gatlin, D. M. III., S. C. Bai and M. C. Erickson. 1992. Effects of dietary vitamin E and synthetic antioxidants on composition and storage quality of channel catfish, Ictalurus punctatus. Aquaculture 106:323-332. https://doi.org/10.1016/0044-8486(92)90264-L
  18. Halver, J. E. 2002. The vitamins. Fish Nutrition (Ed. J. E. Halver and R. W. Hardy) pp. 61-141. Academic Press, San Diego, California, USA.
  19. Hung, S. S. O., C. Y. Cho and S. J. Slinger. 1981. Effect of oxidized fish oil, DL-$\alpha$-tocopheryl acetate and ethoxyquin supplementation on the vitamin E nutrition of rainbow trout (Salmo gairdneri) fed practical diets. J. Nutr. 111:648-657.
  20. Hung, S. S. O., C. Y. Cho and S. J. Slinger. 1980. Measurement of oxidation in fish oil and its effect on vitamin E nutrition of rainbow trout (Salmo gairdneri). Can. J. Fish. Aquat. Sci. 37:1248-1253. https://doi.org/10.1139/f80-160
  21. Kettunen, A. and K. T. Fjalestad. 2006. Resistanse to vibriosis in Atlantic cod (Gadus morhua L.): First challenge test results. Aquaculture 258:263-269. https://doi.org/10.1016/j.aquaculture.2006.04.042
  22. Kiron, V., J. Puangkaew, K. Ishizka, S. Satoh and T. Watanabe. 2004. Antioxidant status and nonspecific immune responses in rainbow trout (Oncorhynchus mykiss) fed two levels of vitamin E along with three lipid sources. Aquaculture 234:361-379. https://doi.org/10.1016/j.aquaculture.2003.11.026
  23. Ko, G. Y., S. J. Lim, S. S. Kim, D. H. Oh and K. J. Lee. 2008. Effects of dietary supplementation of Scoria on growth and protein digestibility in juvenile parrot fish Oplegnathus fasciatus and olive flounder Paralichthys olivaceus. J. Aquacult. 21:133-138.
  24. Kumari, J. and P. K. Sahoo. 2005. Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish Clasrias batrachus. Fish Shellfish Immunol. 19:307-316. https://doi.org/10.1016/j.fsi.2005.01.008
  25. Kumari, J. and P. K. Sahoo. 2006. Dietary $\beta$-1,3 glucan potentiates innate immunity and disease resistance of Asian catfish, Clarias batrachus (L.). J. Fish Dis. 29:95-101. https://doi.org/10.1111/j.1365-2761.2006.00691.x
  26. Kumari, J., T. Swain and P. K. Sahoo. 2003. Dietary bovine lactoferrin induces changes in immunity level and disease resistance in Asian catfish Clarias batrachus. Vet. Immunol. Immunopathol. 94:1-9. https://doi.org/10.1016/S0165-2427(03)00065-5
  27. Lall, S. P., G. Oliver, J. A. Hines and H. W. Ferguson. 1988. The role of vitamin E in nutrition and immune response of Atlantic salmon (Salmo salar). Aquacult. Assoc. Can. Bull. 88:76-78.
  28. Landolt, M. 1989. The relationship between diet and the immune response of fish. Aquaculture 79:193-206. https://doi.org/10.1016/0044-8486(89)90461-4
  29. Lee, J. Y., S. K. Chun and S. I. Park. 1988. Serotyping of Vibrio anguillarum isolated from cultured marine fishes. J. Fish Pathol. 1:45-50.
  30. Lee, S. K., Y. S. Kim, C. Y. Liang and Y. H. Song. 2003. Effects of dietary vitamin E supplementation on color stability, lipid oxidation and reducing ability of Hanwoo (Korean cattle) beef during retail display. Asian-Aust. J. Anim. Sci. 16(10):1529-1534. https://doi.org/10.5713/ajas.2003.1529
  31. Li, P., X. Wang and D. M. III. Gatlin. 2008. RRR-$\alpha$-Tocopheryl succinate is a less bioavailable source of vitamin E than allrac-$\alpha$-tocopheryl acetate for red drum, Sciaenops ocellatus. Aquaculture 280:165-169. https://doi.org/10.1016/j.aquaculture.2008.04.027
  32. Lin, C. F., J. I. Gray, A. Asghar, D. J. Buckley, A. M. Booren and C. J. Flegal. 1989. Effects of dietary oils and $\alpha$-tocopherol supplementation on lipid composition and stability of broiler meat. J. Food Sci. 54:1457-1460. https://doi.org/10.1111/j.1365-2621.1989.tb05134.x
  33. Lin, Y. F. and S. J. Chang. 2006. Effect of dietary vitamin E on growth performance and immune response of breeder chickens. Asian-Aust. J. Anim. Sci. 19(6):884-891. https://doi.org/10.5713/ajas.2006.884
  34. Lin, Y. H. and S. Y. Shiau. 2005. Dietary vitamin E requirement of grouper, Epinephelus malabaricus, at two lipid levels, and their effects on immune responses. Aquaculture 248:235-244. https://doi.org/10.1016/j.aquaculture.2005.04.020
  35. Lohakare, J. D., S. H. Lee and B. J. Chae. 2006. Effect of dietary fat-soluble vitamins on growth performance and nutrient digestibility in growing pigs. Asian-Aust. J. Anim. Sci. 19(4):563-567. https://doi.org/10.5713/ajas.2006.563
  36. Lovell, R. T., T. Miyazaki and S. Rabegnator. 1984. Requirements for $\alpha$-tocopherol by channel catfish fed diets low in polyunsaturated triglycerides. J. Nutr. 114:894-901.
