Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Gastrodia elata Blune Water Extract on RANKL-induced Osteoclast Differentiation

천마가 RANKL에 의해 유도된 파골세포의 분화에 미치는 효과

  • Choi, Yun-Hong (Department of Anatomy & Institute for Environmental Science, Wonkwang University) ;
  • Song, Jeong-Hoon (Department of Plastic & Reconstructive Surgery, Wonkwang University) ;
  • Jang, Sung-Jo (Department of Neurosurgery, School of Medicine, Wonkwang University) ;
  • Kim, Jin-Kook (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Choi, Min-Kyu (Department of Anatomy & Institute for Environmental Science, Wonkwang University)
  • 최윤홍 (원광대학교 의과대학 해부학교실 및 환경과학연구소) ;
  • 송정훈 (원광대학교 의과대학 성형외과학교실) ;
  • 장성조 (원광대학교 의과대학 신경외과학교실) ;
  • 김진국 (원광대학교 한의과대학 병리학교실) ;
  • 최민규 (원광대학교 의과대학 해부학교실 및 환경과학연구소)
  • Received : 2010.09.14
  • Accepted : 2010.10.15
  • Published : 2010.10.25
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Abstract

Impairment of balance between bone-resorbing osteoclasts and bone-forming osteoblasts result in bone disease. Especially, increased osteoclast formation and activity are responsible for bone diseases such as osteoporosis, rheumatoid arthritis, periodontal disease. Natural metabolites of plants have recently received much attention as an alternative tools for the development of novel therapeutic strategy. The aim of this study was to search the natural products to inhibit osteoclast differentiation and was to evaluate of its mechanism. Water extract of Gastrodia elata Blune significantly inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation in bone marrow macrophages (BMMs) in a dose dependent manner. However, water extract of Gastrodia elata Blune did not affect cytotoxicity when compared with control. The mRNA expression of c-Fos, NFATc1, and TRAP induced by RANKL was inhibited by water extract of Gastrodia elata Blune treatment. Also, water extract of Gastrodia elata Blune inhibited the protein expression of c-Fos and NFATc1 expression in BMMs treated with RANKL. Water extract of Gastrodia elata Blune suppressed the phosphorylation of p38 induced by RANKL. In general, RANKL considerably inhibited the expression level of Id2 and MafB known as negative regulators of osteoclastogenesis, but RANKL did not inhibit Id2 and MafB expression in BMMs when it was co-treated with Gastrodia elata Blune. Taken together, these results suggest that Gastrodia elata Blune may be a useful drug in the treatment of bone-related disease.

Keywords

References

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