Effect of Xanthium sibiricum Patr. on Carcinogenesis in Human Cell Lines

창이자의 사람 세포주에서의 발암 억제효과

  • Received : 2010.10.29
  • Accepted : 2010.12.06
  • Published : 2010.12.31

Abstract

Purpose: The aim of this study is to evaluate the effect of Xanthium sibiricum Patr. on carcinogenesis. Method: Water extract from Xanthium sibiricum Patr. (XPW) was prepared and investigated for the potential antitumor activity and inhibition of benzo[a]pyrene-DNA adduct formation and free radical formation. Result: It was shown that the water possess considerable toxicity toward tumor cell lines. Concentration of XPW at 1.0 mg/mL and 2.5 mg/mL resulted in more than 30% inhibition of growth in HeLa cells. Toxicity of XPW to A549 revealed that 54% inhibition of growth at concentration of 2.5 mg/mL. At concentrations of 0.5 mg/mL, 1.0 mg/mL and 2.5 mg/mL of XPW, the binding of [$^3H$]B[a]P metabolites to DNA of human Chang cell was inhibited by 19%, 33%, and 41%, respectively. There 18% and 32% inhibition in the free radical formation with XPW at the concentration of 1.0 mg/mL and 2.5 mg/mL, respectively. Conclusion: Water extract from Xanthium sibiricum Patr. (XPW) has antitumor and cancer chemopreventive activities.

Keywords

References

  1. 이시진 (1992). 본초강목, 북경 인민위생출판. 1102-1104.
  2. Arnould, J. P., Verhoest, P., Bach, V., Libert, J. P., Belegaud, J. (1997). Detection of benzo[a]pyrene-DNA adducts in human placenta and umbilical cord blood. Human Experimental Toxicology, 16, 716-721. https://doi.org/10.1177/096032719701601204
  3. Cerutti, P. A. (1985). Prooxidant states and tumor promotion. Science, 227(4685), 357-381.
  4. Davis, R. W., Thomas, M., Cameron, J., St. John, T. P., Scherer, S., Padgett, R. A., et al. (1980). Rapid DNA isolations for enzymatic and hybridization analysis. Methods in Enzymology, 65, 404-411. https://doi.org/10.1016/S0076-6879(80)65051-4
  5. Kennedy, A. R., Troll, W., Little, J. B. (1984). Role of free radicals in the initiation and promotion of radiation transformation in vitro. Carcinogenesis, 5, 1213-1218. https://doi.org/10.1093/carcin/5.10.1213
  6. Lim, C. K., Yuan, Z. X., Lamb, J. H., White, I. N., De Matteis, F., Smith L. L. et al. (1994). A comparative study of tamoxifen metabolism in female rat, mouse and human liver and human liver microsome. Carcinogenesis, 15, 589-593. https://doi.org/10.1093/carcin/15.4.589
  7. Perera, F. P., Poirier, M. C., Yuspa, S. H., Nakayama, J., Jaretzki, A., Curnen, M. M., Knowies, D. M., Weinstein, I. B., et al. (1982). A pilot project in molecular cancer epidemiology: determination of benzo[a]pyrene-DNA adducts in animal and human tissues by immunoassays. Carcinogenesis (Lond.), 3, 1405-1410. https://doi.org/10.1093/carcin/3.12.1405
  8. Santella, R. M., Yang, X. Y., Hsieh, L. L., Young T. L. (1990). Immunologic methods for the detection of carcinogen adducts in humans. Progress in Clinical Biology Research, 340(C), 247-257.
  9. Sharma, S., Stutzman, J. D., Kelloff, G. J., Steele, V. E. (1994). Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer Research, 54, 5848-5855.
  10. Sims, P., & Grover, P. L. (1974). Epoxides in polycyclic aromatic hydrocarbon metabolism and carcinogenesis. Advanced Cancer Research. 20, 165-274. https://doi.org/10.1016/S0065-230X(08)60111-6
  11. Song, L. L., Kosmeder, J. W., Lee, S. K., Gerhauser, C., Lantvit, D., Moon, R. C., et al. (1999). Cancer chemopreventive activity mediated by 4'-bromoflavone, a potent inducer of phase II detoxification enzymes. Cancer Research, 59, 578-585.
  12. Teel, R. W., Babcock, M. S., Dixit, R., Stoner, G. D. (1986). Ellagic acid toxicity and interaction with benzo[a]pyrene and benzo[a]pyrene 7,8-dihydrodiol in human bronchial epithelial cells. Cell. Biology and Toxicology, 2, 53-62. https://doi.org/10.1007/BF00117707
  13. Troll, W., Klassen, A., Janoff, A. (1970). Tumorigenesis in mouse skin: inhibition by synthetic inhibitors of proteases. Science (Washington DC), 169, 1211-1213. https://doi.org/10.1126/science.169.3951.1211
  14. Tubaro, E., Lotti, B., Croce, C., Caballo, G., Borelli, G. (1980). Liver xanthine oxidase increase in mice in three patholgoical models. A possible defence mechanism. Biochemical Pharmacology, 29, 1939-1943. https://doi.org/10.1016/0006-2952(80)90107-0