Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Evaluation of Resistance in Hot Pepper Germplasm to Phytophthora Blight on Biological Assay

생물검정을 통한 고추 유전자원의 역병저항성 평가

  • Kim, Jeong-Soon (National Agrobiodiversity Center, National Academy of Agricultural Science) ;
  • Kim, Won-Il (Microbial Safety Division, National Academy of Agricultural Science) ;
  • Jee, Hyeong-Jin (Organic Farming Technology Division, National Academy of Agricultural Science) ;
  • Gwang, Jae-Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science) ;
  • Kim, Chung-Kon (National Agrobiodiversity Center, National Academy of Agricultural Science) ;
  • Shim, Chang-Ki (National Agrobiodiversity Center, National Academy of Agricultural Science)
  • 김정순 (국립농업과학원 농업유전자원센터) ;
  • 김원일 (국립농업과학원 유해생물과) ;
  • 지형진 (국립농업과학원 유기농업과) ;
  • 곽재균 (국립농업과학원 농업유전자원센터) ;
  • 김정곤 (국립농업과학원 농업유전자원센터) ;
  • 심창기 (국립농업과학원 농업유전자원센터)
  • Received : 2010.04.28
  • Accepted : 2010.06.01
  • Published : 2010.10.31
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Abstract

Phytophthora blight of pepper is the most economically important disease in the world cultivation regions. We investigated the phytophthora blight resistance of 300 accessions of Korean landrace of hot pepper germplasms collected from 83 local regions. The disease incidence rate was checked from 7 days to 28 days at an interval of 7 days after inoculation under greenhouse conditions. Among 300 accessions, the disease incidence rate of phytophthora blight of 67 accessions of pepper germplasm was more than 60.1%, while no disease was observed in 37 accessions at 7 days after inoculation. At 28 days after inoculation, five and eleven accessions of pepper germplasm were resistance and moderate resistance to $P.$ $capsici$, respectively. Two hundred forty four susceptible accessions (81.3%) of pepper were scored as having more than 60.1% of disease incidence of phytophthora blight. This result suggests that five candidate pepper germplasm might be used as breeding resources for the phytophthora blight resistance breeding program. Also, further genetic studies should be carried out to verify this result, with the overall focus of providing information on important characteristics of pepper germplasm.

역병은 세계적으로 고추에 발생하는 병 중 가장 문제시 되는 병해로 국내 83개 지역에서 채집한 재래종 고추 유전자원 중 300개를 선발하여 공시하였다. 파종 후 온실에서 70일간 육묘하였고 공시한 역병균의 유주자낭 농도를 $10^5/mL$으로 하여 주당 5mL 관주접종 후, 습실 처리하였고 1주 간격으로 4주간 이병주율을 조사하였다. 접종 7일 후 이병주율을 조사하였더니, 공시한 300개 고추 자원 중 67개 자원에서 이병주율이 60.1% 이상으로 높은 감수성을 보였으며, 37개 자원은 접종 후 7일까지 전혀 발병하지 않았다. 역병균 접종 28일 후, 공시한 300점의 고추자원 중 244개 자원은 60% 이상의 이병주율을 보여 역병에 대해 감수성을 나타내었으며, 11개 자원은 20% 미만의 이병주율을 보여 중도저항성을 보였고, 5개 자원은 전혀 발병하지 않아 저항성을 나타내었다. 5개 자원의 저항성 고추유전자원은 역병저항성 고추 품종 육종에 유용한 중간모본으로 사용될 것으로 생각된다. 본 실험의 정밀도를 높이기 위해 분자마커를 활용한 추가실험이 진행된다면 고추유전자원의 역병 저항성에 대한 중요한 정보를 제공할 수 있을 것으로 본다.

Keywords

References

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