Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effects of Various Mulberry Products on the Blood Glucose and Lipid Status of Streptozotocin-induced Diabetic Rats

오디의 가공형태에 따른 Streptozotocin 유발 당뇨쥐의 혈당 및 지질의 상태에 미치는 영향

  • Kim, Hyung-Jin (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Choi, Sang-Won (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Cho, Sung-Hee (Department of Food Science and Nutrition, Catholic University of Daegu)
  • 김형진 (대구가톨릭대학교 식품영양학과) ;
  • 최상원 (대구가톨릭대학교 식품영양학과) ;
  • 조성희 (대구가톨릭대학교 식품영양학과)
  • Received : 2010.10.07
  • Accepted : 2010.12.09
  • Published : 2010.12.31
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Abstract

The study aim was to investigate the effects of three types of mulberry products on the blood glucose and lipid statuses and peroxidative state under diabetic condition. The three types were mulberry liquor prepared by adding 30% ethanol to the crushed fresh fruit, mulberry wine and vinegar by fermentation. For diet experiment the mulberry liquor (M-Liquor), wine (M-Wine), and vinegar (M-Vinegar) were prepared as powders by freeze-drying of the respective product and were added to the diet at the level of 1% and mulberry fruit powder (M-Powder) at the level of 5%. Sprague- Dawley female rats weighing $150{\pm}10\;g$ were randomly assigned to one normal group, and five diabetic groups induced by one intraperitoneal injection of streptozotocin (STZ) at the level of 50 mg/kg. The normal and diabetic control (DM-Control) groups were fed diet without the mulberry products. During twenty-one days of experimental diet, blood glucose was maintained at a low level in the M-Liquor group compared with the DM-Control group. However, serum insulin level was higher in both M-Liquor and M-Vinegar groups after the experimental diet period. Serum levels of total cholesterol and triglyceride (TG) were lower in M-Liquor but HDL-/total cholesterol ratios were higher in the four M groups. The TG liver level was lower in M-Powder and M-Vinegar groups but the cholesterol level was lower in M-Powder than in the DM-Control group. Serum levels of thiobarbituric acid reactive substances were not different among the groups but the liver levels of these substances were lower in the four M groups than in DM-Control. Serum GOT and GPT levels were not changed by the mulberry products. These results indicated that mulberry liquor is the most effective among the four mulberry products for normalizing blood glucose and lipid status and that all the mulberry products were effective in enhancing antioxidant defense in the diabetic state.

본 연구는 오디를 사용하여 제조된 오디침출주, 오디식초의 당뇨조건에서 혈당과 지질 상태 및 체내 과산화상태에 관한 효과를 조사하고자 시도되었다. 오디침출주는 생오디를 분쇄하여 30% 에탄올을 첨가하여 숙성 제조하였으며 오디발효주와 오디식초는 오디추출액에 설탕을 첨가한 후 효모를 이용하여 발효시켜 오디발효주를 제조하고 이 발효주에 초산균을 접종하여 오디식초를 제조하였다. 상기의 생리기능을 조사하고자 시행한 식이실험재료는 제조된 오디 침출주, 발효주 및 식초의 동결건조 분말과 생오디를 동결 건조한 오디분말로 4종류였다. 실험동물로는 Sprague-Dawley 암쥐로 체중이 $150{\pm}10\;g$ 정도 되었을 때 1군의 정상군과 5군의 당뇨균으로 나누고 당뇨군들은 복강으로 streptozotocin (50 mg/kg bw)을 투여하여 당뇨를 유발하였다. 당뇨가 유발된 군들은 당뇨대조군 (DM-Control), 오디분말군 (M-Powder), 오디침출주 (M-Liquor), 오디발효주(M-Wine) 및 오디식초 (M-Vinegar)군으로 다시 나눈 뒤 오디분말군에게는 분말을 식이의 5%, 침출주, 발효주, 식초군에게는 각기 동결건조한 분말을 식이의 1%로 첨가한 식이를 제공하여 24일간 사육하였다. 실험식이 시작 후 21일 동안 추적한 혈당은 오디침출주군이 당뇨대조군에 비하여 지속적으로 낮게 유지하였으며 다른 오디군들은 식이 13일 전후로만 유의하게 낮았다. 그러나 식이 종료 후 측정한 혈청 인슐린은 오디침출주 뿐 아니라 오디식초군에서도 당뇨대조군에 비하여 높았다. 혈청 콜레스테롤과 중성지방도 오디침출주에서 유의하게 낮았으나 HDL-/총콜레스테롤 비율은 모든 오디군에서 증가하였다. 혈청 TBARS 수준은 실험군 간에 차이가 없었으나 간조직 TBARS는 모든 오디군들에서 낮았고 혈청 GOT, GPT, BUN 수준은 오디시료 섭취로 변화가 없었다. 이 결과로서 본 실험에서 사용한 오디제품들은 간 손상을 미치지 않고 오디침출주가 당뇨상태에서 당 및 지질대사 개선 효과가 높다고 생각되며 모든 오디제품은 항산화 효능이 높아 본격적인 제품화가 기대된다.

