Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Synergistic Effect of Methanol Extract of Salvia Miltiorrhiza and Antibiotics against Dental Caries Pathogens

치아우식증유발세균에 대한 단삼 메탄올추출물과 항생제와의 병용효과

  • Jang, Keoun-Ae (Department of Dental Hygiene, MaSan University) ;
  • Kim, Hye-Young (Department of Dental Hygiene, College of Natural Sciences, Dongeui University)
  • Received : 2010.04.26
  • Accepted : 2010.06.21
  • Published : 2010.09.28
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Abstract

Salvia miltiorrhiza Bunge (S. miltiorrhiza) is a traditional Korean medicine that is commonly used for the treatment of inflammatory diseases such as edema, arthritis, and hepatitis. The present study investigated the antimicrobial activity of methanol (MeOH) extract of S. miltiorrhiza roots against oral bacteria using broth the microdilution method and the checkerboard and time-kill methods evaluated the synergistic effects of treatment with antibiotics. The MeOH extract was demonstrated as a higher antibacterial activity (MICs, 8 to $64\;{\mu}g/mL$; MBCs, 16 to $64\;{\mu}g/mL$) against all tested oral bacteria. Additionally, the extract was observed to have a synergistic effect with ampicillin or gentamicin. A time-kill study evaluating the effects of the extract indicated that the extract treatment in combination with ampicillin or gentamicin showed rapid bactericidal activity. The results suggest that MeOH extract of S. miltiorrhiza could be employed as a natural antibacterial agent against dental caries.

단삼(Salvia miltiorrhiza Bunge)은 우리나라에서 전통적으로 부종, 관절염, 간염 등과 같은 염증질환의 치료에 사용해왔다. 본 연구에서는 단삼 메탄올 추출물을 이용하여 구강미생물에 대한 항균활성을 확인하였다. 그 결과 메탄올추출물은 실험 되어진 모든 구강미생물에서 강한 항균활성을 나타내었다(MICs, 8 to $64\;{\mu}g/mL$; MBCs, 16 to $64\;{\mu}g/mL$). 추가적으로 ampicillin이나 gentamicin과의 병용투여서 최소억제농도(MIC)와 최소살균농도(MBC)가 감소하는 병용효과를 나타내었다. 더불어 메탄올추출물 단독 사용시 보다 항생제와 병용투여시 빠른 사멸효과를 보였다. 결론적으로 단삼 메탄올추출물이 치아우식을 유발하는 많은 세균들에 대한 항균효과가 뛰어남을 확인 할 수 있었으며, 안정성이 높은 천연 치아우식예방제로서의 개발가능성을 확인하였다.

Keywords

References

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