Journal of Microbiology and Biotechnology

  • 제20권12호
  • /
  • Pages.1672-1676
  • /
  • 2010
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Identification of Streptomyces sp. KH29, Which Produces an Antibiotic Substance Processing an Inhibitory Activity Against Multidrug-Resistant Acinetobacter baumannii

  • 투고 : 2010.07.20
  • 심사 : 2010.09.13
  • 발행 : 2010.12.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The Actinomycete strain KH29 is antagonistic to the multidrug-resistant Acinetobacter baumannii. Based on the diaminopimelic acid (DAP) type, and the morphological and physiological characteristics observed through the use of scanning electron microscopy (SEM), KH29 was confirmed as belonging to the genus Streptomyces. By way of its noted 16S rDNA nucleotide sequences, KH29 was found to have a relationship with Streptomyces cinnamonensis. The production of an antibiotic from this strain was found to be most favorable when cultured with glucose, polypeptone, and yeast extract (PY) medium for 6 days at $27^{\circ}C$. The antibiotic produced was identified, through comparisons with reported spectral data including MS and NMR as a cyclo(L-tryptophanyl-L-tryptophanyl). Cyclo(L-Trp-L-Trp), from the PY cultures of KH29, was seen to be highly effective against 41 of 49 multidrug-resistant Acinetobacter baumannii. Furthermore, cyclo(L-Trp-L-Trp) had antimicrobial activity against Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Saccharomyces cerevisiae, Aspergillus niger, and Candida albicans, However, it was ineffective against Streptomyces murinus.