Keywords

References

  1. Kim SK. Beneficial medicine, mulberry fruit. Bonchohak. Seoul: Younglimsa; 1991. p.598-605
  2. Kim AJ, Kim MW, Woo N, Kim SY, Kim HB, Lim YH, Kim MH. Study on the nutritional composition and antioxidative capacity of mulberry fruit (Ficus-4x). Korean J Food Sci Technol 2004; 36(6): 995-1000
  3. Park SW, Jung YS, Ko KC. Quantitative analysis of anthocyanins among mulberry cultivars and their pharmacological screening. HEB (J Kor Soc Hort Sci) 1997; 38(6): 722-724
  4. Kim HB, Kim SL, Moon JY. Quantification and varietal variation of anthocyanin pigment in mulberry fruits. Korean J Breed 2002; 34(3): 207-211
  5. Kim EO, Yu MW, Suh IH, Lee YJ, Im HH, Kang DH, Choi SW. Comparison of physiochemical properteis of seven different mulberry (Morus alba L.) cultivars. J Korean Soc Food Sci Nutr 2010; 39(10) in press
  6. Kim HB, Kim SL. Quantification and variation of rutin in mulberry fruits. Korean J Seric Sci 2004; 46(1): 1-5
  7. Cha WS, Shin HR, Park JH, Oh SL, Lee WY, Chun SS, Choo JW, Cho YJ. Antioxidant activity of phenol compounds from mulberrry fruits. Korean J Food Preserv 2004; 11(3): 383-387
  8. Lee JY, Moon SO, Kwon YJ, Rhee SJ, Park HR, Choi SW. Identification and quantification of anthocyanins and flavonoids in mulberry (Morus sp.) cultivars. Food Sci Biotechnol 2004; 13 (2): 176-184.
  9. Kwon YJ, Rhee SJ, Chu JW, Choi SW. Comparison of radical scavenging activity of extracts of mulberry juice and cake prepared from mulberry (Morus spp.) fruit. J Food Sci Nutr 2005; 10(2): 111-117 https://doi.org/10.3746/jfn.2005.10.2.111
  10. Kim SY, Park KJ, Lee WC. Antiinflammatory and antioxidative effects of Morus spp fruit extract. Korean J Med Crop Sci 1998; 6(3): 204-209
  11. Kim HJ, Cha JY, Choi ML, Cho YS. Antioxidative activities by water-soluble extracts of Morus alba and Cudrania tricuspidata. J Korean Soc Agric Chem Biotechnol 2000; 43(2): 148-152
  12. Shin YW, Lee SK, Kwon YJ, Rhee SJ, Choi SW. Radical scavenging activity of phenolic compounds from mulberry (Morus spp.) cake. J Food Sci Nutr 2005; 10(4): 326-332 https://doi.org/10.3746/jfn.2005.10.4.326
  13. Kwon EH, Jung MA, Rhee SJ, Choi SW, Cho SH. Antioxidant effects and improvement of lipid metabolism of mulberry fruit, mulberry leaves and silkworm powder with different mixing ratios in streptozotocin-induced diabetic rats. Korean J Nutr 2006; 39(2): 91-99
  14. Kwon EH, Jang HS, Kim SW, Choi SW, Rhee SJ, Cho SH. Effects of mulberry juice and cake powder on blood glucose and lipid lowering and erythrocyte antioxidative enzyme activities in streptozotocin-induced diabetic rats. Korean J Nutr 2007; 40(3): 199-210
  15. Kim HB, Kim SY, Lee HY, Kim SL, Kang SW. Protective effect against neuronal cell and inhibitory activity against bacteria of mulberry fruit extracts. Korean J Crop Sci 2005; 50(S): 220-223
