Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Characterization of Atypical Shigella flexneri Isolated in Korea

  • Hong, Sa-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Choi, Yeon-Hwa (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Choo, Yun-Ae (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Choi, Young-Woon (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Choi, Seon-Young (Molecular Bacteriology Laboratory, International Vaccine Institute) ;
  • Kim, Dong-Wook (Molecular Bacteriology Laboratory, International Vaccine Institute) ;
  • Lee, Bok-Kwon (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health) ;
  • Park, Mi-Sun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea National Institute of Health)
  • Received : 2010.05.12
  • Accepted : 2010.07.02
  • Published : 2010.10.28
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Abstract

Three types of serotypically atypical Shigella flexneri isolates were collected between 2007 and 2008 from Korean patients at the Korea National Institute of Health (NIH). These atypical isolates were characterized and compared with serologically typical S. flexneri. The first grouping of 11 atypical isolates displayed agglutination only with polyB antiserum and exhibited no reaction with any typing or grouping sera (PolyB:un). The second group of 3 isolates displayed reactions with typing sera IV, but also did not bind with any grouping sera (IV:un). The third group of 14 isolates exhibited a plural agglutination pattern, reacting with typing sera II, and two grouping sera (II:(3)4,7(8)). Amongst these atypical isolates, isolates belonging to IV:un and II:(3)4,7(8) exhibited greater antibiotic resistance, in particular to ampicillin, streptomycin, and trimethoprim-sulfamethoxazole, than typical S. flexneri strains. Furthermore, all II:(3)4,7(8) strains harbored integrons. This study suggests that these multiple antibiotic-resistant atypical S. flexneri are new subserotypes of S. flexneri that await further serological classification.

Keywords

References

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