Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan Parasite Entamoeba histolytica

  • Sim, Seo-Bo (Department of Environmental and Tropical Medicine, School of Medicine, Konkuk University) ;
  • Yu, Jae-Ran (Department of Environmental and Tropical Medicine, School of Medicine, Konkuk University) ;
  • Lee, Young-Ah (Department of Environmental Medical Biology,Institute of Tropical Medicine, and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
  • Shin, Myeong-Heon (Department of Environmental Medical Biology,Institute of Tropical Medicine, and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine)
  • Received : 2010.09.24
  • Accepted : 2010.10.13
  • Published : 2010.12.31
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Abstract

Tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. However, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by Entamoeba histolytica, In this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. Entamoeba-induced receptor shedding of CD16 and PS externalization in neutrophils were inhibited by pre-incubation of neutrophils with the broad-spectrum tyrosine kinase inhibitor genistein or the Src family kinase inhibitor PP2. Entamoeba-induced ROS production was also inhibited by genistein or PP2, Moreover, genistein and PP2 blocked the phosphorylation of ERK and p38 MAPK in neutrophils induced by E. histolytica. These results suggest that Src tyrosine kinases may participate in the signaling event for ROS-dependent activation of MAPKs during neutrophil apoptosis induced by E. histolytica.

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References

  1. Guo X, Houpt E, Petri WAJr. Crosstalk at the initial encounter: interplay between host defense and ameba survival strategies. Curr Opin Immunol 2007; 19: 376-384. https://doi.org/10.1016/j.coi.2007.07.005
  2. Dey I, Chadee K. Prostaglandin E2 produced by Entamoeba hiswlytica binds to EP4 receptors and stimulates interleukin-8 production in human colonic cells. Infect Immun 2008; 76: 5158- 5163. https://doi.org/10.1128/IAI.00645-08
  3. Zhelev DV, Alteraifi A. Signaling in the motility responses of the human neutrophil. Ann Biomed Eng 2002; 30: 356-370. https://doi.org/10.1114/1.1477446
  4. Jarillo-Luna RA, Campos-Rodriguez R, Tsutsumi V. Entamoeba hiswlytica: immunohistochemical study of hepatic amoebiasis in mouse. Neutrophils and nitric oxide as possible factors of resistance. Exp Parasitol 2002; 101: 40-56. https://doi.org/10.1016/S0014-4894(02)00021-8
  5. Rivero-Nava L, Aguirre-Garcia J, Shibayama-Salas M, Hernandez-Pando R Tsutsumi V, Calderon J. Entamoeba hiswlytica: acute granulomatous intestinal lesions in normal and neutrophil-depleted mice. Exp Parasitol 2002; 101: 183-192. https://doi.org/10.1016/S0014-4894(02)00106-6
  6. Dickson-Gonzalez SM, de Uribe ML, Rodriguez-Morales AT. Polymorphonuclear neutrophil infiltration intensity as consequence of Entamoeba hiswlytica density in amebic colitis. Surg Infect (Larchmt) 2009; 10: 91-97. https://doi.org/10.1089/sur.2008.011
  7. Ventura-Juarez J, Barba-Gallardo LF, Munoz-Fernandez L, Martinez- Medina L, Marquez-Diaz F, Sosa-Diaz SJ, Gerardo-Rodriguez M, Gonzalez-Romo R Campos-Rodriguez R. Immunohistochemical characterization of human fulminant amoebic colitis. Parasite Immunol 2007; 29: 201-209. https://doi.org/10.1111/j.1365-3024.2007.00934.x
  8. Singer II, Kawka DW, Schloemann S, Tessner T, Riehl T, Stenson WF. Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatOIY bowel disease. Gastroenterology 1998; 115: 297-306. https://doi.org/10.1016/S0016-5085(98)70196-9
  9. Sim S, Kim KA, Yong TS, Park SJ, Im KI, Shin MH. Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba hiswlytica. Korean J Parasitol 2004; 42: 205-208. https://doi.org/10.3347/kjp.2004.42.4.205
  10. Sim S, Yong TS, Park SJ, Im KI, Kong Y, Ryu JS, Min DY, Shin MH. NADPH oxidase-derived reactive oxygen species-mediated activation of ERK1/2 is required for apoptosis of human neutrophils induced by Entamoeba hiswlytica. J Immunol 2005; 174: 4279-4288. https://doi.org/10.4049/jimmunol.174.7.4279
