Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Cryopreservation of Semen in Dead Yellow Croaker, Larimichthys polyactis

  • Lim, Han-Kyu (Aquaculture Industry Division, West Sea Fisheries Institute, NFRDI) ;
  • Min, Byung-Hwa (Aquaculture Industry Division, East Sea Fisheries Institute, NFRDI) ;
  • Jeong, Min-Hwan (Aquaculture Management Division, NFRDI) ;
  • Choi, Byul-Nim (Aquaculture Management Division, NFRDI) ;
  • Le, Minh Hoang (Department of Marie Biomaterials and Aquaculture, Pukyong National University) ;
  • Chang, Young-Jin (Department of Marie Biomaterials and Aquaculture, Pukyong National University)
  • Received : 2010.08.19
  • Accepted : 2010.12.10
  • Published : 2010.12.31
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Abstract

This study demonstrated that cryopreserved semen from dead fish can be used for seedling production. Yellow croakers, Larimichthys polyactis, were killed and stored at temperatures of $20^{\circ}C$ or $0^{\circ}C$ for 6 hours. At 2 hour intervals, semen from these fish was collected using abdominal pressure and evaluated for spermatozoa motility and semen cryopreservation. Semen collected after 6 hours from dead fish stored at $0^{\circ}C$ could be cryopreserved and attained fertilization and hatching rates of $15.0{\pm}1.2%$ and $14.8{\pm}1.6%$, respectively. This study suggests that germ cells such as the semen of dead fish can be cryopreserved and utilized in the restoration of a species.

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References

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Cited by

  1. Ultrastructural studies of spermatogenesis and the functions of Leydig cells and Sertoli cells associated with spermatogenesis in Larimichthys polyactis (Teleostei, Perciformes, Sciaenidae) vol.17, pp.4, 2010, https://doi.org/10.1080/19768354.2013.829783