대한암한의학회지 (Journal of Korean Traditional Oncology)
- 제15권1호
- /
- Pages.37-45
- /
- 2010
- /
- 1975-244X(pISSN)
- /
- 2765-4850(eISSN)
보정방독탕 에탄올 추출물의 안전성에 대한 연구
Study on the Safety of Bojungbangdocktang Ethanol Extract
- 이은옥 (경희대학교 한의과대학) ;
- 이효정 (경희대학교 한의과대학) ;
- 이효정 (경희대학교 한의과대학) ;
- 정수진 (경희대학교 한의과대학) ;
- 최도영 (경희대학교 한의과대학) ;
- 정희재 (경희대학교 한의과대학) ;
- 안규석 (경희대학교 한의과대학) ;
- 강종구 (충북대학교 수의과대학) ;
- 김성훈 (경희대학교 한의과대학)
- Lee, Eun-Ok (College of Oriental Medicine, Kyunghee University) ;
- Lee, Hyo-Jeong (College of Oriental Medicine, Kyunghee University) ;
- Lee, Hyo-Jung (College of Oriental Medicine, Kyunghee University) ;
- Jeong, Soo-Jin (College of Oriental Medicine, Kyunghee University) ;
- Choi, Do Young (College of Oriental Medicine, Kyunghee University) ;
- Jung, Hee-Jae (College of Oriental Medicine, Kyunghee University) ;
- Ahn, Kyoo-Seok (College of Oriental Medicine, Kyunghee University) ;
- Kang, Jong-Gu (College of Veternerary Medicine, Chungbuk University) ;
- Kim, Sung-Hoon (College of Oriental Medicine, Kyunghee University)
- 투고 : 2010.12.07
- 심사 : 2010.12.23
- 발행 : 2010.12.28
초록
Bojungbangdocktang (BJBDT), a formula of eight Oriental herbs, is a modified herbal prescription of Bangdoktang and Bojungbangamtang. Recently, BJBDT was demonstrated to inhibit angiogenesis induced by vascular endothelial growth factor in human umbilical vein endothelial cells, enhance hematopoiesis and protect cisplatin-induced cytotoxicity in normal MCF-10A breast cells. Nevertheless, there is no safety study of BJBDT before clinical trial so far. Thus, in the current study, we investigated the toxicity about ethanol-extracted BJBDT. Male and female Spraque Dawley (SD) rats were given orally by BJBDT at 250, 500, and 1000 mg/kg for 4 weeks. Mortality, clinical signs and measured change of body weight, food consumption and water consumption were observed. In addition, we performed ophthalmologic, urinary, hematological, blood serum biochemical and histopathological examination. Any general toxicity was not found in BJBDT treated group. Also, there were no significant differences in the parameters such as body weight, food consumption and water consumption, a lot of urine and blood factor levels except HCT, MCHC, Ca, TG, Glucose and T-Bilirubin level compared with control group. Although HCT was elevated and TG was decreased in male rats, and MCHC, Glucose and T-Bilirubin were elevated and Ca and HCT were decreased in female rats, these were within normal ranges. Finally, we determined that maximum tolerated dose (MTD) was 1000 mg/kg and no observed adverse effect level (NOAEL) was 500 mg/kg. Taken together, these results demonstrated that BJBDT is very safe to SD rats.