키워드

참고문헌

  1. Aswapokee, N., S. Tiengrim, B. Charoensook, and K. Sangsiriwut. 1998. Antimicrobial resistant pattern of Acinetobacter sp. J. Infect. Dis. Antimicrob. Agents 15: 43-48.
  2. Baraibar, J., H. Correa, D. Mariscal, M. Gallego, J. Valles, and J. Rello. 1997. Risk factors for infection by Acinetobacter baumannii in intubated patients with nosocomial pneumonia. Chest 112: 1050-1054. https://doi.org/10.1378/chest.112.4.1050
  3. Bergogne-Berezin, E. and K. J. Towner. 1996. Acinetobacter spp. as nosocomial pathogens: Microbiological, clinical, and epidemiological features. Clin. Microbiol. Rev. 9: 148-165.
  4. Bello, H., G. Gonzalez, M. Dominguez, R. Zemelman, A. Garcia, and S. Mella. 1997. Activity of selected beta-lactams, ciprofloxacin, and amikacin against different Acinetobacter baumannii biotypes from Chilean hospitals. Diagn. Microbiol. Infect. Dis. 28: 183-186. https://doi.org/10.1016/S0732-8893(97)00074-6
  5. Getchell-White, S. I., L. G. Donowitz, and D. H. Groschel. 1989. The inanimate environment of an intensive care unit as a potential source of nosocomial bacteria: Evidence for long survival of Acinetobacter calcoaceticus. Infect. Control Hosp. Epidemiol. 10: 402-407. https://doi.org/10.1086/646061
  6. Graz, M., A. Hunt, H. Jamie, G. Grant, and P. Milne. 1999. Antimicrobial activity of selected cyclic dipeptides. Pharmazie 54: 772-775.
  7. Hanberger, H., J. A. Garcia-Rodriguez, M. Gobernado, H. Goossens, L. E. Nilsson, and M. J. Struelens. 1999. Antibiotic susceptibility among aerobic Gram-negative bacilli in intensive care units in 5 European countries. J. Am. Med. Assoc. 281: 67-71. https://doi.org/10.1001/jama.281.1.67
  8. Jeanmougin, F. and G. Orsini. 1998. Multiple sequence alignment with Clustal X. Trends Biochem. Sci. 23: 403-405. https://doi.org/10.1016/S0968-0004(98)01285-7
  9. Lim, D. S., S. K. Yoon, M. S. Lee, W. H. Yoon, and C. H. Kim. 1996. Isolation and identification of Streptoverticillium sp. NA-4803 producing antifungal substance. Kor. J. Appl. Microbiol. 24: 664-670.
  10. Manikal, V. M., D. Landman, G. Saurina, E. Oydna, H. Lal, and J. Quale. 2000. Endemic carbapenem-resistant Acinetobacter species in Brooklyn, New York: Citywide prevalence, interinstitutional spread, and relation to antibiotic usage. Clin. Infect. Dis. 31: 101-106. https://doi.org/10.1086/313902
  11. Park, A. J. and H. R. Hong. 2003. Evaluation of methods for detection of antimicrobial resistance of Acinetobacter baumannii to imipenem. Korean J. Lab. Med. 23: 388-394.
  12. Pridham, T. G., C. W. Hesseltine, and R. G. Benedict. 1958. A guide for classification of Streptomycetes according to selected group placement of strains in morphological sections. Appl. Microbiol. 6: 52-79.
  13. Richards, M. J., J. R. Edwards, D. H. Culve, and R. P. Gaynes. 1999. Nosocomial infections in medical intensive care units in the United States. National Nosocomial Infections Surveillance System. Crit. Care Med. 27: 887-892. https://doi.org/10.1097/00003246-199905000-00020
  14. Rhee, K. H. 2002. Isolation and characterization of Streptomyces sp. KH-614 producing anti-VRE (vancomycin-resistant enterococci) antibiotics. J. Gen. Appl. Microbiol. 48: 664-670.
  15. Sader, H. S., R. N. Jones, A. C. Gales, P. Winokur, K. C. Kugler, M. A. Pfaller, and G. V. Doern. 1998. Antimicrobial susceptibility patterns for pathogens isolated from patients in Latin American medical centers with a diagnosis of pneumonia: Analysis of results from the SENTRY Antimicrobial Surveillance Program (1997). SENTRY Latin America Study Group. Diagn. Microbiol. Infect. Dis. 32: 289-301. https://doi.org/10.1016/S0732-8893(98)00124-2
  16. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for constructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
  17. Shirling, E. B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces sp. Int. J. Syst. Bacteriol. 16: 313-340. https://doi.org/10.1099/00207713-16-3-313
  18. Somerville, D. A. and W. C. Noble. 1970. A note on the Gramnegative bacilli of human skin. Rev. Eur. Etud. Clin. Biol. 15: 669-671.
  19. Song, J. S., J. H. Park, C. H. Kim, Y. W. Kim, W. J. Lim, I. Y. Kim, and H. I. Chang. 1999. Characterization of the replication region of the Enterococcus faecalis plasmid p703/5. J. Microbiol. Biotechnol. 9: 91-97.
  20. Vincent, J. L., D. J. Bihari, and P. M. Suter. 1995. The prevalence of noscomial infection in intensive care units in Europe. J. Am. Med. Assoc. 274: 639-644. https://doi.org/10.1001/jama.274.8.639
  21. Williams, S. T. and F. L. Davies. 1967. Use of a scanning electron microscope for the examination of Actinomycetes. J. Gen. Microbiol. 48: 171-177. https://doi.org/10.1099/00221287-48-2-171
  22. Williams, S. T., M. E. Sharpe, J. G. Holt, G. E. Murray, D. J. Brener, N. R. Krieg, et al. 1989. Bergey's Manual of Systematic Bacteriology, Vol. 4, Williams & Wilkins, Baltimore.
  23. Yamada, K. and K. Komagata. 1970. Taxonomic studies on coryneform bacteria. II. Principle amino acids in the cell wall and their taxonomic significance. J. Gen. Appl. Microbiol. 16: 103-113. https://doi.org/10.2323/jgam.16.1_103

피인용 문헌

  1. Cyclic dipeptides from lactic acid bacteria inhibit proliferation of the influenza a virus vol.51, pp.6, 2010, https://doi.org/10.1007/s12275-013-3521-y
  2. A Novel Alkaloid from Marine-Derived Actinomycete Streptomyces xinghaiensis with Broad-Spectrum Antibacterial and Cytotoxic Activities vol.8, pp.10, 2010, https://doi.org/10.1371/journal.pone.0075994
  3. Cyclic Dipeptides from Lactic Acid Bacteria Inhibit the Proliferation of Pathogenic Fungi vol.52, pp.1, 2010, https://doi.org/10.1007/s12275-014-3520-7
  4. Drug Resistance and the Prevention Strategies in Food Borne Bacteria: An Update Review vol.9, pp.3, 2010, https://doi.org/10.15171/apb.2019.041
  5. Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil vol.9, pp.4, 2010, https://doi.org/10.3390/antibiotics9040176