한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권1호
  • /
  • Pages.46-49
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  • 2010
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

제주말에서 난포 크기에 따른 난포란의 체외성숙

Effect of Follicle Size in Jeju Mare on Oocytes Matured in vitro

  • 류재규 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 강태영 (제주대학교 수의과대학)
  • Yoo, Jae-Gyu (Division of Animal Biotechnology, National Institute of Animal Science, RDA) ;
  • Kang, Tae-Young (College of Veterinary Medicine, Jeju National University)
  • 발행 : 2010.02.28
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초록

In this study, we investigated the number of follicles, oocyte recovery rate and oocyte competence after in vitro maturation according to the size of follicle. And equine oocyte competence after in vitro maturation was investigated in terms of the diameter of follicle with criteria of maturation: nuclear stage after Hoechst staining. The average number of follicles per ovary with middle size (11-20 mm, 2.68) was higher than those of small (5-10 mm, 0.74) and large size follicle (> 21 mm, 1.63), therefore medium follicle (53.1%) had higher proportion than other size of follicles. The average numbers of follicle per ovary was 5.05. The rate of oocyte recovery in small (54.5%) and middle follicle (50%) was higher than that in large follicle (40.9%). After culture for 48 h in Medium 199, 50%, 45.5%, and 44.4% of oocytes from the follicles with diameters of 5-10, 11-20, > 21 mm, respectively reached the metaphase II stage. This is the first report showing number of follicle, oocyte recovery rate according to follicular size, and in vitro oocyte maturation in Jeju mare in Korea. To fulfill in vitro equine embryo production, further studies such as the seasonal effect, in vitro fertilization etc is need.

키워드

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