Bulletin of the Korean Chemical Society

  • 제31권6호
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  • Pages.1573-1576
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  • 2010
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Mutational Analysis of the Metal-binding Sites of Peroxide Sensor PerR

  • Won, Young-Bin (Department of Life Science and Research Center for Natural Sciences, Hanyang University) ;
  • Ji, Chang-Jun (Department of Life Science and Research Center for Natural Sciences, Hanyang University) ;
  • Cho, Ju-Hyun (Department of Biology, Research Institute of Life Science, Gyeongsang National University) ;
  • Lee, Jin-Won (Department of Life Science and Research Center for Natural Sciences, Hanyang University)
  • 투고 : 2010.04.08
  • 심사 : 2010.04.27
  • 발행 : 2010.06.20
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초록

Bacillus subtilis PerR is a metal-dependent peroxide-sensing transcription factor which uses metal-catalyzed histidine oxidation for peroxide-sensing. PerR contains two metal binding sites, one for structural $Zn^{2+}$ and the other for the regulatory/peroxide-sensing metal. Here we investigated the effect of mutations at both the structural and regulatory metal binding sites on the oxidation of either H37 or H91, two of the peroxide-sensing ligands. All four serine substitution mutants at the structural $Zn^{2+}$ site (C96S, C99S, C136S and C139S) exhibited no detectable oxidation at histidine residues. Two of the alanine substitution mutants at regulatory metal site (H37A and D85A) exhibited selective oxidation preferentially at the H91-containing tryptic peptide, whereas no oxidation was detected in the other mutants (H91A, H93A and D104A). Our results suggest that the cysteine residues coordinating structural $Zn^{2+}$ are essential for peroxide sensing by PerR, and that the C-terminal regulatory metal binding site composed of H91, H93 and D104 can bind $Fe^{2+}$, providing a possible explanation for the peroxide sensing mechanisms by PerR.

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참고문헌

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피인용 문헌

  1. PerR Is a Hypersensitive Hydrogen Peroxide Sensor using Iron-mediated Histidine Oxidation vol.290, pp.33, 2015, https://doi.org/10.1074/jbc.M115.664961
  2. Bacillus licheniformis Contains Two More PerR-Like Proteins in Addition to PerR, Fur, and Zur Orthologues vol.11, pp.5, 2016, https://doi.org/10.1371/journal.pone.0155539
  3. 155 vol.108, pp.6, 2018, https://doi.org/10.1111/mmi.13956
  4. Metalloregulatory proteins: Metal selectivity and allosteric switching vol.156, pp.2, 2010, https://doi.org/10.1016/j.bpc.2011.03.010