Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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The Induction Effect of Apoptosis in A549 Human Lung Cancer Cells by the Trichosanthes Kirilowii Pharmacopuncture Solution

천화분 약침액의 A549 폐암 세포주에서 apoptosis 유발효과

  • Choi, Tae-Yeon (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Sung-Won (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Ryu, Yeon-Hee (Division of Standard Research, Acupuncture, Moxibustion and Meridian Research Center, Korea Institute of Oriental Medicine) ;
  • Ban, Hyo-Jeong (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Seo, Geun-Young (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Jae-Hyo (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Ahn, Seong-Hun (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Sohn, In-Chul (Dept. Of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University)
  • 최태연 (원광대학교 한의과대학 경혈학교실) ;
  • 이성원 (원광대학교 한의과대학 경혈학교실) ;
  • 류연희 (한국한의학연구원) ;
  • 반효정 (원광대학교 한의과대학 경혈학교실) ;
  • 서근영 (원광대학교 한의과대학 경혈학교실) ;
  • 김재효 (원광대학교 한의과대학 경혈학교실) ;
  • 안성훈 (원광대학교 한의과대학 경혈학교실) ;
  • 손인철 (원광대학교 한의과대학 경혈학교실)
  • Received : 2010.11.03
  • Accepted : 2010.12.08
  • Published : 2010.12.27
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Abstract

Objectives : In order to confirm the anti-cancer effect of Trichosanthes kirilowii pharmacopuncture fluid, this study was proceeded. Methods : A549 lung cancer cells were cultured to be treated by Trichosanthes kirilowii pharmacopuncture fluid as dose dependent manner for 72 hours. And then the cell viability, nucleus fragmentaion, p21 and p53 protein expression, Bcl-2 and Bax protein expression, procaspase-3 PARP protein expression. Results : 1. Trichosanthes kirilowii pharmacopuncture fluid decrease A549 cell viability as dose dependent manner. 2. Trichosanthes kirilowii pharmacopuncture fluid induced the nucleus fragmentation in A549 lung cancer cells as dose dependent manner. 3. Trichosanthes kirilowii pharmacopuncture fluid increase the p21 and p53 protein expression. 4. Trichosanthes kirilowii pharmacopuncture fluid decrease the Bcl-2 protein expression but cannot affect the Bax protein expression. 5. Trichosanthes kirilowii pharmacopuncture fluid increase the activation of caspase-3 and PARP protein. Conclusions : As the above results, it was conclused the Trichosanthes kirilowii pharmacopuncture fluid had the anti-cancer effects to induce apoptosis.

Keywords

References

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