  11. Okutani D, Lodyga M, Han B, Liu M. Src protein tyrosine kinase family and acute inflammatory responses. Am J Physiol Lung Cell Mol Physiol 2006; 291: L129-141. https://doi.org/10.1152/ajplung.00261.2005
  12. Jin T, Xu X, Fang J, Isik N, Yan J, Brzostowski JA, Hereld D. How human leukocytes track down and destroy pathogens: lessons learned from the model organism Dictyostelium discoideum. Immunol Res 2009; 43: 118-127. https://doi.org/10.1007/s12026-008-8056-7
  13. Lacy P, Eitzen G. Control of granule exocytosis in neutrophils. Front Biosci 2008; 13: 5559-5570.
  14. Yousefi S, Green DR Blaser K, Simon HU. Protein-tyrosine phosphorylation regulates apoptosis in human eosinophils and neutrophils. Proc Natl Acad Sci USA 1994; 91: 10868-10872. https://doi.org/10.1073/pnas.91.23.10868
  15. Sim S, Park SJ, YongTS, Im KI, Shin MH. Involvement of beta 2- integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica. Microbes Infect 2007; 9: 1368-1375. https://doi.org/10.1016/j.micinf.2007.06.013
  16. Lacy P. Mechanisms of degranulation in neutrophils. Allergy Asthma Clin Immunol 2006; 2: 98-108. https://doi.org/10.1186/1710-1492-2-3-98
  17. Lowell CA. Src-family kinases: rheostats of immune cell signaling. Mol Immunol 2004; 41: 631-643. https://doi.org/10.1016/j.molimm.2004.04.010
  18. Mocsai A, Jakus Z, Vantus T, Berton G, Lowell CA, Ligeti E. Kinase pathways in chemoattractant-induced degranulation of neutrophils: the role of p38 mitogen-activated protein kinase activated by Src family kinases. J Immunol 2000; 164: 4321-4331. https://doi.org/10.4049/jimmunol.164.8.4321
  19. Coxon A, Rieu P, Barkalow FJ, Askari S, Sharpe AH, von Andrian UH, Amaout MA, Mayadas TN. A novel role for the ${\beta}_2$, integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation. Immunity 1996; 5: 653-666. https://doi.org/10.1016/S1074-7613(00)80278-2
  20. Berton G, Fumagalli L, Laudanna C, Sorio C. ${\beta}_2$, integrin-dependent protein tyrosine phosphorylation and activation of the FGR protein tyrosine kinase in human neutrophils. J Cell Biol 1994; 126: 1111-1121 https://doi.org/10.1083/jcb.126.4.1111
  21. Lowell CA, Fumagalli L, Berton G. Deficiency of Src family kinases p59/61hck and p58c-fgr results in defective adhesion-dependent neutrophil functions. J Cell Biol 1996; 133: 895-910. https://doi.org/10.1083/jcb.133.4.895
  22. Mocsai A, Zhou M, Meng F, Tybulewicz VL, Lowell CA. Syk is required for integrin signaling in neutrophils. Immunity 2002; 16: 547-558. https://doi.org/10.1016/S1074-7613(02)00303-5
  23. Mocsai A, ligeti E, Lowell CA, Berton G. Adhesion-dependent degranulation of neutrophils requires the Src family kinases Fgr and Hck. J Immunol 1999; 162: 1120-1126.
  24. Pereira S, Zhou M, Mocsai A, Lowell C. Resting murine neutrophils express functional alpha 4 integrins that signal through Src family kinases. J Immunol 2001; 166: 4115-4123. https://doi.org/10.4049/jimmunol.166.6.4115
  25. Moon KD, Post CB, Durden DL, Zhou Q, De P, Harrison ML, Geahlen RL. Molecular basis for a direct interaction between the Syk protein-tyrosine kinase and phosphoinositide 3-kinase. J Biol Chem 2005; 280: 1543-1551. https://doi.org/10.1074/jbc.M407805200
  26. Kim LC, Song L, Haura EB. Src kinases as therapeutic targets for cancer. Nat Rev Clin Oncol 2009; 6: 587-595. https://doi.org/10.1038/nrclinonc.2009.129
  27. Laudanna C, Rossi F, Berton G. Effect of inhibitors of distinct signalling pathways on neutrophil $Q_2$-generation in response to tumor necrosis factor-a, and antibodies against CD18 and CD11a: evidence for a common and unique pattem of sensitivity to wortmannin and protein tyrosine kinase inhibitors. Biochem Biophys Res Commun 1993; 190: 935-940. https://doi.org/10.1006/bbrc.1993.1139
  28. D'Aura Swanson C, Paniagua RT, Lindstrom TM, Robinson WH. Tyrosine kinases as targets for the treatment of rheumatoid arthritis. Nat Rev Rheumatol 2009; 5: 317-324. https://doi.org/10.1038/nrrheum.2009.82
  29. Mocsai A, Ruland J, Tybulewicz VL. The SYK tyrosine kinase: a aucial player in diverse biological functions. Nat Rev Immunol 2010; 10: 387-402. https://doi.org/10.1038/nri2765

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