유치 수복물에 따른 주변 법랑질의 탈회 저항성: QLF를 이용한 연구

RESISTANCE TO DEMINERALIZATION OF ENAMEL OF PRIMARY TEETH ACCORDING TO RESTORATIONS: IN VITRO STUDY USING QLF

  • 권해숙 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 현홍근 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 김영재 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 김정욱 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 장기택 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 김종철 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 한세현 (서울대학교 대학원 치의학과 소아치과학교실) ;
  • 이상훈 (서울대학교 대학원 치의학과 소아치과학교실)
  • Kwon, Hae-Sook (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Hyun, Hong-Keun (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Young-Jae (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Jung-Wook (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Jang, Ki-Taeg (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Chong-Chul (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Hahn, Se-Hyun (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Lee, Sang-Hoon (Department of Pediatric Dentistry, School of Dentistry, Seoul National University)
  • 발행 : 2010.02.26

초록

본 연구에서는 유치 수복물의 불소 방출 여부에 따른 주변 법랑질의 탈회 저항성과 재광화 효과를 살펴보고자 하였다. 건전 유전치 48개를 16개씩 임의로 세 군으로 나누어, $Filtek^{TM}$ Z250(1군), F2000(2군), $Ketac^{TM}$ N100(3군)을 사용하여 제조사의 지시에 따라 충전하였다. 3일 동안 인공 우식 병소를 유발한 후, 14일 동안 인공 타액에 담가 재광화를 유발하였다. Quantitative light-induced fluorescence(QLF)를 이용하여 실험 단계와 시간에 따른 무기질 소실량(${\Delta}Q$)의 변화를 관찰한 결과는 다음과 같다. 1. 탈회가 일어난 인공 우식 병소의 ${\Delta}Q$ 값은 3군, 2군, 1군 순으로 작게 나타났고, 이 중 3군은 1군에 비해 유의한 탈회 저항성을 보였다. 2. 1군, 2군, 3군 모두 재광화 1일 후부터 초기 인공 우식 병소의 ${\Delta}Q$ 값과 비교 시 유의한 차를 보였으며, 관찰 기간 동안 지속적인 증가 양상을 나타내었다. 3. 재광화 속도를 의미하는 ${\Delta}$(${\Delta}Q$)/일 값은 각 군에서 처음 1일 동안 유의성 있게 크게 나타났으며, 그 이후 급속히 감소하였다. 4. 수복 재료에 따른 재광화 정도는 각 군 간에 유의한 차이가 없었다.

The objective of this in vitro study was to detect and monitor demineralization and remineralization of primary teeth according to restorative materials using quantitative light-induced fluorescence (QLF). A single bur hole was drilled on the each sound forty eight primary anterior teeth, and the specimens were divided into three groups. The cavity was restored with $Filtek^{TM}$ Z250(Group 1), F2000(Group 2), $Ketac^{TM}$ N100(Group 3) following the manufacturer's instructions. The teeth were subjected to the demineralizing buffer for 3 days, and then subjected to a remineralizing buffer for 14 days. The change of mineral loss(${\Delta}Q$) according to the stages was evaluated by QLF and the following results were obtained: 1. When demineralization was done, ${\Delta}Q$ was increased as follows. : Group 1 ($-110.79\;{\pm}\;27.77$) < Group 2 ($-104.84\;{\pm}\;28.95$) < Group 3 ($-90.16\;{\pm}\;21.87$) : Resistance to demineralization was statistically significant in Group 3. 2. There was a statistically significant increase in ${\Delta}Q$ of all groups since 1st day of remineralization 3. The rate of remineralization, ${\Delta}$(${\Delta}Q$)/day, showed significant high value in each group on the 1st day then decreased rapidly. 4. There was no statistically significant difference in the degree of remineralization among restorative materials.

