The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Effects of water temperature and salinity on the formation of prezoosporangia and zoosporangia of the protozoan parasite, Perkinsus olseni, isolated from the Manila clam Ruditapes philippinarum on the west coast of Korea

퍼킨서스편모충 (Perkinsus olseni) 의 휴면포자와 유주자 형성에 수온과 염분이 미치는 영향

  • Kim, Hyon-Joong (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Bang, In-Seok (Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University) ;
  • Park, Kyung-Il (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 김현중 (군산대학교 해양과학대학 수산생명의학과) ;
  • 방인석 (호서대학교 자연과학대학 생명과학과, 기초과학연구소) ;
  • 박경일 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2010.06.15
  • Accepted : 2010.07.19
  • Published : 2010.09.30
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Abstract

The genus Perkinsus are parasitic protozoans that cause massive inflammatory responses in infected marine shellfish worldwide. This ultimately leads to great economic losses. This study examined the effects of water temperature and salinity on the formation of prezoosporangia and zoosporangia in order to understand the ecology of the pathogens. The induction of prezoosporangia from trophozoites occurred readily at higher water temperatures (20 and $30^{\circ}C$) and they had larger diameters than those incubated at lower temperatures (4 and $10^{\circ}C$). The formation of zoospores in prezoosporangia was also strongly influenced by water temperature and salinity; prezoosporangia exposed to water temperatures of 20 and $30^{\circ}C$ and salinities of 20 and 30 ppt had high rates of zoosporulation, while no or very low rates of zoosporulation were observed at temperatures below $10^{\circ}C$ or salinity below 10 ppt. Our data will be useful for the development of strategies to counter P. olseni proliferation in Korean waters.

퍼킨서스편모충 P. olseni는 우리나라 바지락의 대부분이 감염되어 있으며 극심한 염증을 유발하는 우리나라 바지락의 대표적 기생충이다. 본 연구에서는 퍼킨서스편모충의 생태학적 특성을 조사하고자 영양체에서 휴면포자로 전환되는 과정과 유주자를 형성하는 과정에서 수온과 염분이 미치는 영향을 조사하였다. 조사결과 영양체에서 휴면포자로의 유도는 수온이 높을수록 유도율이 높고 휴면포자의 직경이 커지는 현상이 관찰되었다. 또한 유주자 형성은 고수온, 고염분의 환경에서 잘 형성됨으로써 $10^{\circ}C$ 이하에서는 유주자 발생이 이루어지 않으며, 10 ppt에서는 유주자 형성이 급격히 감소하고 5 ppt에서는 유주자 발생이 이루어지지 않음이 확인되었다. 이러한 퍼킨서스편모충의 생태학적 정보는 이 질병의 구제 대책 수립에 중요한 정보를 제공할 수 있을 것으로 기대된다.

Keywords

References

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