  16. Kim AJ, Kim HB, Bang IS, Kim SY. The effects of mulberry fruit extract supplementation on the serum mineral contents and oxidative stress markers of middle-aged humans living in Choongnam area. Korean J Food Sci Technol 2006; 38(2): 284-289
  17. Kim TY, Kwon YB, Lee JH, Yang IS, Youm JK, Lee HS, Moon JY. A study on the antidiabetic effect of mulberry fruits. Korean J Seri Sci 1996; 38(2): 100-107
  18. Kim HB, Kim SY, Ryu KS, Lee WC, Moon JY. Effect of methnol extract from mulberry fruit on the lipid metabolism and liver function in cholesterol-induced hyperlipedema rats. Korean J Seri Sci 2001; 43(2): 104-108
  19. Kim AJ, Park SJ, Rho JO. Mulberry fruit extract consumption is inversely associated with hyperlipidemia in middle-aged men. Korean J Food & Nutr 2008; 21(2): 121-126
  20. Lee SH, Kim HG. Effect of mulberry extracts on secretion of MMPs and plasmin in U-373-MG cells. Korean J Biotechnol Bioeng 2008; 23(2): 142-146
  21. Kim IS, Lee JY, Rhee SJ, Yun KS, Choi SW. Preparation of minimally processed mulberry (Morus spp.) juices. Korean J Food Sci Technol 2004; 36(2): 321-328
  22. Rommel A, Heatherbell DA, Wrolstad RE. Red raspberry juice and wine: Effects of processing and storage on anthocyanin pigment composition, color and appearance. J Food Sci 1990; 55(4): 1011-1017 https://doi.org/10.1111/j.1365-2621.1990.tb01586.x
  23. Jung GT, Ju IO, Choi DG. Quality characteristics and manufacture of mulberry wine. Korean J Food Preserv 2005; 12(1): 90-94
  24. Kim YS, Jeong DY, Shin DH. Optimum fermentation conditions and fermentation characteristics of mulberry (Morus alba) wine. Korean J Food Sci Technol 2008; 40(1): 1-7
  25. Jung J, Son MY, Jung S, Nam P, Sung JS, Lee SJ, Lee KG. Antioxidant properties of Korean black raspberry wines and their apoptotic effects on cancer cells. J Sci Food Agric 2009; 89(6): 970-977 https://doi.org/10.1002/jsfa.3540
  26. Minussi RC, Rossi M, Bologna L, Cordi L, Rotilio D, Pastore GM, Duran N. Phenolic compounds and total antioxidant potential of commercial wines. Food Chem 2003; 82(3): 409-416 https://doi.org/10.1016/S0308-8146(02)00590-3
  27. Cho MZ, Kim AJ. The Manufacturing and physiological evaluation of mulberry fruit tea. Korean J Food & Nutr 2007; 20(2): 173-178
  28. Kang YS, Cho TO, Hong JS. Quality Characteristics of Jeolpyon with added mulberry fruit powder. Korean J Food Cookery Sci 2009; 25(4): 513-519
  29. Kim AJ, Kim MW, U NLY, Kim MH, Im YH. Quality characteristics of Oddi-Pyun prepared with various levels of mulberry fruit extract. Korean J Food Cookery Sci 2003; 19(6): 708-714
  30. Lee JH, Woo KJ, Choi WS, Kim AJ, Kim MW. Quality characteristics of starch Oddi Dasik added with mulberry fruit juice. Korean J Food Cookery Sci 2005; 21(6): 629-636
  31. Lee YJ, Sim CH, Chun SS. Physical and sensory properties of chiffon cake prepared with mulberry powder. Korean J Food & Nutr 2009; 22(4): 508-516