키워드

참고문헌

  1. National Institute of Dental and Craniofacial Research. Oral health in America: a report of the Surgeon General-executive summary. Rockville (MD): National Institutes of Health, Department of Health and Human Services; 2000.
  2. Kagihara LE, Niederhauser VP, Stark M : Assessment, management, and prevention of early childhood caries. J Am Acad Nurse Pract, 21:1-10, 2009. https://doi.org/10.1111/j.1745-7599.2008.00367.x
  3. Mjor IA : Placement and replacement of restoration. Oper Dent, 6:49-54, 1981.
  4. Maryniuk GA, Kaplan SH : Longevity of restorations: survey results of dentists' estimates and attitudes. J Am Dent Assoc, 112:39-45, 1986.
  5. Featherstone JD : Prevention and reversal of dental caries: role of low level fluoride. Community Dent Oral Epidemiol, 27:31-40, 1999. https://doi.org/10.1111/j.1600-0528.1999.tb01989.x
  6. Hicks J, Garcia-Godoy F, Flaitz C : Biological factors in dental caries enamel structure and the caries process in the dynamic process of demineralization and remineralization (part 2). J Clin Pediatr Dent, 28:119-124, 2004.
  7. Hicks J, Garcia-Godoy F, Flaitz C : Biological factors in dental caries: role of remineralization and fluoride in the dynamic process of demineralization and remineralization (part 3). J Clin Pediatr Dent, 28:203-214, 2004.
  8. Arends J, ten Bosch JJ : Demineralization and remineralization evaluation techniques. J Dent Res, 71 Spec No:924-928, 1992.
  9. Reynolds EC : Remineralization of enamel subsurface lesions by casein phophopeptide-stabilized calcium phophate solutions. J Dent Res, 76:1587-1595, 1997. https://doi.org/10.1177/00220345970760091101
  10. Benson PE, Pender N, Higham SM : An in situ caries model to study demineralization during fixed orthodontics. Clin Orthod Res, 2:143-153, 1999.
  11. Ogaard B, Rolla G : The in vivo orthodontic banding model for vital teeth and the in situ orthodontic banding model for hard-tissue slabs. J Dent Res, 71 Spec No:832-835, 1992.
  12. Fontana M, Li Y, Dunipace AJ, et al. : Measurement of enamel demineralization using microradiography and confocal microscopy. A correlation study. Caries Res, 30:317-325, 1996. https://doi.org/10.1159/000262337
  13. Stookey GK : Quantitative light fluorescence: a technology for early monitoring of the caries process. Dent Clin North Am, 49:753-770, 2005. https://doi.org/10.1016/j.cden.2005.05.009
  14. 황규선, 김종수, 유승훈 : Digital imaging fiber-optic trans- illumination과 laser fluorescence를 이용한 평활면 초기우식증의 재광화에 관한 비교 연구. 대한소아치과학회지, 34:183-191, 2007.
  15. Caliskan Yanikoglu F, Ozturk F, Hayran O, et al. : Detection of natural white spot caries lesions by an ultrasonic system. Caries Res, 34:225-232, 2000. https://doi.org/10.1159/000016595
  16. 김효석, 김왕근, 이창섭, 이상호 : 초기우식병소에 대한 레이저 fluorescence의 광학적 탐지감도. 대한소아치과학회지, 26:109-118, 1999.
  17. Stookey GK : Optical methods-quantitative light fluorescence. J Dent Res, 83 Spec No C:C84-88, 2004. https://doi.org/10.1177/154405910408301S17
  18. Pretty IA, Pender N, Edgar WM, Higham SM : The in vitro detection of early enamel de- and re-mineralization adjacent to bonded orthodontic cleats using quantitative light-induced fluorescence. Eur J Orthod, 25:217-223, 2003. https://doi.org/10.1093/ejo/25.3.217
  19. Higham SM, Pretty IA, Edgar WM, Smith PW : The use of in situ models and QLF for the study of coronal caries. J Dent, 33:235-241, 2005. https://doi.org/10.1016/j.jdent.2004.10.016
  20. Pretty IA, Edgar WM, Higham SM : The effect of ambient light on QLF analysis. J Oral Rehabil, 29:369-373, 2002. https://doi.org/10.1046/j.1365-2842.2002.00914.x
  21. Takagi S, Liao H, Chow LC : Effect of a low-fluoride- content, two-component rinse on fluoride uptake and on de- and remineralization of enamel lesions: an in vitro study. Caries Res, 35:223-228, 2001. https://doi.org/10.1159/000047460
  22. Forsten L : Fluoride release and uptake by glassionomers and related materials and its clinical effect. Biomaterials, 19:503-508, 1998. https://doi.org/10.1016/S0142-9612(97)00130-0
  23. Suljak JP, Hatibovic-Kofman S : A fluoride releaseadsoprtion- release system applied to fluoridereleasing restorative materials. Quintessence Int, 27:635-638, 1996.
  24. Forsten L : Fluoride release and uptake by glass ionomers. Scand J Dent Res, 99:241-245, 1991.
