The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Cryopreservation of Scapharca broughtonii (Schrenck) Sperm

피조개, Scapharca broughtonii (Schrenck) 정자의 동결보존

  • Rha, Sung-Ju (Division of Marine Technology, Chonnam National University) ;
  • Han, Kyeong-Ho (Division of Marine Technology, Chonnam National University) ;
  • Choi, Myeong-Rak (Division of Biotechnology and Chemical Engineering, Chonnam National University) ;
  • Kho, Kang-Hee (Division of Marine Technology, Chonnam National University)
  • Received : 2010.09.03
  • Accepted : 2010.11.13
  • Published : 2010.12.31
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Abstract

This study was conducted to investigate protocol standardization for spermatozoa cryopreservation of the Scapharca broughtonii (Schrenck). Among the freezing rates, freezing at a height of 2 cm above liquid nitrogen surface for 5 minute gave higher activity and survival rate. Among the various diluents, Ringer's solution was the best for S. broughtonii sperm cryopreservation. The suitability of cryoprotectants dimethylsulfoxide (DMSO), dimethylacetamide (DMA), glycerol and methanol were tested against three freezing rates. DMSO gave significantly higher activity and survival rates than others.

피조개, Scapharca broughtonii (Schrenck) 정자의 동결보존 위해 동결높이에 따른 예비동결 (0, 2, 4, 6 cm), 희석제 (150 mM NaCl, 300 mM glucose, Ringer's solution)와 동해방지제(glycerol, dimethylsulfoxide (DMSO), dimethylacetamide (DMA), methaol) 에 따른 효과를 정자의 운동성과 생존율로 비교하였다. 피조개 정자 동결보존에 있어 예비동결은 액체질소표면으로부터 2 cm가 가장 효과적이었으며, 희석제로는 Ringer's solution이 동해방지제로는 DMSO 10% 농도가 가장 적합하였다.

Keywords

References

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