  32. Kim HB, Kim AJ, Yuh CS, Chang SJ. Sensory Characteristics and nutritional analysis of sherbet ice-cream with mulberry fruit. Korean J Seric Sci 2003; 45(2): 85-89
  33. Choi SW, Cho SH, Seo IH, Kim EO, Yu MH, Park EJ, Kim HJ. Development of value-added processed products using mulberry (Morus alba L.) fruit. Final Report of Technology Development Project for Agriculture and Fisheries, Gyeongsangbukdo, Republic of Korea; 2010. 5. 7, p.20
  34. Tepper BJ, Kanarek RB. Dietary self-selection patterns of rats with mild diabetes. J Nutr 1985; 115(6): 699-709
  35. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc committee on the reformation of the AIN-76A rodent diet. J Nutr 1993; 123(11): 1939-1951
  36. Omodeo Sale F, Marchesini S, Fishman PH, Berra B. A sensitive enzymatic assay for determination of cholesterol in lipid extracts. Anal Biochem 1984; 142(2): 347-350 https://doi.org/10.1016/0003-2697(84)90475-5
  37. Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 1976; 15(2): 212-216 https://doi.org/10.1016/0006-2944(76)90049-1
  38. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  39. Hong JH, Kim SW, Choi KH, Choi SW, Rhee SJ. Inhibitory effects of mulberry fruit on intestinal disaccharidase activity and hyperglycemia in streptozotocin-induced diabetic rats. Nutr Sci 2004; 7(4): 201-207
  40. Cho SH, Lee HR, Kim TH, Choi SW, Lee WJ, Choi Y. Effects of defatted safflower seed extract and phenolic compounds in diet on plasma and liver lipid in ovariectomized rats fed high cholesterol diets. J Nutr Sci Vitaminol 2004;50(1): 32-37 https://doi.org/10.3177/jnsv.50.32
  41. Cho SH, Rhee SJ, Choi SW, Choi Y. Effects of forsythia fruit extracts and lignan on lipid metabolism. Biofactors 2004; 22(1- 4): 161-163 https://doi.org/10.1002/biof.5520220132
  42. Cho SH, Choi SW, Lee HR, Lee JY, Lee WJ, Choi Y. Safety and effects on lipid parameters of Rubus coreanus and Atractylodes japonica on ovariectomized rats. J Food Sci Nutr 2004; 9(4): 361-366 https://doi.org/10.3746/jfn.2004.9.4.361
  43. Jang YH, Choi SW, Cho SH. Effect of Eisenia bicyclis and its pill on serum lipid status in rats fed high fat diet. Korean J Nutr 2008; 41(1): 5-12
  44. Kang BT, Yoon OH, Lee JW, Kim SH. Qualitative properties of wild grape wine having different aging periods. Korean J Food & Nutr 2009; 22(4): 548-553
  45. Choi HS, Kim MK, Park HS, Shin DH. Changes in physicochemical characteristics of Bokbunja (Rubus coreanus Miq.) wine during fermentation. Korean J Food Sci Technol 2005; 37(4): 574-578
  46. Lee SM, Choi Y, Kim Y, Kim DJ, Lee J. Antioxidant activity of vinegars commercially available in Korean markets. Food Engineering Progress 2009; 13(3): 221-225
  47. Hwang YC, Shin KS. Characterization of immuno-stimulating polysaccharides isolated from Korean persimmon vinegar. Korean J Food Sci Technol 2008; 40(2): 220-227

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