한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권1호
  • /
  • Pages.62-65
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  • 2010
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

Arowana(Scleropages formosus)에서 Hemolysin Gene을 지닌 Aeromonas sobria 분리 및 특성

Isolation of Aeromonas sobria Containing Hemolysin Gene from Arowana (Scleropages formosus)

  • 전진우 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 김지형 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 카시아노 허모피아 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 데니스 고메즈 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 신상필 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 한지은 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단) ;
  • 박세창 (서울대학교 수의과대학 수생동물질병학연구실, BK21 수의과학 (연구인력양성) 사업단)
  • Jun, Jin-Woo (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Ji-Hyung (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Casiano, Choresca Jr. (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Dennis, K. Gomez (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Shin, Sang-Phil (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Han, Jee-Eun (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University) ;
  • Park, Se-Chang (Laboratory of Aquatic Animal Medicine and BK 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University)
  • 발행 : 2010.02.28
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초록

Arowana (Scleropages formosus) is the most valuable group of ornamental fishes and very much in demand in the ornamental fish trade and commands high price ranging from hundreds to thousands of dollars per fish. In this paper, we described a case of mortality of arowana from a private aquarium in Korea. A bacterial pathogen from fish organs (brain, kidney, liver) was cultured, identified and confirmed using Vitek System 2, API 20E test, multiplex PCR and 16S rRNA gene sequencing. The morphological and biochemical properties of the bacterium isolated from the brain, kidney and liver of the fish were similar to Aeromonas sobria. Positive amplification products using the multiplex PCR assay for detection of A. sobria were obtained from these organs. The 16S rRNA gene of the isolates from fish was identical and exhibited 100% sequence similarity with A. sobria (AY987762.1) strain available from GenBank. This bacterium contained hemolysin gene, a virulence factor that plays an important role in outbreaks of disease and is pathogenic to humans as well as in fish. Although this opportunistic bacterium was isolated from a fish without any external symptoms, this pathogen may act as a reservoir and enhance chances of zoonosis to human such as during handling.

키워드

참고문헌

  1. Alfred ER. The fresh-water food fishes of Malaya. Scleropages formosus (Muller and Schlegel). Fed Malay St Mus J 1964; 9: 80-83.
  2. Austin B, Altwegg M, Gosling PJ, Joseph SW. Fish pathogens. In: The genus Aeromonas. San Francisco: John Wiley & Sons Co. 1996: 198-281.
  3. Bauer AW, Kirby WMM, Sherris JC, Truck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45: 493-496.
  4. Borrell N, Figueras MJ, Guarro J. Phenotypic identification of Aeromonas genomospecies from clinical and environmental sources. Can J Microbiol 1998; 40: 103-108.
  5. Dawes J, Lim LL, Cheong L. CITES and the Dragon Fish. In: The Dragon Fish. England: Kingdom Books. 1999: 34-41.
  6. Fernando AA, Lim LC, Jeyaseelan K, Teng SW, Liang MC, Yeo CK. DNA fingerprinting: application to conservation of the CITES-listed dragon fish; Scleropages formosus (Osteoglossidae). Aquar Sci Conserv 1997; 1: 91-104. https://doi.org/10.1023/A:1018387601905
  7. Wang G, Clark CG, Liu C, Pucknell C, Munro CK, Kruk TMAC, Caldeira R, Woodward DL, Rodgers FG. Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. J Clin Microbiol 2003; 41: 1048-1054. https://doi.org/10.1128/JCM.41.3.1048-1054.2003
  8. Greenwood PH, Rosen DE, Weitzman SH, Myers GS. Phyletic studies of teleostean fishes, with a provisional classification of living form. Bull Amer Mus Nat Hist 1996; 131: 338-456.
  9. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Facultatively anaerobic gram-negative rods. In: Bergey's manual of determinative bacteriology, 9th ed. Baltimore: Williams and Wilkins Co. 1994: 177-204.
  10. Janda JM, Abbott SL. Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations, and unanswered questions. Clin Infect Dis 1998; 27: 332-344. https://doi.org/10.1086/514652
  11. Joseph J, Evans D, Broad S. International trade in Asian Bonytongues. Traffic Bull 1983; 7: 73-76.
  12. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. Color atlas and textbook of diagnostic microbiology, 3rd ed. Philadelphia: J.B. Lippincott Co. 1988: 489-491.
  13. Kottelat M, Whitten AJ. Freshwater biodiversity in Asia with special reference to fish. Washington DC: World Bank- Technical Papers. 1996: 58-59.
  14. Ng PKL, Tan HH. Freshwater fishes of Southeast Asia: potential for the aquarium fish trade and conservation issues. Aquar Sci Conserv 1997; 1: 79-90. https://doi.org/10.1023/A:1018335617835
  15. Pemberton JM, Kidd SP, Schmidt R. Secreted enzymes of Aeromonas. FEMS Microbiol Lett 1997; 152: 1-10. https://doi.org/10.1111/j.1574-6968.1997.tb10401.x
  16. Santos JA, Gonzalez CJ, Otero A, Garcia-Lopez ML. Hemolytic activity and siderophore production in different Aeromonas species isolated from fish. Appl Environ Microbiol 1999; 65: 5612-5614.
  17. Soler L, Figueras MJ, Chacon MR, Vila J, Marco F, Martinez- Murcia AJ, Guarro J. Potential virulence and antimicrobial susceptibility of Aeromonas popoffii recovered from freshwater and seawater. FEMS Immunol Med Microbiol 2002; 32: 243-247. https://doi.org/10.1111/j.1574-695X.2002.tb00560.x
  18. Kita-Tsukamoto K, Oyaizu H, Nanba K, Simidu U. Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int J Syst Bacteriol 1993; 43: 8-19. https://doi.org/10.1099/00207713-43-1-8
  19. Wahli T, Burr SE, Pugovkin D, Mueller O, Frey J. Aeromonas sobria, a causative agent of disease in farmed perch, Perca fluviatilis L. J Fish Dis 2005; 28: 141-150. https://doi.org/10.1111/j.1365-2761.2005.00608.x