The Korean Journal of Physiology and Pharmacology

  • 제14권1호
  • /
  • Pages.51-57
  • /
  • 2010
  • /
  • 1226-4512(pISSN)
  • /
  • 2093-3827(eISSN)
대한약리학회 (The Korean Society of Pharmacology)
권호 표지
DOI QR코드

DOI QR Code

Calcium Sensitization Induced by Sodium Fluoride in Permeabilized Rat Mesenteric Arteries

  • Yang, En-Yue (Department of Pharmacology, Kyungpook National University School of Medicine) ;
  • Cho, Joon-Yong (Department of Thoracic and Cardiovascular Surgery, Kyungpook National University School of Medicine) ;
  • Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Kim, In-Kyeom (Department of Pharmacology, Kyungpook National University School of Medicine)
  • 발행 : 2010.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

It was hypothesized that NaF induces calcium sensitization in $Ca^{2+}$-controlled solution in permeabilized rat mesenteric arteries. Rat mesenteric arteries were permeabilized with $\beta$-escin and subjected to tension measurement. NaF potentiated the concentration-response curves to $Ca^{2+}$ (decreased $EC_{50}$ and increased $E_{max}$). Cumulative addition of NaF (4.0, 8.0 and 16 mM) also increased vascular tension in $Ca^{2+}$-controlled solution at pCa 7.0 or pCa 6.5, but not at pCa 8.0. NaF-induced vasocontraction and $GTP{\gamma}S$-induced vasocontraction were not additive. NaF-induced vasocontraction at pCa 7.0 was inhibited by pretreatment with Rho kinase inhibitors H1152 or Y27632 but not with a MLCK inhibitor ML-7 or a PKC inhibitor Ro31-8220. NaF induces calcium sensitization in a $Ca^{2+}$ dependent manner in $\beta$-escin-permeabilized rat mesenteric arteries. These results suggest that NaF is an activator of the Rho kinase signaling pathway during vascular contraction.