  37. Lygren, B., B. Hjeltnes and R. Waagbo. 2001. Immune response and disease resistance in Atlantic salmon (Salmo salar L.) fed three levels of dietary vitamin E and the effect of vaccination on the liver status of antioxidants vitamins. Aquac. Int. 9:401-411. https://doi.org/10.1023/A:1020509308400
  38. Murai, T. and J. W. Andrews. 1974. Interactions of dietary $\alpha$-tocopherol, oxidized menhaden oil and ethoxyguin on channel catfish (Ictalurus punctatus). J. Nutr. 104:1416-1431.
  39. NRC (National Research Council). 1993. Nutrient requirements of fish. National Academy of Sciences, National Academy Press, Washington DC, USA.
  40. Ortuno, J., M. A. Esteban and J. Meseguer. 2003. The effect of dietary intake of vitamins C and E on the stress response of gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol. 14:145-156. https://doi.org/10.1006/fsim.2002.0428
  41. Panda, N., H. Kaur and T. K. Mohanty. 2006. Reproductive performance of dairy buffaloes supplemented with varying levels of vitamin E. Asian-Aust. J. Anim. Sci. 19(1):19-25.
  42. Pham, M. A. and K. J. Lee. 2007. Effects of dietary Cheongkukjang on liver superoxide dismutase activity of parrot fish Oplegnathus fasciatus. J. Aquacult. 20:132-139.
  43. Poston, H. A., G. F. Combs and L. Leibovitz. 1976. Vitamin E and selenium interrelations in the diets of Atlantic salmon (Salmo salar): gross, histological and biochemical deficiency signs. J. Nutr. 106:892-904.
  44. Poston, H. A. and D. L. Livingston. 1969. Effects of massive doses of dietary vitamin E on fingerling brook trout. Pages 9-12 in H. A. Poston, and D. L. Livingston, editors. Fish. Res. Bull. Cortland Hatchery Report, New York, Courtland, USA.
  45. Puangkaew, J., V. Kiron, T. Somamoto, N. Okamoto, S. Satoh, T. Takeuchi and T. Watanabe. 2004. Nonspecific immune response of rainbow trout (Oncorhynchus mykiss Walbaum) in relation to different status of vitamin E and highly unsaturated fatty acids. Fish Shellfish Immunol. 16:25-39. https://doi.org/10.1016/S1050-4648(03)00028-7
  46. Pulsford, A. L., M. Crampe, A. Langston and P. J. Glynn. 1995. Modulatory effects of disease, stress, copper, TBT and vitamin E on the immune system of flatfish. Fish Shellfish Immunol. 5:631-643. https://doi.org/10.1016/S1050-4648(95)80046-8
  47. Quade, M. J. and J. A. Roth. 1997. A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet. Immunol. Immunopathol. 58:239-248. https://doi.org/10.1016/S0165-2427(97)00048-2
  48. Roem, A. J., C. C. Kohler and R. R. Stickney. 1990. Vitamin E requirements of the blue tilapia, Oreochromis aureus, in relation to dietary lipid levels. Aquaculture 87:155-164. https://doi.org/10.1016/0044-8486(90)90272-O
  49. Sahoo, P. K. and S. C. Mukherjee. 2001. Effects of dietary $\beta$-1,3 glucan on immune responses and disease resistance of healthy and aflatoxin $B_1$-induced immunocompromised rohu (Labeo rohita Hamilton). Fish Shellfish Immunol. 11:683-695. https://doi.org/10.1006/fsim.2001.0345
  50. Samanta, A. K., R. S. Dass, M. Rawat, S. C. Mishra and U. R. Mehra. 2006. Effect of dietary vitamin E supplementation on serum $\alpha$-tocopherol and immune status of crossbred calves. Asian-Aust. J. Anim. Sci. 19(4):500-506. https://doi.org/10.5713/ajas.2006.500
  51. Sankaran, K. and S. Gurnani. 1972. On the variation in catalytic activity of lysozyme in fishes. Indian J. Biochem. Biophys. 9:162-165.
  52. Swain, P., S. Dash, P. K. Sahoo, P. Routray, S. K. Sahoo, S. D. Gupta, P. K. Meher and N. Sarangi. 2007. Non-specific immune parameters of brood Indian major carp Labeo rohita and their seasonal variations. Fish Shellfish Immunol. 22:38-43. https://doi.org/10.1016/j.fsi.2006.03.010
  53. Toyoda, Y. 1985. Study on quantitative requirements of fat-soluble vitamins in yellowtail. Master's thesis. Kochi University, Nankoku, Japan.
  54. Wilson, R. P., P. R. Bowser and W. E. Poe. 1984. Dietary vitamin E requirement of fingerling channel catfish. J. Nutr. 114:2053-2058.
  55. Woodall, A. N., L. M. Ashley, J. E. Halver, H. S. Olcott and J. Van Der Veen. 1964. Nutrition of salmonoid fishes: XIII. The $\alpha$-tocopherol requirement of chinook salmon. J. Nutr. 84:125-135.

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