  25. Wiegand A, Buchalla W, Attin T : Review on fluoride- releasing restorative materials-Fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater, 23:343-362, 2007. https://doi.org/10.1016/j.dental.2006.01.022
  26. ten Cate JM, Featherstone JD : Physicochemical aspects of fluoride-enamel interaction. In: Fejerskov O, Ekstrand J, Bust BA, editors. Fluoride in dentistry. Copenhagen: Munksgaard; 252-272, 1996.
  27. Bell A, Creanor SL, Foye RH, et al. : The effect of saliva on fluoride release by a glass-ionomer filling material. J Oral Rehabil, 26:407-412, 1999. https://doi.org/10.1046/j.1365-2842.1999.00389.x
  28. Itota T, Nakabo S, Torii Y, et al. : Effect of fluoridereleasing liner on demineralized dentin. Quintessence Int, 37:297-303, 2006.
  29. Moura JS, Lima EM, Paes Leme AF, et al. : Effect of luting cement on dental biofilm composition and secondary caries around metallic restorations in situ. Oper Dent, 29:509-514, 2004.
  30. Shinkai RS, Cury AA, Cury JA : In vitro evaluation of secondary caries development in enamel and root dentin around luted metallic restoration. Oper Dent, 26:52-59, 2001.
  31. Gonzalez Ede H, Yap AU, Hsu SC : Demineralization inhibition of direct tooth-colored restorative materials. Oper Dent, 29:578-585, 2004.
  32. Han L, Edward C, Okamoto A, et al. : A comparative study of fluoride-releasing adhesive resin materials. Dent Mater J, 21:9-19, 2002. https://doi.org/10.4012/dmj.21.9
  33. Yamamoto H, Iwami Y, Unezaki T, et al. : Fluoride uptake around cavity walls; two-dimensional mapping by electron probe microanalysis. Oper Dent, 25:104-112, 2000.
  34. Retief DH, Bradley EL, Denton JC, Switzer P : Enamel and cementum fluoride uptake from a glass ionomer cement. Caries Res, 18:250-257, 1984. https://doi.org/10.1159/000260773
  35. Silverstone LM : Effect of oral fluid and synthetic calcifying fluids in vitro on remineralization of enamel lesion. Clin Prev Dent, 4:13-22, 1982.
  36. Hicks MJ, Flaitz CM, Silverstone LM : Initiation and progression of caries-like lesions of enamel: effect of periodic treatment with synthetic saliva and sodium fluoride. Caries Res, 19:481-489, 1985. https://doi.org/10.1159/000260886
  37. Verbeeck RM, De Maeyer EA, Marks LA, et al. : Fluoride release process of (resin-modified) glassionomer cements versus (polyacid-modified) composite resins. Biomaterials, 19:509-519, 1998. https://doi.org/10.1016/S0142-9612(97)00131-2
  38. Attin T, Kielbassa AM, Plogmann S, Hellwig E. : Fluoride and cations release from compomers in the acidic and neutral environment. Dtsch Zahnarztl Z, 51:675-678, 1996.
  39. Yap AU, Tham SY, Zhu LY, Lee HK : Short-term fluoride release from various aesthetic restorative materials. Oper Dent, 27:259-265, 2002.
  40. Heinrich-Weltzien R, K?hnisch J, Ifland S, et al. : Detection of initial caries lesions on smooth surfaces by quantitative light-induced fluorescence and visual examination: an in vivo comparison. Eur J Oral Sci, 113:494-498, 2005. https://doi.org/10.1111/j.1600-0722.2005.00255.x
  41. Ando M, van Der Veen MH, Schemehorn BR, Stookey GK : Comparative study to quantify demineralized enamel in deciduous and permanent teeth using laser- and light-induced fluorescence techniques. Caries Res, 35:464-470, 2001. https://doi.org/10.1159/000047491
  42. Al-Khateeb S, Forsberg CM, de Josselin de Jong E, Angmar-Mansson B : A longitudinal laser fluorescence study of white spot lesions in orthodontic patients. Am J Orthod Dentofacial Orthop, 113:595-602, 1998. https://doi.org/10.1016/S0889-5406(98)70218-5
  43. Dunne SM, Goolnik JS, Millar BJ, Seddon RP : Caries inhibition by a resin-modified and conventional glass ionomer cement, in vitro. J Dent, 24:91-94, 1996. https://doi.org/10.1016/0300-5712(95)00051-8
  44. McCabe JF : Resin-modified glass-ionomers. Biomaterials, 19:521-527, 1998. https://doi.org/10.1016/S0142-9612(98)00132-X
  45. Atkinson HF, Matthews E : An investigation into the permeability of human deciduous enamel. Br Dent J, 86:142-145, 1949.
  46. Takagi S, Liao H, Chow LC : Effect of tooth-bound fluoride on enamel demineralization/ remineralization in vitro. Caries Res, 34:281-288, 2000. https://doi.org/10.1159/000016603
  47. 송주현, 김영재, 김정욱 등 : QLF를 이용한 항우식 제품의 인공우식 재광화 효과에 대한 연구. 대한소아치과학회지, 35: 287-295, 2008.