키워드

참고문헌

  1. Hirano K. Current topics in the regulatory mechanism underlying the $Ca^{2+}$ sensitization of the contractile apparatus in vascular smooth muscle. J Pharmacol Sci. 2007;104:109-115. https://doi.org/10.1254/jphs.CP0070027
  2. Somlyo AP, Somlyo AV. $Ca^{2+}$ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase. Physiol Rev. 2003;83:1325-1358. https://doi.org/10.1152/physrev.00023.2003
  3. Budzyn K, Marley PD, Sobey CG. Targeting Rho and Rho-kinase in the treatment of cardiovascular disease. Trends Pharmacol Sci. 2006;27:97-104. https://doi.org/10.1016/j.tips.2005.12.002
  4. Murthy KS, Makhlouf GM. Fluoride activates G protein-dependent and -independent pathways in dispersed intestinal smooth muscle cells. Biochem Biophys Res Commun. 1994;202:1681-1687. https://doi.org/10.1006/bbrc.1994.2128
  5. Bogatcheva NV, Wang P, Birukova AA, Verin AD, Garcia JG. Mechanism of fluoride-induced MAP kinase activation in pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2006;290:L1139-1145. https://doi.org/10.1152/ajplung.00161.2005
  6. Fujita A, Takeuchi T, Nakajima H, Nishio H, Hata F. Involvement of heterotrimeric GTP-binding protein and rho protein, but not protein kinase C, in agonist-induced $Ca^{2+}$ sensitization of skinned muscle of guinea pig vas deferens. J Pharmacol Exp Ther. 1995;274:555-561.
  7. Yoshimura H, Jones KA, Perkins WJ, Kai T, Warner DO. Calcium sensitization produced by G protein activation in airway smooth muscle. Am J Physiol Lung Cell Mol Physiol. 2001;281:L631-638. https://doi.org/10.1152/ajplung.2001.281.3.L631
  8. Jeon SB, Jin F, Kim JI, Kim SH, Suk K, Chae SC, Jun JE, Park WH, Kim IK. A role for Rho kinase in vascular contraction evoked by sodium fluoride. Biochem Biophys Res Commun. 2006;343:27-33. https://doi.org/10.1016/j.bbrc.2006.02.120
  9. Yang E, Jeon SB, Baek I, Chen ZA, Jin Z, Kim IK. 17beta-estradiol attenuates vascular contraction through inhibition of RhoA/Rho kinase pathway. Naunyn Schmiedebergs Arch Pharmacol. 2009;380:35-44. https://doi.org/10.1007/s00210-009-0408-x
  10. Todoroki-Ikeda N, Mizukami Y, Mogami K, Kusuda T, Yamamoto K, Miyake T, Sato M, Suzuki S, Yamagata H, Hokazono Y, Kobayashi S. Sphingosylphosphorylcholine induces $Ca^{2+}$-sensitization of vascular smooth muscle contraction: possible involvement of rho-kinase. FEBS Lett. 2000;482:85-90. https://doi.org/10.1016/S0014-5793(00)02046-9
  11. Ryu SK, Ahn DS, Cho YE, Choi SK, Kim YH, Morgan KG, Lee YH. Augmented sphingosylphosphorylcholine-induced $Ca^{2+}$-sensitization of mesenteric artery contraction in spontaneously hypertensive rat. Naunyn Schmiedebergs Arch Pharmacol. 2006;373:30-36. https://doi.org/10.1007/s00210-006-0036-7
  12. Choi SK, Ahn DS, Lee YH. Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery. Cardiovasc Res. 2009;82:324-332.
  13. Durlu-Kandilci NT, Brading AF. Involvement of Rho kinase and protein kinase C in carbachol-induced calcium sensitization in beta-escin skinned rat and guinea-pig bladders. Br J Pharmacol. 2006;148:376-384.
  14. Niiro N, Koga Y, Ikebe M. Agonist-induced changes in the phosphorylation of the myosin- binding subunit of myosin light chain phosphatase and CPI17, two regulatory factors of myosin light chain phosphatase, in smooth muscle. Biochem J. 2003;369:117-128. https://doi.org/10.1042/BJ20021040
  15. Chiba Y, Takeyama H, Sakai H, Misawa M. Effects of Y-27632 on acetylcholine-induced contraction of intact and permeabilized intrapulmonary bronchial smooth muscles in rats. Eur J Pharmacol. 2001;427:77-82. https://doi.org/10.1016/S0014-2999(01)01225-0
  16. Johnson RP, El-Yazbi AF, Takeya K, Walsh EJ, Walsh MP, Cole WC. $Ca^{2+}$ sensitization via phosphorylation of myosin phosphatase targeting subunit at threonine-855 by Rho kinase contributes to the arterial myogenic response. J Physiol. 2009;587:2537-2553. https://doi.org/10.1113/jphysiol.2008.168252
  17. Otto B, Steusloff A, Just I, Aktories K, Pfitzer G. Role of Rho proteins in carbachol-induced contractions in intact and per-meabilized guinea-pig intestinal smooth muscle. J Physiol. 1996;496:317-329. https://doi.org/10.1113/jphysiol.1996.sp021687
