Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Inflammatory Activity of the Total Flavonoid Fraction from Broussonetia papyrifera in Combination with Lonicera japonica

  • Jin, Jeong-Ho (College of Pharmacy, Kangwon National University) ;
  • Lim, Hyun (College of Pharmacy, Kangwon National University) ;
  • Kwon, Soon-Youl (Department Food and Nutrition, Andong National University) ;
  • Son, Kun-Ho (Department Food and Nutrition, Andong National University) ;
  • Kim, Hyun-Pyo (College of Pharmacy, Kangwon National University)
  • Received : 2010.03.12
  • Accepted : 2010.04.09
  • Published : 2010.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

To establish the anti-inflammatory activity of the total flavonoid fraction of the root barks of Broussonetia papyrifera (EBP) and a new formula, the ethanol extract of the root barks of B. papyrifera was fractionated with ethylacetate, yielding the hydrophobic prenylated flavonoid-enriched fraction. EBP and the ethanol extract of the whole Lonicera japonica (ELJ) plant were then mixed at a ratio of 1:1 (w/w) to give a new preparation (BL) in the hope of obtaining an optimal formula with a higher anti-inflammatory activity. Evaluation of the effects of these preparations on A23187-treated rat basophilic leukemia (RBL-1) cells revealed that EBP potently inhibited 5-lipoxygenase (5-LOX), while ELJ showed weak inhibition. Additionally, the mixture (BL) clearly showed stronger inhibitory effects against 5-LOX than either preparation alone. These preparations also inhibited cyclooxygenase-2-catalyzed $PGE_2$ and inducible nitric oxide (NO) synthase-catalyzed NO production by lipopolysaccharide-treated RAW 264.7 cells. When tested against arachidonic acid-induced mouse ear edema, EBP showed strong inhibitory activity at doses of 5-200 mg/kg when administered orally, but BL had obviously stronger inhibitory effects. When tested against ${\lambda}$-carrageenan-induced paw edema in mice, BL showed a potent and synergistic anti-inflammatory effect. In addition, in the acetic acid-induced writhing test, BL was found to have strong analgesic activity at 50-400 mg/kg. Taken together, these results indicate that each of these preparations exert anti-inflammatory activity in vitro and in vivo. In particular, BL showed stronger anti-inflammatory activity than EBP, and these anti-inflammatory effects were partially related to the inhibition of eicosanoid and NO production. BL may be useful for the treatment of human inflammatory disorders.

