Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
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Morphological Characteristics of Medium-Leaf Type Zoysiagrasses (Zoysia spp.) and Their Classification Using RAPDs

중엽형 한국잔디(Zoysia spp.)류의 형태적 특성과 RAPDs를 이용한 분류

  • Choi, Joon-Soo (Department of Green landscape Science, Dankook University)
  • 최준수 (단국대학교 생명자원과학대학 녹지조경학과)
  • Received : 2010.11.15
  • Accepted : 2010.12.10
  • Published : 2010.12.31
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Abstract

Zoysiagrass, especially Jungji implicating medium-leaf type zoysiagrass has been widely used in South Korea recently. This study was carried out to classify 36 selected medium-leaf type zoysiagrass accessions compared to 5 basic zoysiagrass species using RAPDs. Morphological characteristics such as leaf width, leaf angle, leaf sheath length, existance of trichomes and stolon color were measured as useful characteristics for identification of species in Zoysia genus. Nineteen RAPD markers were identified using 8 selected random primers. The dissimilarity coefficient of variants ranged from 0 to 0.736. Three zoysiagrass groups were clustered by dissimilarity coefficient analysis. Group 1 consisted of Z. japonica and some US varieties including 'Zenith' and 'Meyer'. Group 2 consisted of Z. sinica, Z. macrostachya and Korean commercial varieties such as 'Anyang', 'Samdeock', and 'Pyeongdong' medium-leaf type grasses. Group 3 was genetically distinct from Group 1 and Group 2, and included Z. matrella and Z. tenuifolia. 'Anyang', 'Samdeock', and 'Pyeongdong' medium-leaf type zoysiagrasses showed very close genetic relationship with Z. sinica and Z. macrostachya.

한국잔디류중 엽폭이 3~4 mm 정도를 보이는 중지류는 최근에 국내에서 가장 널리 이용되고 있는 잔디이다. 본 연구에서는 한국잔디류 중 중엽형 36 계통과 기본 5종의 근연관계를 확인해 보고자 RAPD 방법을 사용하였다. 형태적 특성조사는 엽폭, 잎각도, 최하위 잎의 잎집 길이, 엽모, 지상포복경의 색 등을 조사하였다. 여덟개의 프라이머를 이용하여 19개의 RAPD 마커를 확인하였다. 마커를 이용하여 유전적 유사도를 조사한 결과 0~0.736의 유전적 변이정도가 확인되었다. RAPD 마커를 이용하여 분류한 결과 3개 군으로 나뉘어 졌다. 제1군에는 들잔디와 함께 미국에서 육성된 'Zenith', 'Meyer'등이 포함되었다. 제2군에는 갯잔디, 왕잔디와 함께 '안양중지', '삼덕중지', '평동중지' 등이 포함되었다. 그리고 제3군은 제1군, 제2군과 비교해 유전적으로 거리가 멀게 나타났으며, 금잔디와 비단잔디가 포함되었다. 한국에서 주로 이용되고 있는 '안양중지', '삼덕중지', 그리고 '평동중지' 류는 갯잔디 및 왕잔디와 유전적 유사도가 높은 것으로 나타났다.

Keywords

References

  1. Choi, J.S. and G.M. Yang. 1996. PCR conditions for effective identification of Korean native zoysiagrass (Zoysia spp.) species by DNA polymorphism. J. Kor. Soc. Hort. Sci. 37(1):166- 170.
  2. Choi, J.S., B.J. Ahn, and G.M. Yang. 1997. Classification of zoysiagrass (Zoysia spp.) native to the southwest coastal regions of Korea using RAPDs. J. Kor. Soc. Hort. Sci. 38(6):789-795.
  3. Choi, J.S. and G.M. Yang. 2006. Sod production in South Korea. Kor. J. Turf. Sci. 20(2):237-251.
  4. Christians, N.E. 2007. Fundamentals of turfgrass management. John Wiley & Sons, Inc. Hoboken, NJ, pp.63-65.
  5. Christians, N.E. and M.C. Engelke. 1994. Choosing the right grass to fit the environment. pp.99-113. In: A.R. Leslie (ed.). Integrated pest management for turf and ornamentals. CRC Press, Levis Publishers.
  6. Diesburg, K.L. 1997. Zoysiagrass breeding in the United States. International symposium of zoysiagrass breeding. Dankook University, Korea. pp.9-14.
  7. Emmons, R.D. 1995. Turfgrass science and management. Delmar Publishers. pp.50-52.
  8. Engelke, M.C., J.J. Murray, and D.Y. Yeam. 1983. Distribution, collection and use of zoysiagrass in the far east, part II. Agronomy abstract p.125.
  9. Fukuoka, H. 1997. Breeding of Zoysia in Japan. International symposium of zoysiagrass breeding. Dankook University, Korea. pp.1-8.
  10. Fukuoka, S., K. Hosaka, and O. Kamijima. 1992. Use of random amplified polymorphic DNAs (RAPDs) for identification of rice accession. Jpn. J. Genet. 67:243-252. https://doi.org/10.1266/jjg.67.243
  11. Mo, S.Y. 1997. Analysis of genetic diversity for the accessions of zoysiagrass and their hybrid lines with morphological characteristics and RAPD markers. MS Thesis, Konkuk University, Korea.
  12. Rogers, S.O. and A.J. Bendich. 1988. Extraction of DNA from plant tissue. Plant molecular biology manual A 6:1-10.
  13. Ronghui, Li. 1993. Lawngrass of China. pp.896-897. In: R.N. Carrow, N.E. Christians, and R.C. Shearman (eds.). 7th International Turfgrass Society Research Journal.
  14. Ruemmele, B.A. and M.C. Engelke. 1990. Zoysiagrass cultivars. Grounds Maintenance. April. pp.92-126.
  15. Turgeon, A.J. 1991. Turfgrass management (3th ed.). Prentice Hall, Upper Saddle River, NJ. pp.77-104.
  16. Wilkinson, J.F. and J.B. Beard. 1972. Electrophoretic identification of Agrostis palustris and Poa pratensis cultivars. Crop Sci. 12:383-384.
  17. Yeam, D.H, J.J. Murray, and G.R. Bauchan. 1987a. Classification of zoysiagrass using morphological traits. J. Kor. Soc. Hort. Sci. Abstract 5(1):128-129.
  18. Yeam, D.H, J.J. Murray, and G.R. Bauchan. 1987b. Classification of zoysiagrass using isozyme traits. J. Kor. Soc. Hort. Sci. Abstract 5(1):130-131.
  19. Yoo, H.K. 1997. Comparison of genetic similarity among species and ecotypes of zoysiagrasses with that among species and cultivars of cool-season turfgrass using RAPD. MS Thesis, Dankook University, Korea.
  20. Yaneshita, M., R. Nagasawa, S. Kaneko, Y. Ogihara, and T. Sasakuma. 1993. Genetic characterization of zoysiagrasses by RFLP analysis of nuclear DNA. pp.786-792. In: R.N. Carrow, N.E. Christians, and R.C. Shearman (eds.). 7th International Turfgrass Society Research