Journal of Korean Neurosurgical Society

  • 제47권1호
  • /
  • Pages.7-10
  • /
  • 2010
  • /
  • 2005-3711(pISSN)
  • /
  • 1598-7876(eISSN)
대한신경외과학회 (The Korean Neurosurgical Society)
권호 표지
DOI QR코드

DOI QR Code

Intracranial Pressure and Experimental Model of Diffuse Brain Injury in Rats

  • Blaha, Martin (Department of Neurological Surgery, University Hospital Motol) ;
  • Schwab, Juraj (Department of Neurological Surgery, University Hospital Motol) ;
  • Vajnerova, Olga (Departments of Physiology, Second Faculty of Medicine Charles University) ;
  • Bednar, Michal (Department of Anaesthesiology, Clinicum) ;
  • Vajner, Ludek (Histology and Embryology, Second Faculty of Medicine Charles University) ;
  • Michal, Tichy (Departments of Physiology, Second Faculty of Medicine Charles University)
  • 발행 : 2010.01.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: In this study, we present a simple closed head injury model as a two-stage experiment. The height of the weight drop enables gradation of head trauma severity. Methods: The head injury device consists of three parts and there are three adjustable parameters-weight (100-600 g). height of fall (5-100 cm) and elasticity of the springs. Thirty male Wistar rats underwent monitoring of intracranial pressure with and without induction of the head injury. Results: The weight drop from 45 to 100 cm led to immediate seizure activity and early death of the experimental animals. Severe head injury was induced from 40 cm weight drop. There was 50% mortality and all surviving rats had behavioral deterioration. Intracranial pressure was 9.3${\pm}$3.76 mmHg. Moderate head injury was induced from 35 cm, mortality decreased to 20-40%, only half of the animals showed behavioral pathology and intracranial pressure was 7.6${\pm}$3.54 mmHg. Weight drop from 30 cm caused mild head injury without mortality and neurological deterioration. Intracranial pressure was slightly higher compared to sham group- 5.5${\pm}$0.74 mmHg and 2.9${\pm}$0.81 mmHg respectively. Conclusion: This model is an eligible tool to create graded brain injury with stepwise intracranial pressure elevation.

키워드

참고문헌

  1. Cesak T, Hobza V : Epidemiologie mozkovych traumat in Smrcka M(ed) : Poraneni mozku, ed 1. Prague : Grada Publishing, 2001, pp19-28
  2. Clausen F, Hillered L : Intracranial pressure changes during fluid percussion, controlled cortical impact and weight drop injury in rats. Acta Neurochir (Wien) 147 : 775-780; discussion 780, 2005 https://doi.org/10.1007/s00701-005-0550-2
  3. Dixon CE, Lyeth BG, Povlishock JT, Findling RL, Hamm RJ, Marmarou A, et al. : A fluid percussion model of experimental brain injury in the rat. J Neurosurg 67 : 110-119, 1987 https://doi.org/10.3171/jns.1987.67.1.0110
  4. Engelborghs K, Verlooy J, Van Reempts J, van Deuren B, van de Ven M, Borgers M : Temporal changes in intracranial pressure in a modified experimental model of closed head injury. J Neurosurg 89 : 796-806, 1988
  5. James HE, Schneider S : Cryogenic brain oedema : loss of cerebrovascular autoregulation as a cause of intracranial hypertension. Implications for treatment. Acta Neurochir Suppl (Wien) : 5179-5181, 1990
  6. Jeon IC, Kim OL, Kim MS, Kim SH, Chang CH, Bai DS : The effect of premorbid demographic factors on the recovery of neurocognitive function in traumatic brain injury patients. J Korean Neurosurg Soc 44 : 295-302, 2008 https://doi.org/10.3340/jkns.2008.44.5.295
  7. Laurer HL, McIntosh TK : Experimental models of brain trauma. Curr Opin Neurol 12 : 715-721, 1999 https://doi.org/10.1097/00019052-199912000-00010
  8. Lighthall JW : Controlled cortical impact : a new experimental brain injury model. J Neurotrauma 5 : 1-15, 1988 https://doi.org/10.1089/neu.1988.5.1
  9. Marmarou A, Foda MA, van den Brink W, Campbeell J, Kita H, Demetriadou K : A new model of diffuse brain injury in rats. Part I : Pathophysiology and biomechanics. J Neurosurg 80 : 291-300, 1994 https://doi.org/10.3171/jns.1994.80.2.0291
  10. Rooker S, Jorens PG, van Reempts J, Borgers M, Verloy J : Continuous measurement of intracranial pressure in awake rats after experimental closed head injury. J Neurosci Methods 131 : 75-81, 2003 https://doi.org/10.1016/S0165-0270(03)00233-4
  11. Smith DH, Chen XH, Xu BN, McIntosh TK, Gennarelli TA, Meaney DF : Characterization of diffuse axonal pathology and selective hippocampal damage following inertial brain trauma in the pig. J Neuropathol Exp Neurol 56 : 822-834, 1997
  12. Todd MM, Weeks JB, Warner DS : A focal cryogenic brain lesion does not reduce the minimum alveolar concentration for halothane in rats. Anesthesiology 79 : 139-143, 1993 https://doi.org/10.1097/00000542-199307000-00020
  13. Tsai MS, Chou YL, Chang GL, Shen CL : The effect of magnitudes and duration of pressure on cerebral cortex in a rat model. J Clin Neurosci 8 : 157-163, 2001 https://doi.org/10.1054/jocn.2000.0842

피인용 문헌

  1. Experimental traumatic brain injury vol.2, pp.1, 2010, https://doi.org/10.1186/2040-7378-2-16
  2. Epileptogenesis following experimentally induced traumatic brain injury – a systematic review vol.27, pp.3, 2010, https://doi.org/10.1515/revneuro-2015-0050
  3. Effects of Quercetin and Mannitol on Erythropoietin Levels in Rats Following Acute Severe Traumatic Brain Injury vol.60, pp.3, 2017, https://doi.org/10.3340/jkns.2016.0505.015
  4. Elevated intracranial pressure induces IL-1β and IL-18 overproduction via activation of the NLRP3 inflammasome in microglia of ischemic adult rats vol.47, pp.1, 2010, https://doi.org/10.3892/ijmm.2020.4779