Keywords

References

  1. Chen, R. M., Hu, L. H., An, T. Y., Li, J. and Shen, Q. (2002). Natural PTP1B inhibitors from Broussonetia papyrifera. Bioorg. Med. Chem. Lett. 12, 3387-3390. https://doi.org/10.1016/S0960-894X(02)00757-6
  2. Cheng, Z. J., Lin, C. N., Hwang, T. L. and Teng, C. M. (2001). Broussochalcone A, a potent antioxidant and effective suppressor of inducible nitric oxide synthase in lipopolysaccharide-activated macrophages. Biochem. Pharmacol. 61, 939-946. https://doi.org/10.1016/S0006-2952(01)00543-3
  3. Chi, Y. S., Cheon, B. S. and Kim, H. P. (2001a). Effect of wogonin, a plant flavone from Scutellaria radix, on the suppression of cyclooxygenase and the induction of inducible nitric oxide synthase in lipolpolysaccharide-treated RAW 264.7 cells. Biochem. Pharmacol. 61, 1195-1203. https://doi.org/10.1016/S0006-2952(01)00597-4
  4. Chi, Y. S., Jong, H. G., Son, K. H., Chang, H. W., Kang, S. S. and Kim, H. P. (2001b). Effects of naturally occurring prenylated flavonoids on arachidonic acid metabolizing enzymes: Cyclooxygenases and lipoxygenases. Biochem. Pharmacol. 62, 1185-1191. https://doi.org/10.1016/S0006-2952(01)00773-0
  5. Damas, J., Bourdon, V., Remacle-Volon, G. and Adam, A. (1990). Kinins and peritoneal exudates induced by carrageenin and zymosan in rats. Br. J. Pharmacol. 101, 418-422. https://doi.org/10.1111/j.1476-5381.1990.tb12724.x
  6. Editorial Committee of Zhong Hua Ben Cao of State Administration of Traditional Chinese Medicine of People’s Republic of China (1999). Zhong Hua Ben Cao vol. 2., pp. 473-474, Shanghai Science and Technology Publishing Co, Shanghai.
  7. Holsapplae, M. P. and Yim, G. K. (1984). Therapeutic reduction of ongoing carrageenin-induced inflammation by lipoxygenase, but not cyclooxygenase inhibitors. Inflammation 8, 223-230. https://doi.org/10.1007/BF00916412
  8. Hwang, J. H. and Lee, B. M. (2007). Inhibitory effects of plant extracts on tyrosinase, L-DOPA oxidation, and melanin synthesis. J. Toxicol. Environ. Health A 70, 393-407. https://doi.org/10.1080/10937400600882871
  9. Inoue, H., Mori, T. and Koshihara, Y. (1988). Sulfidopeptideleukotrienes are major mediators of arachidonic acid-induced mouse ear edema. Prostaglandins 36, 731-739. https://doi.org/10.1016/0090-6980(88)90016-0
  10. Kawai, H., Kutoyanagi, M. and Ueno, A. (1988). Iridoid glucosides from Lonicera japonica thunb. Chem. Pharm. Bull. 36, 3664-3666. https://doi.org/10.1248/cpb.36.3664
  11. Kim, H. K., Namgoong, S. Y. and Kim, H. P. (1993). Anti-inflammatory activity of flavonoids: Mice ear edema inhibition. Arch. Pharm. Res. 16, 18-24. https://doi.org/10.1007/BF02974122
  12. Kwak, W. J., Han, C. K., Chang, H. W., Kim, H. P., Kang, S. S. and Son, K. H. (2003a). Loniceroside C, an anti-inflammatory saponin from Lonicera japonica. Chem. Pharm. Bull. 51, 333-335. https://doi.org/10.1248/cpb.51.333
  13. Kwak, W. J., Moon, T. C., Lin, C. X., Rhyn, H. G., Jung, H., Lee, E., Kwon, D. Y., Son, K. H., Kim, H. P., Kang, S. S., Murakami, M., Kudo, I. and Chang, H. W. (2003b). Papyriflavonol A from Broussonetia papyrifera inhibits the passive cutaneous anaphylaxic reaction and has a secretory phospholipase $A_2$ inhibitory activity. Biol. Pharm. Bull. 26, 299-302. https://doi.org/10.1248/bpb.26.299
  14. Lee, S. J., Shin, E. J., Son, K. H., Chang, H. W., Kang, S. S. and Kim, H. P. (1995). Anti-inflammatory activity of the major constituents of Lonicera japonica. Arch. Pharm. Res. 18, 133-135. https://doi.org/10.1007/BF02979147
  15. Lee, S. J., Son, K. H., Chang, H. W., Kang, S. S. and Kim, H. P. (1998). Anti-inflammatory activity of Lonicera japonica. Phytother. Res. 12, 445-447. https://doi.org/10.1002/(SICI)1099-1573(199809)12:6<445::AID-PTR317>3.0.CO;2-5
  16. Lin, L. W., Chen, H. Y., Wu, C. R., Liao, P. M., Lin, Y. T., Hsieh, M. T. and Ching, H. (2008). Comparison with various parts of Broussonetia papyrifera as to the antinociceptive and anti-inflammatory activities in rodents. Biosci. Biotechnol. Biochem. 72, 2377-2384. https://doi.org/10.1271/bbb.80276
  17. Mossman, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Methods 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  18. Qian, Z. M., Li, H. J., Li, P., Chen, J. and Tang, D. (2007). Simultaneous quantification of seven bioactive components in Caulis Lonicera japonicae by high performance liquid chromatography. Biomed. Chromatogr. 21, 649-654. https://doi.org/10.1002/bmc.803
  19. Shougakukan. (1985). The dictionary of Chinese drugs. vol. 3. pp. 2027-2029. Shanghai Science and Technologic Publishers and Shougakukan, Tokyo.
  20. Son, K. H., Kwon, S. J., Chang, H. W., Kim, H. P. and Kang, S. S. (2001). Papyriflavonol A, a new prenylated flavonoid from Broussonetia papyrifera. Fitoterapia 72, 456-458. https://doi.org/10.1016/S0367-326X(00)00329-4
  21. Tries, S., Neupert, W. and Laufer, S. (2002). The mechanism of action of the new anti-inflammatory compound ML3000: inhibition of 5-LOX and COX-1/2. Inflamm. Res. 51, 135-143. https://doi.org/10.1007/PL00000285
  22. Vinegar, R., Schreiber, W. and Hugo, R. (1969). Biphasic development of carrageenin edema in rats. J. Pharmacol. Exp. Ther. 166, 96-103.
  23. Wang, J. P., Tsao, L. T., Raung, S. L. and Lin, C. N. (1997). Investigation of the inhibitory effect of broussochalcone A on respiratory burst in neutrophils. Eur. J. Pharmacol. 320, 201-208. https://doi.org/10.1016/S0014-2999(96)00888-6
  24. Winter, C. A., Risley, E. A. and Nuss, G. W. (1962). Carrageenan-induced edema in the hindpaw on the rat as an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med. 111, 544-547. https://doi.org/10.3181/00379727-111-27849