  18. Gong MC, Iizuka K, Nixon G, Browne JP, Hall A, Eccleston JF, Sugai M, Kobayashi S, Somlyo AV, Somlyo AP. Role of guanine nucleotide-binding proteins--ras-family or trimeric proteins or both--in $Ca^{2+}$ sensitization of smooth muscle. Proc Natl Acad Sci U S A. 1996;93:1340-1345 https://doi.org/10.1073/pnas.93.3.1340
  19. Wilson DP, Susnjar M, Kiss E, Sutherland C, Walsh MP. Thromboxane A2-induced contraction of rat caudal arterial smooth muscle involves activation of $Ca^{2+}$ entry and $Ca^{2+}$ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697. Biochem J. 2005;389:763-774. https://doi.org/10.1042/BJ20050237
  20. Kitazawa T, Takizawa N, Ikebe M, Eto M. Reconstitution of protein kinase C-induced contractile $Ca^{2+}$ sensitization in triton X-100-demembranated rabbit arterial smooth muscle. J Physiol. 1999;520:139-152. https://doi.org/10.1111/j.1469-7793.1999.00139.x
  21. Kobayashi S, Somlyo AP, Somlyo AV. Guanine nucleotide- and inositol 1,4,5-trisphosphate-induced calcium release in rabbit main pulmonary artery. J Physiol. 1988;403:601-619. https://doi.org/10.1113/jphysiol.1988.sp017267
  22. Antonny B, Sukumar M, Bigay J, Chabre M, Higashijima T. The mechanism of aluminum-independent G-protein activation by fluoride and magnesium. 31P NMR spectroscopy and fluorescence kinetic studies. J Biol Chem. 1993;268:2393-2402.
  23. Blackmore PF, Exton JH. Studies on the hepatic calcium-mobilizing activity of aluminum fluoride and glucagon. Modulation by cAMP and phorbol myristate acetate. J Biol Chem. 1986;261:11056-11063.
  24. Cockcroft S, Taylor JA. Fluoroaluminates mimic guanosine 5'-[gamma-thio]triphosphate in activating the polyphosphoinositide phosphodiesterase of hepatocyte membranes. Role for the guanine nucleotide regulatory protein Gp in signal transduction. Biochem J. 1987;241:409-414. https://doi.org/10.1042/bj2410409
  25. Gilman AG. Guanine nucleotide-binding regulatory proteins and dual control of adenylate cyclase. J Clin Invest. 1984;73:1-4. https://doi.org/10.1172/JCI111179
  26. Kanaho Y, Moss J, Vaughan M. Mechanism of inhibition of transducin GTPase activity by fluoride and aluminum. J Biol Chem. 1985;260:11493-11497.
  27. Bigay J, Deterre P, Pfister C, Chabre M. Fluoroaluminates activate transducin-GDP by mimicking the gamma-phosphate of GTP in its binding site. FEBS Lett. 1985;191:181-185. https://doi.org/10.1016/0014-5793(85)80004-1
  28. Uehata M, Ishizaki T, Satoh H, Ono T, Kawahara T, Morishita T, Tamakawa H, Yamagami K, Inui J, Maekawa M, Narumiya S. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature. 1997;389:990-994. https://doi.org/10.1038/40187
  29. Wirth A, Benyo Z, Lukasova M, Leutgeb B, Wettschureck N, Gorbey S, Orsy P, Horvath B, Maser-Gluth C, Greiner E, Lemmer B, Schutz G, Gutkind JS, Offermanns S. G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension. Nat Med. 2008;14:64-68. https://doi.org/10.1038/nm1666
  30. Lohn M, Plettenburg O, Ivashchenko Y, Kannt A, Hofmeister A, Kadereit D, Schaefer M, Linz W, Kohlmann M, Herbert JM, Janiak P, O'Connor SE, Ruetten H. Pharmacological characterization of SAR407899, a novel rho-kinase inhibitor. Hypertension. 2009;54:676-683. https://doi.org/10.1161/HYPERTENSIONAHA.109.134353

피인용 문헌

  1. Expression of Na+-K+-2Cl− Cotransporter 1 Is Epigenetically Regulated During Postnatal Development of Hypertension vol.24, pp.12, 2010, https://doi.org/10.1038/ajh.2011.136
  2. Upregulation of the Na+-K+-2Cl− cotransporter 1 via histone modification in the aortas of angiotensin II-induced hypertensive rats vol.35, pp.8, 2010, https://doi.org/10.1038/hr.2012.37
  3. 3′,4′-Dihydroxyflavonol reduces vascular contraction through Ca2+ desensitization in permeabilized rat mesenteric artery vol.385, pp.2, 2010, https://doi.org/10.1007/s00210-011-0697-8
  4. Involvement of inhibitor kappa B kinase 2 (IKK2) in the regulation of vascular tone vol.98, pp.10, 2010, https://doi.org/10.1038/s41374-018-0061-4