Cited by

  1. Stability and Accuracy Assessment of Identification of Traditional Chinese Materia Medica Using DNA Barcoding: A Case Study on Flos Lonicerae Japonicae vol.2013, 2013, https://doi.org/10.1155/2013/549037
  2. Characterization and anti-allergic effect of a polysaccharide from the flower buds of Lonicera japonica vol.90, pp.4, 2012, https://doi.org/10.1016/j.carbpol.2012.07.044
  3. Inhibition of Experimental Systemic Inflammation (Septic Inflammation) and Chronic Bronchitis by New Phytoformula BL Containing Broussonetia papyrifera and Lonicera japonica vol.21, pp.1, 2013, https://doi.org/10.4062/biomolther.2012.081
  4. Inhibition of Experimental Lung Inflammation and Bronchitis by Phytoformula Containing Broussonetia papyrifera and Lonicera japonica vol.19, pp.3, 2011, https://doi.org/10.4062/biomolther.2011.19.3.324
  5. Dipterocarpus tuberculatus ethanol extract strongly suppresses in vitro macrophage-mediated inflammatory responses and in vivo acute gastritis vol.146, pp.3, 2013, https://doi.org/10.1016/j.jep.2013.01.033
  6. Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis vol.11, pp.11, 2010, https://doi.org/10.3390/ijms11114750
  7. Anti-inflammatory activity of ethanol extract derived from Phaseolus angularis beans vol.137, pp.3, 2011, https://doi.org/10.1016/j.jep.2011.07.048
  8. The Genus Broussonetia: A Review of its Phytochemistry and Pharmacology vol.26, pp.1, 2012, https://doi.org/10.1002/ptr.3575
  9. A Sensitive and Rapid UPLC-MS/MS Method for Determination of Monosaccharides and Anti-Allergic Effect of the Polysaccharides Extracted from Saposhnikoviae Radix vol.23, pp.8, 2018, https://doi.org/10.3390/molecules23081924
  10. Biomass and Flavonoid Production of Gynura procumbens (L.). Merr Adventitious Root Culture in Baloon-type Bubble-bioreactor Influenced by Elicitation vol.17, pp.2, 2018, https://doi.org/10.3923/ajps.2018.107.119
  11. Therapeutic Effect of Broussonetia papyrifera and Lonicera japonica in Ovalbumin-induced Murine Asthma Model vol.8, pp.11, 2013, https://doi.org/10.1177/1934578x1300801127
  12. Effect of Carbon Source on Biomass and Flavonoid Content of Gynuraprocumbens (Lour.) Merr Adventitious Root in Liquid Culture vol.16, pp.1, 2019, https://doi.org/10.13005/bbra/2729